LOCUS       sequence-31284-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-SIR2
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..93
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cctctgaata ttttgtttac tttaagaagg agatatacca tgcatcatca tcatcatcac
       61 agcagcggca gagaaaactt gtatttccag ggcaaaaaag acacacaaag tattactttg
      121 gaggagctag ccaaaataat aaaaaaatgc aaacatgtag ttgccttaac agggtcaggt
      181 acatctgcag aaagtaacat cccaagtttt cgagggtcat cgaattcgat atggagtaag
      241 tatgacccaa gaatatatgg aactatatgg ggtttttgga agtatcctga aaagatatgg
      301 gaagtaataa gagatatatc atccgattat gaaatagaaa taaacaatgg tcatgtagct
      361 ttatcaactt tagaaagttt gggttattta aaatcagtag taacacaaaa tgtagatgga
      421 ttacatgaag ctagtgggaa tacaaaagtt atatcattac atgggaatgt atttgaagca
      481 gtatgttgta catgtaataa aattgtaaaa ttaaataaaa tcatgttaca gaaaacatct
      541 cattttatgc atcaattacc acctgaatgt ccttgtggag gaatattcaa accaaacatt
      601 atattatttg gagaggttgt ttcatcagat cttttaaaag aagcagaaga agaaatagca
      661 aaatgtgatt tacttttagt aattggcaca tcgtctactg tatcaacagc tactaatttg
      721 tgtcatttcg cttgtaagaa aaaaaaaaaa attgtggaaa ttaatatatc caaaacatat
      781 ataacaaaca aaatgtctga ttatcatgta tgtgcnaaat ttagtgaatt aactaaagtg
      841 gcaaatatat taaaagggtc aagtg
//
