LOCUS       sequence-31269-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-CDPK1 (3HZT)
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgcatc atcatcatca
       61 tcacagcagc ggcagagaaa acttgtattt ccagggcctg tccgatcgct accaacgtgt
      121 taaaaaactg gggtccggag cttatggcga agtcctgctg tgtaaagaca aactgacggg
      181 tgctgagcgc gcgatcaaaa ttatcaaaaa aagctctgta actacaacca gtaacagtgg
      241 tgcgctgctg gatgaagtgg cagttctgaa acagctggat catccgaata ttatgaaact
      301 gtacgaattt ttcgaggaca aacgtaacta ttacctggtc atggaagtat atcgcggtgg
      361 ggagctgttt gatgaaatca ttctgcgtca gaaattcagt gaagtggacg ctgcggttat
      421 catgaaacaa gtcctgagcg gcactaccta cctgcacaaa cataatattg tgcaccgcga
      481 tctgaaaccg gagaacctgc tgctggaaag caaatctcgt gacgccctga tcaaaattgt
      541 agattttggc ctgagtgcac atttcgaggt tggtgggaaa atgaaagaac gcctgggcac
      601 tgcttattat atcgcgccag aagtgctgcg taaaaaatac gatgagaaat gcgacgtctg
      661 gtcatgtggt gttattctgt atatcctgct gtgcggatac cctccgtttg gcggtcagac
      721 agatcaagaa attctgaaac gcgtagagaa agggaaattt tcctttgacc cgccagattg
      781 gacgcaggtg tcagatgaag ccaaacagct ggtcaaactg atgctgacct atgaaccttc
      841 gaaacgtatc tctgcagagg aagcgctgaa tcacccgtgg attgttaaat tctgtagcca
      901 gaaacatact gacgtgggca aacacgctct gacaggtgcc ctgggaaaca tg
//
