LOCUS       sequence-312925-        9970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing SHOC2 with V5 C-term tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Sulahian R, Kwon JJ, Walsh KH, Pailler E, Bosse TL, Thaker M, 
            Almanza D, Dempster JM, Pan J, Piccioni F, Dumont N, Gonzalez A, 
            Rennhack J, Nabet B, Bachman JA, Goodale A, Lee Y, Bagul M, Liao R, 
            Navarro A, Yuan TL, Ng RWS, Raghavan S, Gray NS, Tsherniak A, 
            Vazquez F, Root DE, Firestone AJ, Settleman J, Hahn WC, Aguirre AJ
  TITLE     Synthetic Lethal Interaction of SHOC2 Depletion with MEK Inhibition 
            in RAS-Driven Cancers.
  JOURNAL   Cell Rep. 2019 Oct 1;29(1):118-134.e8. doi: 
            10.1016/j.celrep.2019.08.090.
  PUBMED    31577942
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.08"; journalName: "Cell Rep"; date: 
            "2019-10-1- 1"; volume: "29"; issue: "1"; pages: "118-134.e8"
COMMENT     Sequence Label: pLX311-SHOC2-V5
FEATURES             Location/Qualifiers
     source          1..9970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2823..3033
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2845..3041
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2892..3027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3063..3458
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3532..3649
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3787..4965
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4018..4956
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4913..4933
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    4985..5009
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6756..6780)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6788..6829
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6880..7468
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6933..6953)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7351..7362)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7540..7773
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7851..7972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7888..7907)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7942..7961
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8012..8147
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8167..8186)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8168..8186)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8196..8213)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8196..8212)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8205..8227)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8354..8809
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8441..8460)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8651..8672
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8835..8939
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8940..9008,9009..9800)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9158..9177)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     2821 cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata gtcccgcccc
     2881 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     2941 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3001 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct agaggtacca
     3061 ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa
     3121 tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg
     3181 gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg
     3241 tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg
     3301 gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc
     3361 tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga
     3421 ttcgtgaatt gctgccctct ggttatgtgt gggagggcct gcagctgcag tagtaaggcg
     3481 cgccgttaac gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag
     3541 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     3601 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     3661 cagggacagc agagatccac tttggcgccg gctcgaggcc tgcaggtgca aagatggata
     3721 aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg
     3781 ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     3841 ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg
     3901 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     3961 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
     4021 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
     4081 tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     4141 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     4201 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     4261 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     4321 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     4381 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     4441 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     4501 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     4561 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     4621 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     4681 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     4741 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     4801 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     4861 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     4921 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaggcta gcatcgattg
     4981 atcaacaagt ttgtacaaaa aagttggcat gagtagtagt ttaggaaaag aaaaagactc
     5041 taaagaaaaa gatcccaaag taccatcagc caaggaaaga gaaaaggagg caaaagcctc
     5101 tggaggtttt gggaaagaga gcaaagaaaa agaacctaag accaaaggga aagatgccaa
     5161 agatggaaag aaggactcca gtgctgccca accaggggtg gcattttcag ttgacaatac
     5221 gatcaaacgg ccaaacccag cacctgggac tagaaaaaaa tccagcaatg cagaggtgat
     5281 taaagagctc aacaaatgcc gggaagagaa ttcaatgcgt ttggacttat ccaagagatc
     5341 tatacacata ttgccatcat caatcaaaga gttgactcaa ttaacagaac tttatttata
     5401 cagtaacaaa ttgcagtccc tcccagcaga ggtgggatgt ttagtaaatc tcatgacact
     5461 ggctctaagt gaaaattcac ttaccagttt gcctgactct cttgataact tgaagaagct
     5521 gcggatgctt gatttacggc ataataaact gagagaaatt ccttcagtgg tgtataggct
     5581 ggattctctc accactcttt atcttcgatt taatcgtata actactgtgg aaaaggacat
     5641 caaaaacttg tcaaaactca gcatgcttag cattcgagag aacaaaatta aacaactacc
     5701 tgctgaaatt ggtgaattat gtaatctcat cacgctggat gtagctcaca atcaacttga
     5761 acaccttcca aaggagattg gaaactgtac acagataacc aaccttgact tgcagcacaa
     5821 tgaactgcta gacctcccag atactatagg aaacctgtcc agtttaagtc gtcttggtct
     5881 gagatataac agactgtcag caatacccag atcattagca aaatgcagtg cacttgaaga
     5941 attaaattta gagaacaata acatttctac tttaccagag agtcttttat caagtcttgt
     6001 gaaactgaat agtttgacct tagctagaaa ttgcttccag ttgtatccag tgggtggtcc
     6061 atctcagttt tctaccatct attccctcaa catggaacac aatcgaatca acaaaattcc
     6121 atttggaatt ttctccagag caaaagtatt aagtaagctg aatatgaagg acaatcagtt
     6181 aacatcactt cccttggatt ttggaacttg gaccagtatg gtagaattga atttagccac
     6241 taatcagctc acaaagatcc ctgaggatgt gtctggtctc gtttctcttg aggttcttat
     6301 cttatcaaac aatcttctaa agaagcttcc ccatggtctt ggaaacctta ggaagttaag
     6361 agagttggat ctagaagaga acaaattgga atccttgcca aatgaaattg catatcttaa
     6421 ggatttacag aaattagtct tgacaaacaa ccagttgacc actcttccca gaggcattgg
     6481 tcaccttact aatctcacac atctgggcct tggagagaac ctacttactc accttcctga
     6541 agaaattggt acactggaga acctagaaga actgtatttg aatgacaacc ccaacctgca
     6601 tagccttccc tttgagctgg cactctgcag caagctttca atcatgagta ttgagaactg
     6661 tccactcagt caccttccac ctcagattgt tgctgggggg ccttctttca tcattcagtt
     6721 cttaaagatg cagggtccat atcgtgccat ggtctgccca actttcttgt acaaagtggt
     6781 tgatatcggt aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac
     6841 tagtaccggt taagtcgaca atcaacgcgt taagtcgaca atcaacctct ggattacaaa
     6901 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6961 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     7021 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     7081 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7141 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7201 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7261 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     7321 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7381 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     7441 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     7501 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7561 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     7621 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7681 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7741 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     7801 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     7861 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7921 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7981 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     8041 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     8101 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     8161 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     8221 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     8281 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     8341 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     8401 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     8461 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     8521 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     8581 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     8641 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     8701 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     8761 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     8821 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     8881 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8941 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     9001 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     9061 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     9121 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     9181 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     9241 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     9301 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     9361 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     9421 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     9481 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     9541 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     9601 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     9661 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     9721 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     9781 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     9841 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9901 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9961 aaggatcttc
//
