LOCUS       sequence-31290-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TDRD2
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             399..416
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(469..487)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      483..530
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(654..673)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aattttgttt aactttaagg aaggagatat accatgcatc atcatcatca tcacagcagc
       61 ggcagagaaa acttgtattt ccagggcggc tcccgcagcc tgcaattgga taagcttgtc
      121 aatgagatga cccagcacta tgagaatagt gtgcctgaag acttgactgt gcatgtagga
      181 gacattgtag cagcaccttt acctacaaat ggttcctggt atcgagcccg ggtcctcggc
      241 accttggaga atgggaactt ggacctctat tttgttgact ttggagataa tggagattgc
      301 ccactgaagg acctcagggc tctcaggagt gacttcctaa gccttccatt tcaagcaata
      361 gaatgtagtt gatgacgaag cttgcggccg cactcgagca ccaccaccac caccactgag
      421 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
      481 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
      541 gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
      601 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
      661 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
      721 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
      781 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
      841 gttggagtcc acgttcttta atagtggact cttgttccaa act
//
