LOCUS       sequence-312905-        9721 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for intestinal stem cells through ASCL2 
            transcriptional activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Heinz MC, Oost KC, Snippert HJG
  TITLE     Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal 
            Organoids.
  JOURNAL   STAR Protoc. 2020 Oct 8;1(3):100126. doi: 
            10.1016/j.xpro.2020.100126. eCollection 2020 Dec 18.
  PUBMED    33377020
REFERENCE   2  (bases 1 to 9721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2020-10-8"; volume: "1(3):100126. doi"; pages: " 
            10.1016/j.xpro.2020.100126. eCollection 2020 Dec 18"
COMMENT     Sequence Label: pLV 4xSTAR-mNeonGreen-NLS-blast
FEATURES             Location/Qualifiers
     source          1..9721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             502..1209
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      757..766
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1216..1236
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    1286..1838
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1430..1447)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1657..1676
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      1859..1868
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1865..2263
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3106..3339
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3411..3532
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3448..3467)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3502..3521
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3572..3707
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3727..3746)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3728..3746)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3756..3773)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3756..3772)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3765..3787)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3914..4369
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4001..4020)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4211..4232
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4395..4499
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4500..4568,4569..5360)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4718..4737)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5531..6119
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6020..6039
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6273..6290
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6407..6428
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6443..6448,6449..6466,6467..6473)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6481..6497
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6486..6508
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6505..6521
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6505..6521
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7878..7898
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7880..7898
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7926..8152
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8153..8333
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8380..8505
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8998..9231
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9416..9460
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
       61 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttat
      121 cgatcggtac ccctagggtg aaaaggcagg tgcaaagcaa atcctatccc agcttctagc
      181 taggtgaaaa ggcaggtgca aagcaaatcc tatcccagct tctagctagg gtgaaaaggc
      241 aggtgcaaag caaatcctat cccagcttct agctaggtga aaaggcaggt gcaaagcaaa
      301 tcctatccca gcttctagct agcggcgcgc cggatccgga ggggacaagt ggagggaaag
      361 tggggccggg cggggggtgc ctgggaagcc aggctgcgct gacgtcactg ggcgcgcaat
      421 tcgggctgga gcgctttaaa aaacgagcgt gcaagcagag aggctgctcc acaccgctca
      481 ggccgcggtt taaacatcga tatggtgagc aagggcgagg aggataacat ggcctctctc
      541 ccagcgacac atgagttaca catctttggc tccatcaacg gtgtggactt tgacatggtg
      601 ggtcagggca ccggcaatcc aaatgatggt tatgaggagt taaacctgaa gtccaccaag
      661 ggtgacctcc agttctcccc ctggattctg gtccctcata tcgggtatgg cttccatcag
      721 tacctgccct accctgacgg gatgtcgcct ttccaggccg ccatggtaga tggctccgga
      781 taccaagtcc atcgcacaat gcagtttgaa gatggtgcct cccttactgt taactaccgc
      841 tacacctacg agggaagcca catcaaagga gaggcccagg tgaaggggac tggtttccct
      901 gctgacggtc ctgtgatgac caactcgctg accgctgcgg actggtgcag gtcgaagaag
      961 acttacccca acgacaaaac catcatcagt acctttaagt ggagttacac cactggaaat
     1021 ggcaagcgct accggagcac tgcgcggacc acctacacct ttgccaagcc aatggcggct
     1081 aactatctga agaaccagcc gatgtacgtg ttccgtaaga cggagctcaa gcactccaag
     1141 accgagctca acttcaagga gtggcaaaag gcctttaccg atgtgatggg catggacgag
     1201 ctgtacaagt ccaggccaaa aaagaaaaga aaggtcatcg attagttaat taatggcccg
     1261 ggaattccgc ccctctcccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     1321 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     1381 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     1441 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     1501 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     1561 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     1621 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     1681 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     1741 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     1801 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gatatggcca caacggtcgc
     1861 caccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac
     1921 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga
     1981 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact
     2041 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat
     2101 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga
     2161 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg
     2221 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taaatgcatg tattcaatct
     2281 aagcaggctt tcactttctc gccaacttac aaggcctttc tgtgtaaaca atacctgaac
     2341 ctttaccccg ttgcccggca acggccaggt ctgtgccaag tgtttgctga cgcaaccccc
     2401 actggctggg gcttggtcat gggccatcag cgcatgcgtg gaaccttttc ggctcctctg
     2461 ccgatccata ctgcggaact cctagccgct tgttttgctc gcagcaggtc tggagcaaac
     2521 attatcggga ctgataactc tgttgtccta tcccgcaaat atacatcgtt tccatggctg
     2581 ctaggctgtg ctgccaactg gatcctgcgc gggacgtcct ttgtttacgt cccgtcggcg
     2641 ctgaatcctg cggacgaccc ttctcggggt cgcttgggac tctctcgtcc ccttctccgt
     2701 ctgccgttcc gaccgaccac ggggcgcacc tctctttacg cggactcccc gtctgtgcct
     2761 tctcatctgc cggaccgtgt gcacttcgct tcacctctgc acgtcgcatg gagaccaccg
     2821 tgaacgccca ccaaatattg cccaaggtct tacataagag gactcttgga ctctcagcaa
     2881 tgtcaacgac cgaccttgag gcatacttca aagactgttt gtttaaagac tgggaggagt
     2941 tgggggagga gattaggtta aaggtctttg tactaggagg ctgtaggcat aaattggtct
     3001 gcgcaccagc accatggcgc aatcactaga gcggggtacc tttaagacca atgacttaca
     3061 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     3121 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     3181 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     3241 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     3301 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     3361 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     3421 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3481 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     3541 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3601 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3661 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     3721 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     3781 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     3841 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     3901 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3961 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4021 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4081 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4141 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4201 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4261 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4321 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     4381 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4441 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4501 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4561 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4621 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4681 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4741 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4801 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4861 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     4921 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     4981 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5041 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5101 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5161 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5221 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5281 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5341 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     5401 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5461 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5521 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5581 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5641 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5701 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5761 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5821 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5881 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5941 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6001 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6061 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6121 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6181 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6241 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6301 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     6361 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     6421 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     6481 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgct
     6541 gatgaatccc ctaatgattt tggtaaaaat cattaagtta aggtggatac acatcttgtc
     6601 atatgatcaa atggtttcgc gaaaaatcaa taatcagaca acaagatgtg cgaactcgat
     6661 attttacacg actctcttta ccaattctgc cccgaattac acttaaaacg actcaacagc
     6721 ttaacgttgg cttgccacgc attacttgac tgtaaaactc tcactcttac cgaacttggc
     6781 cgtaacctgc caaccaaagc gagaacaaaa cataacatca aacgaatcga ccgattgtta
     6841 ggtaatcgtc acctccacaa agagcgactc gctgtatacc gttggcatgc tagctttatc
     6901 tgttcgggca atacgatgcc cattgtactt gttgactggt ctgatattcg tgagcaaaaa
     6961 cgacttatgg tattgcgagc ttcagtcgca ctacacggtc gttctgttac tctttatgag
     7021 aaagcgttcc cgctttcaga gcaatgttca aagaaagctc atgaccaatt tctagccgac
     7081 cttgcgagca ttctaccgag taacaccaca ccgctcattg tcagtgatgc tggctttaaa
     7141 gtgccatggt ataaatccgt tgagaagctg ggttggtact ggttaagtcg agtaagagga
     7201 aaagtacaat atgcagacct aggagcggaa aactggaaac ctatcagcaa cttacatgat
     7261 atgtcatcta gtcactcaaa gactttaggc tataagaggc tgactaaaag caatccaatc
     7321 tcatgccaaa ttctattgta taaatctcgc tctaaaggcc gaaaaaatca gcgctcgaca
     7381 cggactcatt gtcaccaccc gtcacctaaa atctactcag cgtcggcaaa ggagccatgg
     7441 gttctagcaa ctaacttacc tgttgaaatt cgaacaccca aacaacttgt taatatctat
     7501 tcgaagcgaa tgcagattga agaaaccttc cgagacttga aaagtcctgc ctacggacta
     7561 ggcctacgcc atagccgaac gagcagctca gagcgttttg atatcatgct gctaatcgcc
     7621 ctgatgcttc aactaacatg ttggcttgcg ggcgttcatg ctcagaaaca aggttgggac
     7681 aagcacttcc aggctaacac agtcagaaat cgaaacgtac tctcaacagt tcgcttaggc
     7741 atggaagttt tgcggcattc tggctacaca ataacaaggg aagacttact cgtggctgca
     7801 accctactag ctcaaaattt attcacacat ggttacgctt tggggaaatt atgaggggat
     7861 ctctcagcgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     7921 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     7981 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     8041 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     8101 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     8161 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8221 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8281 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     8341 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     8401 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     8461 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     8521 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     8581 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     8641 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     8701 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     8761 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     8821 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     8881 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     8941 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     9001 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     9061 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     9121 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     9181 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     9241 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     9301 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     9361 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     9421 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     9481 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     9541 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     9601 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     9661 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     9721 g
//
