LOCUS       sequence-31276-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-HSP40
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(712..730)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      726..773
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attccctctg aatattttgt ttactttaga aggagatata ccatgcatca tcatcatcat
       61 cacagcagcg gcagagaaaa cttgtatttc cagggcgccc caagatcgca cgaagtccca
      121 ttattggtaa ctttagaaga gctatactta gggaaacgta agaaaattaa ggttacaaga
      181 aaaaggttta tagaacataa agtgaggaat gaagaaaata tagtcgaggt agaaataaaa
      241 cctggctgga aagacgggac caagttaaca tattctggag aaggtgatca ggaatcacct
      301 ggaacgtcgc ctggagattt agtacttata attcaaacaa agactcaccc cagattcaca
      361 agagatgact gccatcttat tatgaaggta actatcccct tagttagagc gctaacaggt
      421 tttacatgtc ctgtgacaac tcttgacaat cgtaacttac agatcccaat caaggaaatt
      481 gtaaatccaa agacaagaaa aattgttcca aatgaaggga tgccaataaa gaatcagcca
      541 ggacaaaaag gagacctaat acttgaattt gatatttgtt ttccaaagtc tctcacacca
      601 gagcagaaga agcttattaa agaagcatta gactgatgac gaagcttgcg gccgcactcg
      661 aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
      721 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      781 gaggaactat atccggatat cccgcaagag gcccggcagt accggcataa ccaagcctat
      841 gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcat
//
