LOCUS       sequence-31279-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FKBP02
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..89
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             419..436
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(489..507)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      503..550
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(674..693)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tattttgttt aactttaaga aggagatata ccatgggcag cagccatcat catcatcatc
       61 acagcagcgg cctggttccg cgtggtagtc ccatcaaatc gcgcaaaggg gatgtcctgc
      121 acatgcacta cacggggaag ctggaagatg ggacagagtt tgacagcagc ctgccccaga
      181 accagccctt tgtcttctcc cttggcacag gccaggtcat caagggctgg gaccaggggc
      241 tgctggggat gtgtgagggg gaaaagcgca agctggtgat cccatccgag ctagggtatg
      301 gagagcgggg agctccccca aagattccag gcggtgcaac cctggtgttc gaggtggagc
      361 tgctcaaaat agagcgacga actgagctgt gatgacgaag cttgcggccg cactcgagca
      421 ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
      481 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
      541 gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc
      601 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      661 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      721 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
      781 ctcga
//
