LOCUS       sequence-31291-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HUWE1
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..83
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacca tgcatcatca tcatcatcac agcagcggca
       61 gagaaaactt gtatttccag ggcgagcgtt tagatgaggg gctccggaaa gaagacatgg
      121 ctgtgcatgt ccgtcgtgac catgtgtttg aagactccta tcgtgagctg catcgcaaat
      181 cccccgaaga aatgaagaat cgattgtata tagtatttga aggagaagaa gggcaggatg
      241 ctggcgggct cctgcgggag tggtatatga tcatctctcg agagatgttt aaccctatgt
      301 atgccttgtt ccgtacctca cctggtgatc gagtcaccta caccatcaat ccatcttccc
      361 actgcaaccc caaccacctc agctacttca agtttgtcgg acgcattgtg gccaaagctg
      421 tatatgacaa ccgtcttctg gagtgctact ttactcgatc cttttacaaa cacatcttgg
      481 gcaagtcagt cagatataca gatatggaga gtgaagatta ccacttctac caaggtctgg
      541 tttatctgct ggaaaatgat gtctccacac taggctatga cctcaccttc agcactgagg
      601 tccaagagtt tggagtttgt gaagttcgtg acctcaaacc caatggggcc aacatcttgg
      661 taacagagga gaataagaag gagtatgtac acctggtatg ccagatgaga atgacaggag
      721 ccatccgcaa gcagttggcg gctttcttag aaggcttcta tgagatcatt ccaaagcgcc
      781 tcatttccat cttcactgag caggagttag agctgcttat atcaggactg cccaccattg
      841 acatcgatga tctgaaatcc aacactgaat accacaagta ccagtccaac tctattcaga
//
