LOCUS       sequence-31283-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-RAB1A
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(712..730)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      726..773
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 acattccctc tgaatatttt gtttacttta agaaggagat ataccatgca tcatcatcat
       61 catcacagca gcggcagaga aaacttgtat ttccagggca tgaatcctga gtacgactac
      121 ttattcaaac tacttttaat aggagatagt ggtgtaggta aatcatgctt actcttacga
      181 tttgctgatg acacctatac tgacagttat atttcaacaa ttggggttga tttcaagata
      241 agaacaataa gtttggagaa taagacagta aagttacaga tttgggatac tgcaggacaa
      301 gaaaggttta gaacaattac ttctagttat tatagagggg cacatggaat cattattgtc
      361 tatgatgtta cagatagaga ttcttttgat aatgttaaac aatggatcca agagattgat
      421 agatacgcaa tggagaatgt taataaattg ttagtaggta ataagtgcga tcttgtgagc
      481 aagagagtgg taacatcaga cgagggaaga gaacttgcag attctcatgg aataaaattt
      541 atagaaactt ctgctaaaaa cgcttataat gttgagcaag cattccatac aatggctggt
      601 gagatcaaga agagagttca agtgaatagt cagtgatgac gaagcttgcg gccgcactcg
      661 aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
      721 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      781 gaggaactat atccggatat cccgcaagag gcccggcagt accggcataa ccaagcctat
      841 gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat
      901 acacggtgcc tgact
//
