LOCUS       sequence-31287-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PRDM10
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             585..602
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(655..673)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      669..716
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(840..859)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aaattccctc tgaatatttt gtttacttta agaaggagat ataccatgca tcatcatcat
       61 catcacagca gcggcagaga aaacttgtat ttccagggca agcacggccc cttgcacccg
      121 atccccaacc ggccggtgct cacccgggcc agggcgagcc tccccctggt gctctacata
      181 gacaggtttc tgggcggggt gttctccaag cggcgcatcc ccaagcgcac ccagtttggc
      241 cccgtggagg ggcctctcgt caggggctcg gagctgaaag actgttacat tcacctcaag
      301 gtttctcttg ataaagggga caggaaagaa agggatttac atgaagacct atggtttgag
      361 ttgtctgatg agacgctttg taactggatg atgtttgtac ggccagccca gaatcacctg
      421 gagcagaacc tggtggctta ccagtatggc caccatgtgt attatacaac cataaaaaat
      481 gtggagccca agcaggaact gaaggtgtgg tatgccgcat cctatgctga gttcgtgaac
      541 cagaaaattc atgactgatg acgaagcttg cggccgcact cgagcaccac caccaccacc
      601 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      661 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      721 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
      781 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      841 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
      901 aaatcggggg ctccctttag ggttcga
//
