LOCUS       sequence-31278-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PPI65
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             654..671
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(724..742)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      738..785
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggttcc gcgtggtagt ggctacgtgc ggctgcacac caacaagggc
      121 gacctcaacc tggagctgca ctgcgacctg acaccaaaaa cctgcgaaaa cttcatcagg
      181 ctttgcaaga agcattatta cgatggcacc atcttccaca gatccatccg gaactttgtg
      241 atccaagggg gcgaccccac aggcacaggc acgggtgggg agtcatactg ggggaagccc
      301 ttcaaagacg agttccggcc caacctctcg cacacgggcc gcggcatcct cagcatggcc
      361 aactccgggc ccaacagcaa caggtctcaa ttcttcatca cgtttcgctc ctgtgcctac
      421 ctggacaaga agcataccat ctttggacgg gttgttgggg gctttgacgt actgacagcc
      481 atggagaatg tggagagtga ccccaaaact gaccgcccta aggaggagat ccgcattgat
      541 gccactacag tgttcgtgga cccctatgag gaggccgatg cccagattgc gcaggagcgg
      601 aagacacagc tcaaggtagc cccgtgatga cgaagcttgc ggccgcactc gagcaccacc
      661 accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
      721 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
      781 ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc
      841 attaagcgcg gcggttgtgg tggttac
//
