LOCUS       sequence-31288-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RICS
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             65..85
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             718..735
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(788..806)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tattttgttt actttaagaa ggagatatac catgcatcat catcatcatc acagcagcgg
       61 cagagaaaac ttgtatttcc agggcgaacg tgtgtttggt tgcgacctgg gcgagcatct
      121 gcttaattct ggcttcgaag ttccccaggt cctgcaaagt tgtaccgctt ttatcgagcg
      181 ctacggtatt gtagacggga tctaccgtct gagcggcgtc gcttccaaca tccaacgtct
      241 gcgtcacgag ttcgattcgg aacacgttcc ggatctgact aaagagccgt acgtccagga
      301 tatccacagc gtaggttctc tgtgcaaact gtactttcgc gagctgccga atccgctgct
      361 gacatatcaa ctgtacgaga agttctcaga cgctgtatca gctgcgacag acgaagaacg
      421 tctgattaag atccacgacg ttatccaaca actgccgccg ccgcactatc gtacgctgga
      481 gttcctgatg cgtcatctgt ccctgctggc cgactactgt tcgatcacta acatgcacgc
      541 aaagaacctg gctatcgtat gggcaccgaa ccttctgcgc tcaaagcaga ttgaatctgc
      601 ctgcttctct ggcacagcag cattcatgga ggtgcgtatc caaagtgttg ttgtggagtt
      661 catcctgaat cacgtcgacg ttctgttttg atgacgaagc ttgcggccgc actcgagcac
      721 caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
      781 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttga
//
