LOCUS       sequence-312898-        9730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for intestinal stem cells through ASCL2 
            transcriptional activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Heinz MC, Oost KC, Snippert HJG
  TITLE     Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal 
            Organoids.
  JOURNAL   STAR Protoc. 2020 Oct 8;1(3):100126. doi: 
            10.1016/j.xpro.2020.100126. eCollection 2020 Dec 18.
  PUBMED    33377020
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2020-10-8"; volume: "1(3):100126. doi"; pages: " 
            10.1016/j.xpro.2020.100126. eCollection 2020 Dec 18"
COMMENT     Sequence Label: pLV 4xSTAR-mTFP-NLS-blast
FEATURES             Location/Qualifiers
     source          1..9730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1212..1232
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    1282..1834
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1426..1443)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1653..1672
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      1855..1864
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1861..2259
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3102..3335
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3407..3528
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3444..3463)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3498..3517
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3568..3703
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3723..3742)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3724..3742)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3752..3769)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3752..3768)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3761..3783)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3910..4365
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3997..4016)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4207..4228
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4391..4495
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4496..4564,4565..5356)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4714..4733)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5527..6115
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6016..6035
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6269..6286
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6403..6424
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6439..6444,6445..6462,6463..6469)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6477..6493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6482..6504
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6501..6517
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6501..6517
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7874..7894
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7876..7894
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7922..8148
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8149..8329
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8376..8501
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8994..9227
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9412..9456
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(9718..9730,1..105)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
       61 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tcggtacccc
      121 tagggtgaaa aggcaggtgc aaagcaaatc ctatcccagc ttctagctag gtgaaaaggc
      181 aggtgcaaag caaatcctat cccagcttct agctagggtg aaaaggcagg tgcaaagcaa
      241 atcctatccc agcttctagc taggtgaaaa ggcaggtgca aagcaaatcc tatcccagct
      301 tctagctagc ggcgcgccgg atccggaggg gacaagtgga gggaaagtgg ggccgggcgg
      361 ggggtgcctg ggaagccagg ctgcgctgac gtcactgggc gcgcaattcg ggctggagcg
      421 ctttaaaaaa cgagcgtgca agcagagagg ctgctccaca ccgctcaggc cgcggtttaa
      481 acatcgatat ggccgcctca aaaggagaag aaacgacaat gggtgttatc aagccagaca
      541 tgaagatcaa gcttaaaatg gagggtaatg ttaatggtca cgccttcgtc attgagggag
      601 agggtgaagg aaagccatac gatggaacta atactattaa cttggaagtg aaggagggtg
      661 ccccacttcc attctcttat gatatcctta ctacagcctt cgcatatgga aatcgagcat
      721 tcacaaaata cccagacgac attccaaatt acttcaagca gtcatttcca gagggatatt
      781 cttgggagag aacaatgaca ttcgaggaca agggtatagt taaagtgaaa tctgatatta
      841 gtatggaaga agattcattc atctatgaga tacacctgaa gggcgagaac tttcctccga
      901 atggaccagt gatgcagaaa aagacaacag gatgggacgc aagtactgaa cgtatgtatg
      961 tcagagatgg agttttgaaa ggtgacgtga agcataaact gttgttggaa ggaggtggac
     1021 atcatcgagt ggactttaag acaatctaca gagctaagaa agctgtgaaa ttgccagact
     1081 atcactttgt ggatcaccga attgaaattt tgaaccatga taaagattac aataaagtga
     1141 ctgtgtatga gtcagcagta gctagaaact caactgatgg tatggacgag ctctacgtaa
     1201 gcgcttccag gccaaaaaag aaaagaaagg tcatcgatta gttaattaat ggcccgggaa
     1261 ttccgcccct ctccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     1321 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     1381 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     1441 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     1501 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     1561 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     1621 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     1681 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     1741 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     1801 acgtggtttt cctttgaaaa acacgatgat aatatggata tggccacaac ggtcgccacc
     1861 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
     1921 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
     1981 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
     2041 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
     2101 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
     2161 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
     2221 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaaa tgcatgtatt caatctaagc
     2281 aggctttcac tttctcgcca acttacaagg cctttctgtg taaacaatac ctgaaccttt
     2341 accccgttgc ccggcaacgg ccaggtctgt gccaagtgtt tgctgacgca acccccactg
     2401 gctggggctt ggtcatgggc catcagcgca tgcgtggaac cttttcggct cctctgccga
     2461 tccatactgc ggaactccta gccgcttgtt ttgctcgcag caggtctgga gcaaacatta
     2521 tcgggactga taactctgtt gtcctatccc gcaaatatac atcgtttcca tggctgctag
     2581 gctgtgctgc caactggatc ctgcgcggga cgtcctttgt ttacgtcccg tcggcgctga
     2641 atcctgcgga cgacccttct cggggtcgct tgggactctc tcgtcccctt ctccgtctgc
     2701 cgttccgacc gaccacgggg cgcacctctc tttacgcgga ctccccgtct gtgccttctc
     2761 atctgccgga ccgtgtgcac ttcgcttcac ctctgcacgt cgcatggaga ccaccgtgaa
     2821 cgcccaccaa atattgccca aggtcttaca taagaggact cttggactct cagcaatgtc
     2881 aacgaccgac cttgaggcat acttcaaaga ctgtttgttt aaagactggg aggagttggg
     2941 ggaggagatt aggttaaagg tctttgtact aggaggctgt aggcataaat tggtctgcgc
     3001 accagcacca tggcgcaatc actagagcgg ggtaccttta agaccaatga cttacaaggc
     3061 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     3121 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     3181 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     3241 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     3301 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     3361 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     3421 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     3481 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     3541 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3601 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     3661 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     3721 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     3781 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     3841 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     3901 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     3961 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4021 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4081 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4141 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4201 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     4261 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     4321 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     4381 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     4441 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     4501 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     4561 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     4621 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     4681 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     4741 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     4801 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     4861 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     4921 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     4981 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5041 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5101 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5161 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5221 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     5281 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     5341 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     5401 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     5461 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5521 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     5581 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5641 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     5701 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     5761 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     5821 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     5881 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     5941 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6001 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6061 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6121 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     6181 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     6241 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     6301 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     6361 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     6421 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     6481 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgctgatg
     6541 aatcccctaa tgattttggt aaaaatcatt aagttaaggt ggatacacat cttgtcatat
     6601 gatcaaatgg tttcgcgaaa aatcaataat cagacaacaa gatgtgcgaa ctcgatattt
     6661 tacacgactc tctttaccaa ttctgccccg aattacactt aaaacgactc aacagcttaa
     6721 cgttggcttg ccacgcatta cttgactgta aaactctcac tcttaccgaa cttggccgta
     6781 acctgccaac caaagcgaga acaaaacata acatcaaacg aatcgaccga ttgttaggta
     6841 atcgtcacct ccacaaagag cgactcgctg tataccgttg gcatgctagc tttatctgtt
     6901 cgggcaatac gatgcccatt gtacttgttg actggtctga tattcgtgag caaaaacgac
     6961 ttatggtatt gcgagcttca gtcgcactac acggtcgttc tgttactctt tatgagaaag
     7021 cgttcccgct ttcagagcaa tgttcaaaga aagctcatga ccaatttcta gccgaccttg
     7081 cgagcattct accgagtaac accacaccgc tcattgtcag tgatgctggc tttaaagtgc
     7141 catggtataa atccgttgag aagctgggtt ggtactggtt aagtcgagta agaggaaaag
     7201 tacaatatgc agacctagga gcggaaaact ggaaacctat cagcaactta catgatatgt
     7261 catctagtca ctcaaagact ttaggctata agaggctgac taaaagcaat ccaatctcat
     7321 gccaaattct attgtataaa tctcgctcta aaggccgaaa aaatcagcgc tcgacacgga
     7381 ctcattgtca ccacccgtca cctaaaatct actcagcgtc ggcaaaggag ccatgggttc
     7441 tagcaactaa cttacctgtt gaaattcgaa cacccaaaca acttgttaat atctattcga
     7501 agcgaatgca gattgaagaa accttccgag acttgaaaag tcctgcctac ggactaggcc
     7561 tacgccatag ccgaacgagc agctcagagc gttttgatat catgctgcta atcgccctga
     7621 tgcttcaact aacatgttgg cttgcgggcg ttcatgctca gaaacaaggt tgggacaagc
     7681 acttccaggc taacacagtc agaaatcgaa acgtactctc aacagttcgc ttaggcatgg
     7741 aagttttgcg gcattctggc tacacaataa caagggaaga cttactcgtg gctgcaaccc
     7801 tactagctca aaatttattc acacatggtt acgctttggg gaaattatga ggggatctct
     7861 cagcgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     7921 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     7981 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     8041 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     8101 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     8161 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8221 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8281 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     8341 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     8401 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     8461 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     8521 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     8581 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     8641 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     8701 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     8761 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     8821 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     8881 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     8941 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     9001 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     9061 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     9121 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     9181 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     9241 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     9301 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     9361 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     9421 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     9481 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     9541 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     9601 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     9661 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggttt
     9721 taaaagaaaa
//
