LOCUS       sequence-31272-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-CDPK4 (3IGO)
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctgaatattt tgtttacttt agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca cctttgcaga aaggtacaat attgtatgta
      121 tgcttggcaa aggttctttt ggagaagttt tgaaatgtaa agacagaatc actcaacaag
      181 aatatgctgt caaggttatt aacaaagcta gtgcaaagaa caaggatact agtacaattt
      241 tgagagaagt tgagctttta aagaagcttg atcatccaaa tattatgaag ctattcgaga
      301 ttttggaaga ttcatctagc ttctacattg taggagagct ttacacagga ggagaactct
      361 ttgatgaaat tattaagaga aaaagattta gtgagcatga tgctgctaga attattaaac
      421 aagtattttc aggaattact tacatgcata aacataacat tgtacataga gatttaaagc
      481 cagaaaacat acttttagag tctaaagaaa aagactgtga tattaaaatt atagattttg
      541 gattatcaac atgcttccag caaaatacca agatgaagga tagaatagga acagcctact
      601 atatagctcc agaagttttg agaggtactt atgatgagaa atgtgatgta tggtcagctg
      661 gagtaattct atatattctc ttatctggaa cacctccatt ctatggaaaa aatgaatatg
      721 atattttgaa gagagtcgag acaggaaagt atgcttttga ccttccacaa tggagaacta
      781 tttctgatga tgccaaggat ttaataagaa agatgttaac tttccatcct tctttgagaa
      841 ttactgcaac acaatgttta gaacatccat ggattcaaaa atattcaagt gagactccaa
//
