LOCUS       sequence-312909-        9709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for intestinal stem cells through ASCL2 
            transcriptional activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9709)
  AUTHORS   Heinz MC, Oost KC, Snippert HJG
  TITLE     Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal 
            Organoids.
  JOURNAL   STAR Protoc. 2020 Oct 8;1(3):100126. doi: 
            10.1016/j.xpro.2020.100126. eCollection 2020 Dec 18.
  PUBMED    33377020
REFERENCE   2  (bases 1 to 9709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2020-10-8"; volume: "1(3):100126. doi"; pages: " 
            10.1016/j.xpro.2020.100126. eCollection 2020 Dec 18"
COMMENT     Sequence Label: pLV 4xSTAR-mScarletI-NLS-blast
FEATURES             Location/Qualifiers
     source          1..9709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        482..586
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(587..655,656..1447)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(805..824)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1618..2206
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2107..2126
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2360..2377
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2494..2515
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2530..2535,2536..2553,2554..2560)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2568..2584
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2573..2595
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2592..2608
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2592..2608
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3965..3985
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3967..3985
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4013..4239
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4240..4420
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4467..4592
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5085..5318
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5503..5547
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5809..5926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             6310..7005
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(6443..6461)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6711..6730
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7012..7032
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    7082..7634
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7226..7243)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7453..7472
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      7655..7664
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7661..8059
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             8902..9135
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9207..9328
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9244..9263)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9298..9317
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9368..9503
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9523..9542)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9524..9542)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9552..9569)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9552..9568)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9561..9583)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
      481 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      541 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      601 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      661 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      721 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      781 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
      841 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      901 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
      961 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     1021 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     1081 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     1141 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     1201 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     1261 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     1321 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     1381 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     1441 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1501 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1561 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1621 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1681 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1741 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1801 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1861 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1921 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1981 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2041 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2101 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2161 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2221 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2281 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2341 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     2401 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     2461 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     2521 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     2581 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgctgat gaatccccta
     2641 atgattttgg taaaaatcat taagttaagg tggatacaca tcttgtcata tgatcaaatg
     2701 gtttcgcgaa aaatcaataa tcagacaaca agatgtgcga actcgatatt ttacacgact
     2761 ctctttacca attctgcccc gaattacact taaaacgact caacagctta acgttggctt
     2821 gccacgcatt acttgactgt aaaactctca ctcttaccga acttggccgt aacctgccaa
     2881 ccaaagcgag aacaaaacat aacatcaaac gaatcgaccg attgttaggt aatcgtcacc
     2941 tccacaaaga gcgactcgct gtataccgtt ggcatgctag ctttatctgt tcgggcaata
     3001 cgatgcccat tgtacttgtt gactggtctg atattcgtga gcaaaaacga cttatggtat
     3061 tgcgagcttc agtcgcacta cacggtcgtt ctgttactct ttatgagaaa gcgttcccgc
     3121 tttcagagca atgttcaaag aaagctcatg accaatttct agccgacctt gcgagcattc
     3181 taccgagtaa caccacaccg ctcattgtca gtgatgctgg ctttaaagtg ccatggtata
     3241 aatccgttga gaagctgggt tggtactggt taagtcgagt aagaggaaaa gtacaatatg
     3301 cagacctagg agcggaaaac tggaaaccta tcagcaactt acatgatatg tcatctagtc
     3361 actcaaagac tttaggctat aagaggctga ctaaaagcaa tccaatctca tgccaaattc
     3421 tattgtataa atctcgctct aaaggccgaa aaaatcagcg ctcgacacgg actcattgtc
     3481 accacccgtc acctaaaatc tactcagcgt cggcaaagga gccatgggtt ctagcaacta
     3541 acttacctgt tgaaattcga acacccaaac aacttgttaa tatctattcg aagcgaatgc
     3601 agattgaaga aaccttccga gacttgaaaa gtcctgccta cggactaggc ctacgccata
     3661 gccgaacgag cagctcagag cgttttgata tcatgctgct aatcgccctg atgcttcaac
     3721 taacatgttg gcttgcgggc gttcatgctc agaaacaagg ttgggacaag cacttccagg
     3781 ctaacacagt cagaaatcga aacgtactct caacagttcg cttaggcatg gaagttttgc
     3841 ggcattctgg ctacacaata acaagggaag acttactcgt ggctgcaacc ctactagctc
     3901 aaaatttatt cacacatggt tacgctttgg ggaaattatg aggggatctc tcagcgccaa
     3961 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
     4021 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
     4081 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
     4141 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
     4201 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
     4261 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     4321 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     4381 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     4441 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     4501 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     4561 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     4621 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     4681 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     4741 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     4801 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     4861 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     4921 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     4981 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     5041 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     5101 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     5161 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     5221 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     5281 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     5341 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     5401 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     5461 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     5521 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     5581 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     5641 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     5701 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     5761 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt ttaaaagaaa
     5821 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     5881 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg atcggtaccc
     5941 ctagggtgaa aaggcaggtg caaagcaaat cctatcccag cttctagcta ggtgaaaagg
     6001 caggtgcaaa gcaaatccta tcccagcttc tagctagggt gaaaaggcag gtgcaaagca
     6061 aatcctatcc cagcttctag ctaggtgaaa aggcaggtgc aaagcaaatc ctatcccagc
     6121 ttctagctag cggcgcgccg gatccggagg ggacaagtgg agggaaagtg gggccgggcg
     6181 gggggtgcct gggaagccag gctgcgctga cgtcactggg cgcgcaattc gggctggagc
     6241 gctttaaaaa acgagcgtgc aagcagagag gctgctccac accgctcagg ccgcggttta
     6301 aacatcgata tggtgagcaa gggcgaggca gtgatcaagg agttcatgcg gttcaaggtg
     6361 cacatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     6421 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     6481 tcctgggaca tcctgtcccc tcagttcatg tacggctcca gggccttcat caagcacccc
     6541 gccgacatcc ccgactacta taagcagtcc ttccccgagg gcttcaagtg ggagcgcgtg
     6601 atgaacttcg aggacggcgg cgccgtgacc gtgacccagg acacctccct ggaggacggc
     6661 accctgatct acaaggtgaa gctccgcggc accaacttcc ctcctgacgg ccccgtaatg
     6721 cagaagaaga caatgggctg ggaagcgtcc accgagcggt tgtaccccga ggacggcgtg
     6781 ctgaagggcg acattaagat ggccctgcgc ctgaaggacg gcggacgcta cctggcggac
     6841 ttcaagacca cctacaaggc caagaagccc gtgcagatgc ccggcgccta caacgtggac
     6901 cgcaagttgg acatcacctc ccacaacgag gactacaccg tggtggaaca gtacgaacgc
     6961 tccgagggcc gccactccac cggcggcatg gacgagctgt acaagtccag gccaaaaaag
     7021 aaaagaaagg tcatcgatta gttaattaat ggcccgggaa ttccgcccct ctccccccct
     7081 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     7141 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     7201 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     7261 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     7321 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     7381 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     7441 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     7501 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     7561 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     7621 acacgatgat aatatggata tggccacaac ggtcgccacc atggccaagc ctttgtctca
     7681 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga
     7741 agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa
     7801 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc
     7861 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt
     7921 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat
     7981 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg
     8041 ttatgtgtgg gagggctaaa tgcatgtatt caatctaagc aggctttcac tttctcgcca
     8101 acttacaagg cctttctgtg taaacaatac ctgaaccttt accccgttgc ccggcaacgg
     8161 ccaggtctgt gccaagtgtt tgctgacgca acccccactg gctggggctt ggtcatgggc
     8221 catcagcgca tgcgtggaac cttttcggct cctctgccga tccatactgc ggaactccta
     8281 gccgcttgtt ttgctcgcag caggtctgga gcaaacatta tcgggactga taactctgtt
     8341 gtcctatccc gcaaatatac atcgtttcca tggctgctag gctgtgctgc caactggatc
     8401 ctgcgcggga cgtcctttgt ttacgtcccg tcggcgctga atcctgcgga cgacccttct
     8461 cggggtcgct tgggactctc tcgtcccctt ctccgtctgc cgttccgacc gaccacgggg
     8521 cgcacctctc tttacgcgga ctccccgtct gtgccttctc atctgccgga ccgtgtgcac
     8581 ttcgcttcac ctctgcacgt cgcatggaga ccaccgtgaa cgcccaccaa atattgccca
     8641 aggtcttaca taagaggact cttggactct cagcaatgtc aacgaccgac cttgaggcat
     8701 acttcaaaga ctgtttgttt aaagactggg aggagttggg ggaggagatt aggttaaagg
     8761 tctttgtact aggaggctgt aggcataaat tggtctgcgc accagcacca tggcgcaatc
     8821 actagagcgg ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     8881 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     8941 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     9001 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     9061 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     9121 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     9181 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     9241 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9301 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     9361 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     9421 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     9481 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     9541 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     9601 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     9661 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggg
//
