LOCUS       sequence-31275-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-CPUF2
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..78
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..105
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             384..401
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(454..472)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      468..515
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      552..910
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(639..658)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     849..870
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gggtaattcc tctgaaatat tttgtttact ttaagaagga gatataccat gggcagcagc
       61 catcatcatc atcatcacag cagcggcctg gttccgcgtg gtagttgcat acaaaaggtt
      121 attgaagata agttaagttc agctttaaag cccacatttt tagaattagt agataaatct
      181 tgtggatgtg gaacatcctt tgatgctgtc atagtttcta ataatttcga ggataaaaaa
      241 ttattagata gacatagact tgttaacact atattaaaag aagaactaca aaatattcac
      301 gccttttcta tgaaatgtca caccccactg gaatatgata aattaaaaag caaatgatga
      361 cgaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca
      421 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
      481 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
      541 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
      601 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      661 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      721 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      781 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      841 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      901 ttttgattta taagggattt tgccga
//
