LOCUS       sequence-312917-        8688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven 
            human PDL1/CD274 (cloned between AgeI and PacI). Targeted cells will
            be puromycin resistant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8688)
  AUTHORS   Ma H, Jeppesen JF, Jaenisch R
  TITLE     Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into
            Mechanisms of Human CD19-Positive beta Cell Destruction.
  JOURNAL   Cell Rep Med. 2020 Sep 22;1(6):100097. doi: 
            10.1016/j.xcrm.2020.100097. eCollection 2020 Sep 22.
  PUBMED    33205073
REFERENCE   2  (bases 1 to 8688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Med."; date: "2020-09-22"; volume: "1(6):100097. doi"; pages: " 
            10.1016/j.xcrm.2020.100097. eCollection 2020 Sep 22"
COMMENT     Sequence Label: AAVS1-PDL1
FEATURES             Location/Qualifiers
     source          1..8688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(2720..2739)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2785..2840
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2839..2858)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3201..3217)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3225..3241
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3249..3255,3256..3273,3274..3279))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3294..3315
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    3389..4225
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     regulatory      4241..4250
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4250..4266)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4274..4290
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4298..4304,4305..4322,4323..4328))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4343..4364
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4481..4498)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4652..5240)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4732..4751)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5411..6202,6203..6271))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6034..6053
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6272..6376)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6444..6462
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6500..6522)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6622..6641
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6835..6857
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6849..6866
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6850..6866
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6900..7703
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    7710..7735
                     /label=SA
                     /note="splice acceptor site"
     CDS             7759..7812
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7822..8421
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7822..7841)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8318..8338
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(8453..8470)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8459..8683
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcgc crggsggggs ggggsggggs grggggsggg
      541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaccgg tccaaccatg
     1741 aggatatttg ctgtctttat attcatgacc tactggcatt tgctgaacgc atttactgtc
     1801 acggttccca aggacctata tgtggtagag tatggtagca atatgacaat tgaatgcaaa
     1861 ttcccagtag aaaaacaatt agacctggct gcactaattg tctattggga aatggaggat
     1921 aagaacatta ttcaatttgt gcatggagag gaagacctga aggttcagca tagtagctac
     1981 agacagaggg cccggctgtt gaaggaccag ctctccctgg gaaatgctgc acttcagatc
     2041 acagatgtga aattgcagga tgcaggggtg taccgctgca tgatcagcta tggtggtgcc
     2101 gactacaagc gaattactgt gaaagtcaat gccccataca acaaaatcaa ccaaagaatt
     2161 ttggttgtgg atccagtcac ctctgaacat gaactgacat gtcaggctga gggctacccc
     2221 aaggccgaag tcatctggac aagcagtgac catcaagtcc tgagtggtaa gaccaccacc
     2281 accaattcca agagagagga gaagcttttc aatgtgacca gcacactgag aatcaacaca
     2341 acaactaatg agattttcta ctgcactttt aggagattag atcctgagga aaaccataca
     2401 gctgaattgg tcatcccaga actacctctg gcacatcctc caaatgaaag gactcacttg
     2461 gtaattctgg gagccatctt attatgcctt ggtgtagcac tgacattcat cttccgttta
     2521 agaaaaggga gaatgatgga tgtgaaaaaa tgtggcatcc aagatacaaa ctcaaagaag
     2581 caaagtgata cacatttgga ggagacgtaa ttaattaaga tatcgtcgac acgcgtgaat
     2641 tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg
     2701 ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
     2761 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     2821 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
     2881 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca
     2941 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat
     3001 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt
     3061 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct
     3121 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc
     3181 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     3241 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     3301 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     3361 tgccagcgga tcgacagtac taagctttac tagggacagg attggtgaca gaaaagcccc
     3421 atccttaggc ctcctccttc ctagtctcct gatattgggt ctaaccccca cctcctgtta
     3481 ggcagattcc ttatctggtg acacaccccc atttcctgga gccatctctc tccttgccag
     3541 aacctctaag gtttgcttac gatggagcca gagaggatcc tgggagggag agcttggcag
     3601 ggggtgggag ggaagggggg gatgcgtgac ctgcccggtt ctcagtggcc accctgcgct
     3661 accctctccc agaacctgag ctgctctgac gcggctgtct ggtgcgtttc actgatcctg
     3721 gtgctgcagc ttccttacac ttcccaagag gagaagcagt ttggaaaaac aaaatcagaa
     3781 taagttggtc ctgagttcta actttggctc ttcacctttc tagtccccaa tttatattgt
     3841 tcctccgtgc gtcagtttta cctgtgagat aaggccagta gccagccccg tcctggcagg
     3901 gctgtggtga ggaggggggt gtccgtgtgg aaaactccct ttgtgagaat ggtgcgtcct
     3961 aggtgttcac caggtcgtgg ccgcctctac tccctttctc tttctccatc cttctttcct
     4021 taaagagtcc ccagtgctat ctgggacata ttcctccgcc cagagcaggg tcccgcttcc
     4081 ctaaggccct gctctgggct tctgggtttg agtccttggc aagcccagga gaggcgctca
     4141 ggcttccctg tcccccttcc tcgtccacca tctcatgccc ctggctctcc tgccccttcc
     4201 ctacaggggt tcctggctct gctctaaggg cgaattcgcg gccgccatgg tcatagctgt
     4261 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4321 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     4381 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     4441 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     4501 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     4561 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     4621 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4681 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4741 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4801 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4861 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4921 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4981 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5041 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     5101 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5161 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5221 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5281 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     5341 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     5401 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     5461 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     5521 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     5581 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5641 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5701 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5761 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5821 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5881 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5941 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6001 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     6061 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     6121 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     6181 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6241 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6301 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     6361 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     6421 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     6481 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     6541 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     6601 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     6661 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
     6721 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     6781 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     6841 cacgacgttg taaaacgacg gccagtgaat tccctgcagg tttaaacgaa ttcgcccttt
     6901 gctttctctg accagcattc tctcccctgg gcctgtgccg ctttctgtct gcagcttgtg
     6961 gcctgggtca cctctacggc tggcccagat ccttccctgc cgcctccttc aggttccgtc
     7021 ttcctccact ccctcttccc cttgctctct gctgtgttgc tgcccaagga tgctctttcc
     7081 ggagcacttc cttctcggcg ctgcaccacg tgatgtcctc tgagcggatc ctccccgtgt
     7141 ctgggtcctc tccgggcatc tctcctccct cacccaaccc catgccgtct tcactcgctg
     7201 ggttcccttt tccttctcct tctggggcct gtgccatctc tcgtttctta ggatggcctt
     7261 ctccgacgga tgtctccctt gcgtcccgcc tccccttctt gtaggcctgc atcatcaccg
     7321 tttttctgga caaccccaaa gtaccccgtc tccctggctt tagccacctc tccatcctct
     7381 tgctttcttt gcctggacac cccgttctcc tgtggattcg ggtcacctct cactcctttc
     7441 atttgggcag ctcccctacc ccccttacct ctctagtctg tgctagctct tccagccccc
     7501 tgtcatggca tcttccaggg gtccgagagc tcagctagtc ttcttcctcc aacccgggcc
     7561 cctatgtcca cttcaggaca gcatgtttgc tgcctccagg gatcctgtgt ccccgagctg
     7621 ggaccacctt atattcccag ggccggttaa tgtggctctg gttctgggta cttttatctg
     7681 tcccctccac cccacagtgg ggcaagcttc tgacctcttc tcttcctccc acagggcctc
     7741 gagagatctg gcagcggaga gggcagagga agtcttctaa catgcggtga cgtggaggag
     7801 aatcccggcc ctaggctcga gatgaccgag tacaagccca cggtgcgcct cgccacccgc
     7861 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     7921 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     7981 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     8041 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     8101 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     8161 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     8221 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     8281 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     8341 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     8401 acccgcaagc ccggtgcctg atctagaggg cccgtttaaa cccgctgatc agcctcgact
     8461 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     8521 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     8581 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     8641 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tgggtctc
//
