LOCUS       sequence-31277-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PPI63
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..93
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             642..659
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(712..730)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      726..773
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(897..916)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctgaatattt tgtttacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat
       61 catcacagca gcggcctggt tccgcgtggt agtccacagt gccatttcga cattgaaatc
      121 aaccgtgagc cagttgggcg gataatgttt cagctgttca gcgacatttg cccgaaaact
      181 tgcaaaaact tcctgtgtct gtgtagtggc gaaaaaggtc tgggaaaaac caccggtaaa
      241 aaactgtgct acaaaggatc aacattccat cgcgtggtga aaaactttat gatccagggc
      301 ggcgattttt ccgaaggtaa cggcaagggt ggggaatcca tttatggcgg ctattttaaa
      361 gatgaaaatt ttatcctgaa acatgatcgc gccttcctgc taagcatggc gaatcgtggt
      421 aaacacacga atgggagcca gttctttatt acgaccaaac cggccccaca cctggatgga
      481 gtgcatgtgg tgtttggttt ggtaatctcc ggttttgaag tcatcgaaca gattgaaaac
      541 ctgaaaacgg atgccgcaag tcgcccgtat gccgatgtgc gcgttattga ttgtggcgtt
      601 ttggcgacca aatgatgacg aagcttgcgg ccgcactcga gcaccaccac caccaccact
      661 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
      721 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      781 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
      841 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
      901 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
      961 tc
//
