LOCUS       sequence-31268-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-ACP
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(409..427)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      423..470
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(645..650,651..667,668..673))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(700..718)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat accatgcatc atcatcatca tcacagcagc
       61 ggcagagaaa acttgtattt ccagggcagc agtgacgatc ggccactgct ggaacgtgtt
      121 aaggacgtcg tagcagatca attaggcgtg gaccgcgctc gtatcaatcc tgagtcaaac
      181 ttcattaaag atctggatgc ggactccctg gattcggttg aattagtcat ggcctttgaa
      241 gagaaattcg gtgtgagcat cccggacgaa gaggcatcta agattgcgac agtacaggat
      301 gctctgagtt acatcgaaaa agccaaatca tgatgacgaa gcttgcggcc gcactcgagg
      361 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
      421 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
      481 gaactatatc cggatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc
      541 tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca
      601 cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat
      661 gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
      721 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
      781 atgtgcgcgg aaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      841 atgagac
//
