LOCUS       sequence-31293-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBC32
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..85
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             589..606
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(659..677)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      673..720
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(844..863)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tttgtttact ttaagaagga gatataccat gggcagcagc catcatcatc atcatcacag
       61 cagcggcctg gttccgcgtg gtagtatgtc ggggatcgcc ctcagcagac tcgcccagga
      121 gaggaaagca tggaggaaag accacccatt tggtttcgtg gctgtcccaa caaaaaatcc
      181 cgatggcacg atgaacctca tgaactggga gtgcgccatt ccaggaaaga aagggactcc
      241 gtgggaagga ggcttgttta aactacggat gcttttcaaa gatgattatc catcttcgcc
      301 accaaaatgt aaattcgaac caccattatt tcacccgaat gtgtaccctt cggggacagt
      361 gtgcctgtcc atcttagagg aggacaagga ctggaggcca gccatcacaa tcaaacagat
      421 cctattagga atacaggaac ttctaaatga accaaatatc caagacccag ctcaagcaga
      481 ggcctacacg atttactgcc aaaacagagt ggagtacgag aaaagggtcc gagcacaagc
      541 caagaagttt gcgccctcat gatgacgaag cttgcggccg cactcgagca ccaccaccac
      601 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      661 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
      721 ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa
      781 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      841 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggc
//
