LOCUS       sequence-312727-        2046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC5A9_STOP
FEATURES             Location/Qualifiers
     source          1..2046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgagcaagg agctggccgc catgggacca ggagcatccg gcgacggcgt gcggaccgag
       61 acagcccccc acatcgccct ggactccaga gtgggactgc acgcctacga tatctctgtg
      121 gtggtcatct acttcgtgtt tgtgatcgcc gtgggcatct ggagctccat cagggcaagc
      181 aggggaacca tcggaggata cttcctggcc ggcagatcca tgtcttggtg gcccatcggc
      241 gcctctctga tgtctagcaa cgtgggaagc ggactgttta tcggactggc aggcacagga
      301 gcagcaggag gactggcagt gggaggattc gagtggaatg caacctggct gctgctggcc
      361 ctgggatggg tgtttgtgcc cgtgtacatc gcagcaggag tggtgacaat gcctcagtat
      421 ctgaagaagc ggttcggcgg ccagagaatc caggtgtaca tgagcgtgct gtccctgatc
      481 ctgtatatct ttacaaagat ctctaccgat atcttcagcg gcgccctgtt tatccagatg
      541 gccctgggct ggaacctgta tctgagcacc ggcatcctgc tggtggtgac agccgtgtac
      601 accatcgccg gcggcctgat ggccgtgatc tatacagacg ccctgcagac cgtgatcatg
      661 gtgggaggcg ccctggtgct gatgttcctg ggctttcagg atgtgggatg gtacccagga
      721 ctggagcagc ggtatagaca ggccatcccc aacgtgacag tgcctaatac cacatgccac
      781 ctgccaaggc cagacgcatt ccacatcctg agggaccccg tgagcggcga tatcccttgg
      841 ccaggcctga tctttggcct gacagtgctg gccacctggt gctggtgtac cgatcaggtc
      901 atcgtgcagc ggagcctgtc cgccaagtct ctgagccacg caaagggagg atccgtgctg
      961 ggaggctacc tgaagatcct gcccatgttc tttatcgtga tgcctggcat gatctctaga
     1021 gccctgttcc cagacgaagt gggatgcgtg gacccagacg tgtgccagag gatctgtgga
     1081 gcaagggtgg gatgtagcaa catcgcatat cctaagctgg tcatggcact gatgccagtg
     1141 ggactgaggg gactgatgat cgccgtgatc atggccgccc tgatgtcctc tctgacaagc
     1201 atcttcaata gctcctctac cctgtttaca atcgacgtgt ggcagaggtt ccggagaaag
     1261 tccaccgagc aggagctgat ggtggtggga cgcgtgttcg tggtgtttct ggtggtcatc
     1321 tctatcctgt ggatccctat catccagagc tccaacagcg gccagctgtt tgattacatc
     1381 caggccgtga catcctatct ggccccccct atcaccgccc tgttcctgct ggccatcttt
     1441 tgcaagaggg tgaccgagcc aggagcattc tggggactgg tgtttggact gggagtggga
     1501 ctgctgagaa tgatcctgga gttctcctac cccgcccctg catgtggaga ggtggacagg
     1561 cgccccgccg tgctgaagga tttccactac ctgtattttg ccatcctgct gtgcggcctg
     1621 acagccatcg tgatcgtgat cgtgtctctg tgcaccacac caatccccga ggagcagctg
     1681 acccggctga catggtggac cagaaactgc cctctgtccg agctggagaa ggaggcccac
     1741 gagtccaccc cagagatctc tgagaggcca gcaggagagt gtccagcagg aggaggagca
     1801 gccgagaatt ctagcctggg acaggagcag cctgaggcac catcccgctc ttggggcaag
     1861 ctgctgtgga gctggttctg cggactgtcc ggaacacctg agcaggccct gtctccagca
     1921 gagaaggccg ccctggagca gaagctgacc agcatcgagg aggagccact gtggaggcac
     1981 gtgtgcaaca tcaatgccgt gctgctgctg gccatcaata tcttcctgtg gggctacttt
     2041 gcctaa
//
