LOCUS       sequence-312918-        8019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Light-controlled activation of expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8019)
  AUTHORS   Redchuk TA, Omelina ES, Chernov KG, Verkhusha VV
  TITLE     Near-infrared optogenetic pair for protein regulation and spectral 
            multiplexing.
  JOURNAL   Nat Chem Biol. 2017 Mar 27. doi: 10.1038/nchembio.2343.
  PUBMED    28346403
REFERENCE   2  (bases 1 to 8019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2017 Mar 27. doi: 10.1038/nchembio.2343."
COMMENT     Sequence Label: pQP-T2A
FEATURES             Location/Qualifiers
     source          1..8019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   9..138
                     /label=AAV2 ITR
     enhancer        153..456
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        457..660
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     611..631
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      684..693
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3074..3307
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3317..3370
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3407..3844
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             4301..4315
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     rep_origin      4807..5320
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4894..4913)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5104..5125
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5802..5821)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5921..5943
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5981..5999)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6067..6171
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6172..6240,6241..7032)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6390..6409)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7203..7791
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7692..7711
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatgcagct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg
       61 cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact
      121 ccatcactag gggttccttg tagttaccta ggcgttacat aacttacggt aaatggcccg
      181 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      241 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      301 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      361 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      421 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      481 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      541 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      601 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      661 cgtttagtga accgtcaagc ttaccatggt ggcaggtcat gcctctggca gccccgcatt
      721 cgggaccgcc gatctttcga attgcgaacg tgaagagatc cacctcgccg gctcgatcca
      781 gccgcatggc gcgcttctgg tcgtcagcga gccggatcat cgcatcatcc aggccagcgc
      841 caacgccgcg gaatttctga atctcggaag cgtgctcggc gttccgctcg ccgagatcga
      901 cggcgatctg ttgatcaaga tcctgccgca tctcgatccc accgccgaag gcatgccggt
      961 cgcggtgcgc tgccggatcg gcaatccctc cacggagtac gacggtctga tgcatcggcc
     1021 tccggaaggc gggctgatca tcgagctcga acgtgccggc ccgccgatcg atctgtccgg
     1081 cacgctggcg ccggcgctgg agcggatccg cacggcgggc tcgctgcgcg cgctgtgcga
     1141 tgacaccgcg ctgctgtttc agcagtgcac cggctacgac cgggtgatgg tgtatcgctt
     1201 cgacgagcag ggccacggcg aagtgttctc cgagcgccac gtgcccgggc tcgaatccta
     1261 tttcggcaac cgctatccgt cgtcggacat tccgcagatg gcgcggcggc tgtacgagcg
     1321 gcagcgcgtc cgcgtgctgg tcgacgtcag ctatcagccg gtgccgctgg agccgcggct
     1381 gtcgccgctg accgggcgcg atctcgacat gtcgggctgc ttcctgcgct cgatgtcgcc
     1441 gatccatctg cagtacctga agaacatggg cgtgcgcgcc accctggtgg tgtcgctggt
     1501 ggtcggcggc aagctgtggg gcctggttgc ctgtcaccat tatctgccgc gcttcatcca
     1561 tttcgagctg cgggcgatct gcgaactgct cgccgaagcg atcgcgacgc ggatcaccgc
     1621 gcttgagagc ttcgcgcaga gccagtcgga gctgttcgtg cagcggctcg aacagcgcat
     1681 gatcgaagcg atcacccgtg aaggcgattg gcgcgcagcg attttcgaca ccagccaatc
     1741 gatcctgcag ccgctgcacg ccgacggttg cgcgctggtg tacgaagacc agatcaggac
     1801 catcggtgac gtaccttcca cgcaggatgt tcgcgagatc gccgggtggc tcgatcgcca
     1861 gccacgtgcg gcggtgacct cgaccgcgtc gctcggtctc gacgtgccgg agctcgcgca
     1921 tctgacgcgg atggcgagcg gcgtggtcgc ggcgccgatt tcggatcatc gcggcgagtt
     1981 tctgatgtgg ttccgccccg agcgcgtcca caccgttacc tggggcggcg atccgaagaa
     2041 gccgttcacg atgggcgata caccggcgga tctgtcgccg cggcgctcct tcgccaaatg
     2101 gcatcaggtt gtcgaaggca cgtccgatcc gtggacggcc gccgatctcg ccgcggctcg
     2161 caccatcggt cagaccgtcg ccgacatcgt gctgcaattc cgcgcggtgc ggacactgat
     2221 cgcccgcgaa cagtacgaac agttttcgtc ccaggtgcac gcttcgatgc agccggtgct
     2281 gatcaccgac gccgaaggcc gcatcctgct gatgaacgac tcgttccgcg acatgttgcc
     2341 ggcggggtcg ccatccgccg tccatctcga cgatctcgcc gggttcttcg tcgaatcgaa
     2401 cgatttcctg cgcaacgtcg ccgaactgat cgatcacggc cgcgggtggc gcggcgaagt
     2461 tctgctgcgc ggcgcaggta atcgcccgtt gccgctggca gtgcgcgccg atccggtgac
     2521 gcgcacggag gaccagtcgc tcggcttcgt gctgatcttc agcgacgcta ccgatcgtcg
     2581 caccgcagat gccgcacgca cgcgtttcca ggaaggcatt cttgccagcg cacgtcccgg
     2641 cgtgcggctc gactccaagt ccgacctctt gcacgagaag ctgctgtccg cgctggtcga
     2701 gaacgcgcag cttgccgcat tggaaattac ttacggcgtc gagaccggac gcatcgccga
     2761 gctgctcgaa ggcgttcgcc agtcgatgct gcgcaccgcc gaagtgctcg gccatctggt
     2821 gcagcacgcg gcgcgcacgg ccggcagcga cagctcgagc aatggctcgc agaacaagaa
     2881 ggaattcgat agtgctggta gtgctggtag tgctggtact agagcgtaca gccgcgcgcg
     2941 tacgaaaaac aattacgggt ctaccatcga gggcctgctc gatctcccgg acgacgacgc
     3001 ccccgaagag gcggggctgg cggctccgcg cctgtccttt ctccccgcgg gacacacgcg
     3061 cagactgtcg acggcccccc cgaccgatgt cagcctgggg gacgagctcc acttagacgg
     3121 cgaggacgtg gcgatggcgc atgccgacgc gctagacgat ttcgatctgg acatgttggg
     3181 ggacggggat tccccgggtc cgggatttac cccccacgac tccgccccct acggcgctct
     3241 ggatatggcc gacttcgagt ttgagcagat gtttaccgat gcccttggaa ttgacgagta
     3301 cggtgggagc ggccgcgagg gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa
     3361 tcctggccca atgcccaaga agaagaggaa gggaggtggt ggaagcaagc tactgtcttc
     3421 tatcgaacaa gcatgcgata tttgccgact taaaaagctc aagtgctcca aagaaaaacc
     3481 gaagtgcgcc aagtgtctga agaacaactg ggagtgtcgc tactctccca aaaccaaaag
     3541 gtctccgctg actagggcac atctgacaga agtggaatca aggctagaaa gactggaaca
     3601 gctatttcta ctgatttttc ctcgagaaga ccttgacatg attttgaaaa tggattcttt
     3661 acaggatata aaagcattgt taacaggatt atttgtacaa gataatgtga ataaagatgc
     3721 cgtcacagat agattggctt cagtggagac tgatatgcct ctaacattga gacagcatag
     3781 aataagtgcg acatcatcat cggaagagag tagtaacaaa ggtcaaagac agttgactgt
     3841 atcgccggaa ttcggcaaga acatgcaggc ggtcaccgag ctgcattccc ggctgatcgc
     3901 tgcgcagcag gcgatggagc gcgactattg gcggttgcgt gaattggaga ctcgctaccg
     3961 cctggtgttc gacgctgccg ccgatgcggt gatgatcgtc tccgccggcg acatgcgcat
     4021 cgtcgaagcc aaccgggcgg cggtgaatgc gatcagccgc gtcgagcgcg gcaatgacga
     4081 ccttgcgggg cgtgatttcc tcgccgaagt ggcggctgcc gatcgcgatg cggtgcgcga
     4141 catgctggcc caggtgcgtc agcgcggcac cgcactcagc gtcctcgttc atctcggccg
     4201 ttacgaccgc gcctggatgc tgcgcggttc gctgatgtcg tccgagcgtc gtcaggtttt
     4261 cctgctgcac ttcaccccgg tgaccacgac tcccgcgatc gacgacgacg acaaaggtgt
     4321 cgttgcttct gctgccgacg gtgcagaggg cgcctcagac gacgcagagg attaaagctg
     4381 caataaacaa gttaacaaca aactagtacc taggatttga gcgagcgagc gcgcagctgg
     4441 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     4501 gaatggcgat tccgttgcaa tggctggcgg taatattgtt ctggatatta ccagcaaggc
     4561 cgatagtttg agttcttcta ctcaggcaag tgatgttatt actaatcaaa gaagtattgc
     4621 gacaacggtt aatttgcgtg atggacagac tcttttactc ggtggcctca ctgattataa
     4681 aaacacttct caggattctg gcgtaccgtt cctgtctaaa atccctttaa tcggcctcct
     4741 gtttagctcc cgctctgatt ctaacgagga aagcacgtta tacgtgctcg tcaaagcaac
     4801 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4861 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4921 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4981 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     5041 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5101 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     5161 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     5221 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaaatattt gcttatacaa
     5281 tcttcctgtt tttggggctt ttctgattat caaccggggt acatatgatt gacatgctag
     5341 ttttacgatt accgttcatc gattctcttg tttgctccag actctcaggc aatgacctga
     5401 tagcctttgt agagacctct caaaaatagc taccctctcc ggcatgaatt tatcagctag
     5461 aacggttgaa tatcatattg atggtgattt gactgtctcc ggcctttctc acccgtttga
     5521 atctttacct acacattact caggcattgc atttaaaata tatgagggtt ctaaaaattt
     5581 ttatccttgc gttgaaataa aggcttctcc cgcaaaagta ttacagggtc ataatgtttt
     5641 tggtacaacc gatttagctt tatgctctga ggctttattg cttaattttg ctaattcttt
     5701 gccttgcctg tatgatttat tggatgttgg aatcgcctga tgcggtattt tctccttacg
     5761 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     5821 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     5881 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     5941 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     6001 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     6061 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6121 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6181 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6241 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     6301 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6361 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     6421 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6481 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6541 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6601 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6661 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6721 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6781 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6841 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6901 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6961 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     7021 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7081 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7141 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7201 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7261 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7321 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     7381 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7441 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7501 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7561 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7621 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7681 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7741 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7801 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7861 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7921 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     7981 atacgcaaac cgcctctccc cgcgcgttgg ccgattcat
//
