LOCUS       sequence-312934-       17876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17876)
  AUTHORS   Qian F, Boletta A, Bhunia AK, Xu H, Liu L, Ahrabi AK, Watnick TJ, 
            Zhou F, Germino GG
  TITLE     Cleavage of polycystin-1 requires the receptor for egg jelly domain 
            and is disrupted by human autosomal-dominant polycystic kidney 
            disease 1-associated mutations.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Dec 24. 99(26):16981-6.
  PUBMED    12482949
REFERENCE   2  (bases 1 to 17876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2002 Dec 24. 99(26):16981-6."
COMMENT     Sequence Label: WT (AF20)
FEATURES             Location/Qualifiers
     source          1..17876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      125..713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     614..633
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        952..1331
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1332..1535
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1532..1556
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1671..1803
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1786..1805
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1848..1866
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          1897..2469
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     2477..2496
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             15640..15663
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    15723..15844
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15734..15753)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     15788..15807
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      16078..16533
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(16165..16184)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     16375..16396
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(16600..16619)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     16719..16741
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(16779..16797)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        16865..16969
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(16970..17038,17039..17830)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(17188..17207)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
       61 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
      121 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
      181 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      241 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
      301 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      361 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      421 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      481 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      541 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      601 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      661 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      721 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atggctcgac
      781 agatcgatct ggccaaaaag gccggaccgg atcttcaata ttggccatta gccatattat
      841 tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat
      901 atcataatat gtacatttat attggctcat gtccaatatg accgccatgt tggcattgat
      961 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     1021 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     1081 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     1141 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     1201 atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     1261 cccagtacat gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg
     1321 ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact
     1381 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     1441 atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta
     1501 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcacta
     1561 gaagctttat tgcggtagtt tatcacagtt aaattgctaa cgcagtcagt gcttctgaca
     1621 caacagtctc gaacttaagc tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca
     1681 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac
     1741 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca
     1801 caggtgtcca ctcccagttc aattacagct cttaaggcta gagtacttaa tacgactcac
     1861 tataggctag tctggatcga tcctgagaac ttcagggtga gtttggggac ccttgattgt
     1921 tctttctttt tcgctattgt aaaattcatg ttatatggag ggggcaaagt tttcagggtg
     1981 ttgtttagaa tgggaagatg tcccttgtat caccatggac cctcatgata attttgtttc
     2041 tttcactttc tactctgttg acaaccattg tctcctctta ttttcttttc attttctgta
     2101 actttttcgt taaactttag cttgcatttg taacgaattt ttaaattcac ttttgtttat
     2161 ttgtcagatt gtaagtactt tctctaatca cttttttttc aaggcaatca gggtatatta
     2221 tattgtactt cagcacagtt ttagagaaca attgttataa ttaaatgata aggtagaata
     2281 tttctgcata taaattctgg ctggcgtgga aatattctta ttggtagaaa caactacatc
     2341 ctggtcatca tcctgccttt ctctttatgg ttacaatgat atacactgtt tgagatgagg
     2401 ataaaatact ctgagtccaa accgggcccc tctgctaacc atgttcatgc cttcttcttt
     2461 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     2521 ttcgcggccg cgtcgacagc gtccgagcgg gcggccgagc tcccggagcg gcctggcccc
     2581 gagccccgag cgggcgtcgc tcagcagcag gtcgcggccg cagccccatc cagccccgcg
     2641 cccgccatgc cgtccgcggg ccccgcctga gctgcggcct ccgcgcgcgg gcgggcctgg
     2701 ggacggcggg gccatgcgcg cgctgcccta acgatgccgc ccgccgcgcc cgcccgcctg
     2761 gcgctggccc tgggcctggg cctgtggctc ggggcgctgg cggggggccc cgggcgcggc
     2821 tgcgggccct gcgagccccc ctgcctctgc ggcccagcgc ccggcgccgc ctgccgcgtc
     2881 aactgctcgg gccgcgggct gcggacgctc ggtcccgcgc tgcgcatccc cgcggacgcc
     2941 acagcgctag acgtctccca caacctgctc cgggcgctgg acgttgggct cctggcgaac
     3001 ctctcggcgc tggcagagct ggatataagc aacaacaaga tttctacgtt agaagaagga
     3061 atatttgcta atttatttaa tttaagtgaa ataaacctga gtgggaaccc gtttgagtgt
     3121 gactgtggcc tggcgtggct gccgcgatgg gcggaggagc agcaggtgcg ggtggtgcag
     3181 cccgaggcag ccacgtgtgc tgggcctggc tccctggctg gccagcctct gcttggcatc
     3241 cccttgctgg acagtggctg tggtgaggag tatgtcgcct gcctccctga caacagctca
     3301 ggcaccgtgg cagcagtgtc cttttcagct gcccacgaag gcctgcttca gccagaggcc
     3361 tgcagcgcct tctgcttctc caccggccag ggcctcgcag ccctctcgga gcagggctgg
     3421 tgcctgtgtg gggcggccca gccctccagt gcctcctttg cctgcctgtc cctctgctcc
     3481 ggccccccgc cacctcctgc ccccacctgt aggggcccca ccctcctcca gcacgtcttc
     3541 cctgcctccc caggggccac cctggtgggg ccccacggac ctctggcctc tggccagcta
     3601 gcagccttcc acatcgctgc cccgctccct gtcactgcca cacgctggga cttcggagac
     3661 ggctccgccg aggtggatgc cgctgggccg gctgcctcgc atcgctatgt gctgcctggg
     3721 cgctatcacg tgacggccgt gctggccctg ggggccggct cagccctgct ggggacagac
     3781 gtgcaggtgg aagcggcacc tgccgccctg gagctcgtgt gcccgtcctc ggtgcagagt
     3841 gacgagagcc ttgacctcag catccagaac cgcggtggtt caggcctgga ggccgcctac
     3901 agcatcgtgg ccctgggcga ggagccggcc cgagcggtgc acccgctctg cccctcggac
     3961 acggagatct tccctggcaa cgggcactgc taccgcctgg tggtggagaa ggcggcctgg
     4021 ctgcaggcgc aggagcagtg tcaggcctgg gccggggccg ccctggcaat ggtggacagt
     4081 cccgccgtgc agcgcttcct ggtctctcgg gtcaccagga gcctagacgt gtggatcggc
     4141 ttctcgactg tgcagggggt ggaggtgggc ccagcgccgc agggcgaggc cttcagcctg
     4201 gagagctgcc agaactggct gcccggggag ccacacccag ccacagccga gcactgcgtc
     4261 cggctcgggc ccaccgggtg gtgtaacacc gacctgtgct cagcgccgca cagctacgtc
     4321 tgcgagctgc agcccggagg cccagtgcag gatgccgaga acctcctcgt gggagcgccc
     4381 agtggggacc tgcagggacc cctgacgcct ctggcacagc aggacggcct ctcagccccg
     4441 cacgagcccg tggaggtcat ggtattcccg ggcctgcgtc tgagccgtga agccttcctc
     4501 accacggccg aatttgggac ccaggagctc cggcggcccg cccagctgcg gctgcaggtg
     4561 taccggctcc tcagcacagc agggaccccg gagaacggca gcgagcctga gagcaggtcc
     4621 ccggacaaca ggacccagct ggcccccgcg tgcatgccag ggggacgctg gtgccctgga
     4681 gccaacatct gcttgccgct ggacgcctcc tgccaccccc aggcctgcgc caatggctgc
     4741 acgtcagggc cagggctacc cggggccccc tatgcgctat ggagagagtt cctcttctcc
     4801 gttcccgcgg ggccccccgc gcagtactcg gtcaccctcc acggccagga tgtcctcatg
     4861 ctccctggtg acctcgttgg cttgcagcac gacgctggcc ctggcgccct cctgcactgc
     4921 tcgccggctc ccggccaccc tggtccccag gccccgtacc tctccgccaa cgcctcgtca
     4981 tggctgcccc acttgccagc ccagctggag ggcacttggg cctgccctgc ctgtgccctg
     5041 cggctgcttg cagccacgga acagctcacc gtgctgctgg gcttgaggcc caaccctgga
     5101 ctgcggctgc ctgggcgcta tgaggtccgg gcagaggtgg gcaatggcgt gtccaggcac
     5161 aacctctcct gcagctttga cgtggtctcc ccagtggctg ggctgcgggt catctaccct
     5221 gccccccgcg acggccgcct ctacgtgccc accaacggct cagccttggt gctccaggtg
     5281 gactctggtg ccaacgccac ggccacggct cgctggcctg ggggcagtgt cagcgcccgc
     5341 tttgagaatg tctgccctgc cctggtggcc accttcgtgc ccggctgccc ctgggagacc
     5401 aacgataccc tgttctcagt ggtagcactg ccgtggctca gtgaggggga gcacgtggtg
     5461 gacgtggtgg tggaaaacag cgccagccgg gccaacctca gcctgcgggt gacggcggag
     5521 gagcccatct gtggcctccg cgccacgccc agccccgagg cccgtgtact gcagggagtc
     5581 ctagtgaggt acagccccgt ggtggaggcc ggctcggaca tggtcttccg gtggaccatc
     5641 aacgacaagc agtccctgac cttccagaac gtggtcttca atgtcattta tcagagcgcg
     5701 gcggtcttca agctctcact gacggccccc aaccacgtga gcaacgtcac cgtgaactac
     5761 aacgtaaccg tggagcggat gaacaggatg cagggtctgc aggtctccac agtgccggcc
     5821 gtgctgtccc ccaatgccac gctggcactg acggcgggcg tgctggtgga ctcggccgtg
     5881 gaggtggcct tcctgtggac ctttggggat ggggagcagg ccctccacca gttccagcct
     5941 ccgtacaacg agtccttccc ggttccagac ccctcggtgg cccaggtgct ggtggagcac
     6001 aatgtcatgc acacctacgc tgccccaggt gagtacctcc tgaccgtgct ggcatctaat
     6061 gccttcgaga acctgacgca gcaggtgcct gtgagcgtgc gcgcctccct gccctccgtg
     6121 gctgtgggtg tgagtgacgg cgtcctggtg gccggccggc ccgtcacctt ctacccgcac
     6181 ccgctgccct cgcctggggg tgttctttac acgtgggact tcggggacgg ctcccctgtc
     6241 ctgacccaga gccagccggc tgccaaccac acctatgcct cgaggggcac ctaccacgtg
     6301 cgcctggagg tcaacaacac ggtgagcggt gcggcggccc aggcggatgt gcgcgtcttt
     6361 gaggagctcc gcggactcag cgtggacatg agcctggccg tggagcaggg cgcccccgtg
     6421 gtggtcagcg ccgcggtgca gacgggcgac aacatcacgt ggaccttcga catgggggac
     6481 ggcaccgtgc tgtcgggccc ggaggcaaca gtggagcatg tgtacctgcg ggcacagaac
     6541 tgcacagtga ccgtgggtgc ggccagcccc gccggccacc tggcccggag cctgcacgtg
     6601 ctggtcttcg tcctggaggt gctgcgcgtt gaacccgccg cctgcatccc cacgcagcct
     6661 gacgcgcggc tcacggccta cgtcaccggg aacccggccc actacctctt cgactggacc
     6721 ttcggggatg gctcctccaa cacgaccgtg cgggggtgcc cgacggtgac acacaacttc
     6781 acgcggagcg gcacgttccc cctggcgctg gtgctgtcca gccgcgtgaa cagggcgcat
     6841 tacttcacca gcatctgcgt ggagccagag gtgggcaacg tcaccctgca gccagagagg
     6901 cagtttgtgc agctcgggga cgaggcctgg ctggtggcat gtgcctggcc cccgttcccc
     6961 taccgctaca cctgggactt tggcaccgag gaagccgccc ccacccgtgc caggggccct
     7021 gaggtgacgt tcatctaccg agacccaggc tcctatcttg tgacagtcac cgcgtccaac
     7081 aacatctctg ctgccaatga ctcagccctg gtggaggtgc aggagcccgt gctggtcacc
     7141 agcatcaagg tcaatggctc ccttgggctg gagctgcagc agccgtacct gttctctgct
     7201 gtgggccgtg ggcgccccgc cagctacctg tgggatctgg gggacggtgg gtggctcgag
     7261 ggtccggagg tcacccacgc ttacaacagc acaggtgact tcaccgttag ggtggccggc
     7321 tggaatgagg tgagccgcag cgaggcctgg ctcaatgtga cggtgaagcg gcgcgtgcgg
     7381 gggctcgtcg tcaatgcaag ccgcacggtg gtgcccctga atgggagcgt gagcttcagc
     7441 acgtcgctgg aggccggcag tgatgtgcgc tattcctggg tgctctgtga ccgctgcacg
     7501 cccatccctg ggggtcctac catctcttac accttccgct ccgtgggcac cttcaatatc
     7561 atcgtcacgg ctgagaacga ggtgggctcc gcccaggaca gcatcttcgt ctatgtcctg
     7621 cagctcatag aggggctgca ggtggtgggc ggtggccgct acttccccac caaccacacg
     7681 gtacagctgc aggccgtggt tagggatggc accaacgtct cctacagctg gactgcctgg
     7741 agggacaggg gcccggccct ggccggcagc ggcaaaggct tctcgctcac cgtgctcgag
     7801 gccggcacct accatgtgca gctgcgggcc accaacatgc tgggcagcgc ctgggccgac
     7861 tgcaccatgg acttcgtgga gcctgtgggg tggctgatgg tggccgcctc cccgaaccca
     7921 gctgccgtca acacaagcgt caccctcagt gccgagctgg ctggtggcag tggtgtcgta
     7981 tacacttggt ccttggagga ggggctgagc tgggagacct ccgagccatt taccacccat
     8041 agcttcccca cacccggcct gcacttggtc accatgacgg cagggaaccc gctgggctca
     8101 gccaacgcca ccgtggaagt ggatgtgcag gtgcctgtga gtggcctcag catcagggcc
     8161 agcgagcccg gaggcagctt cgtggcggcc gggtcctctg tgcccttttg ggggcagctg
     8221 gccacgggca ccaatgtgag ctggtgctgg gctgtgcccg gcggcagcag caagcgtggc
     8281 cctcatgtca ccatggtctt cccggatgct ggcaccttct ccatccggct caatgcctcc
     8341 aacgcagtca gctgggtctc agccacgtac aacctcacgg cggaggagcc catcgtgggc
     8401 ctggtgctgt gggccagcag caaggtggtg gcgcccgggc agctggtcca ttttcagatc
     8461 ctgctggctg ccggctcagc tgtcaccttc cgcctgcagg tcggcggggc caaccccgag
     8521 gtgctccccg ggccccgttt ctcccacagc ttcccccgcg tcggagacca cgtggtgagc
     8581 gtgcggggca aaaaccacgt gagctgggcc caggcgcagg tgcgcatcgt ggtgctggag
     8641 gccgtgagtg ggctgcaggt gcccaactgc tgcgagcctg gcatcgccac gggcactgag
     8701 aggaacttca cagcccgcgt gcagcgcggc tctcgggtcg cctacgcctg gtacttctcg
     8761 ctgcagaagg tccagggcga ctcgctggtc atcctgtcgg gccgcgacgt cacctacacg
     8821 cccgtggccg cggggctgtt ggagatccag gtgcgcgcct tcaacgccct gggcagtgag
     8881 aaccgcacgc tggtgctgga ggttcaggac gccgtccagt atgtggccct gcagagcggc
     8941 ccctgcttca ccaaccgctc ggcgcagttt gaggccgcca ccagccccag cccccggcgt
     9001 gtggcctacc actgggactt tggggatggg tcgccagggc aggacacaga tgagcccagg
     9061 gccgagcact cctacctgag gcctggggac taccgcgtgc aggtgaacgc ctccaacctg
     9121 gtgagcttct tcgtggcgca ggccacggtg accgtccagg tgctggcctg ccgggagccg
     9181 gaggtggacg tggtcctgcc cctgcaggtg ctgatgcggc gatcacagcg caactacttg
     9241 gaggcccacg ttgacctgcg cgactgcgtc acctaccaga ctgagtaccg ctgggaggtg
     9301 tatcgcaccg ccagctgcca gcggccgggg cgcccagcgc gtgtggccct gcccggcgtg
     9361 gacgtgagcc ggcctcggct ggtgctgccg cggctggcgc tgcctgtggg gcactactgc
     9421 tttgtgtttg tcgtgtcatt tggggacacg ccactgacac agagcatcca ggccaatgtg
     9481 acggtggccc ccgagcgcct ggtgcccatc attgagggtg gctcataccg cgtgtggtca
     9541 gacacacggg acctggtgct ggatgggagc gagtcctacg accccaacct ggaggacggc
     9601 gaccagacgc cgctcagttt ccactgggcc tgtgtggctt cgacacagag ggaggctggc
     9661 gggtgtgcgc tgaactttgg gccccgcggg agcagcacgg tcaccattcc acgggagcgg
     9721 ctggcggctg gcgtggagta caccttcagc ctgaccgtgt ggaaggccgg ccgcaaggag
     9781 gaggccacca accagacggt gctgatccgg agtggccggg tgcccattgt gtccttggag
     9841 tgtgtgtcct gcaaggcaca ggccgtgtac gaagtgagcc gcagctccta cgtgtacttg
     9901 gagggccgct gcctcaattg cagcagcggc tccaagcgag ggcggtgggc tgcacgtacg
     9961 ttcagcaaca agacgctggt gctggatgag accaccacat ccacgggcag tgcaggcatg
    10021 cgactggtgc tgcggcgggg cgtgctgcgg gacggcgagg gatacacctt cacgctcacg
    10081 gtgctgggcc gctctggcga ggaggagggc tgcgcctcca tccgcctgtc ccccaaccgc
    10141 ccgccgctgg ggggctcttg ccgcctcttc ccactgggcg ctgtgcacgc cctcaccacc
    10201 aaggtgcact tcgaatgcac gggctggcat gacgcggagg atgctggcgc cccgctggtg
    10261 tacgccctgc tgctgcggcg ctgtcgccag ggccactgcg aggagttctg tgtctacaag
    10321 ggcagcctct ccagctacgg agccgtgctg cccccgggtt tcaggccaca cttcgaggtg
    10381 ggcctggccg tggtggtgca ggaccagctg ggagccgctg tggtcgccct caacaggtct
    10441 ttggccatca ccctcccaga gcccaacggc agcgcaacgg ggctcacagt ctggctgcac
    10501 gggctcaccg ctagtgtgct cccagggctg ctgcggcagg ccgatcccca gcacgtcatc
    10561 gagtactcgt tggccctggt caccgtgctg aacgagtacg agcgggccct ggacgtggcg
    10621 gcagagccca agcacgagcg gcagcaccga gcccagatac gcaagaacat cacggagact
    10681 ctggtgtccc tgagggtcca cactgtggat gacatccagc agatcgctgc tgcgctggcc
    10741 cagtgcatgg ggcccagcag ggagctcgta tgccgctcgt gcctgaagca gacgctgcac
    10801 aagctggagg ccatgatgct catcctgcag gcagagacca ccgcgggcac cgtgacgccc
    10861 accgccatcg gagacagcat cctcaacatc acaggagacc tcatccacct ggccagctcg
    10921 gacgtgcggg caccacagcc ctcagagctg ggagccgagt caccatctcg gatggtggcg
    10981 tcccaggcct acaacctgac ctctgccctc atgcgcatcc tcatgcgctc ccgcgtgctc
    11041 aacgaggagc ccctgacgct ggcgggcgag gagatcgtgg cccagggcaa gcgctcggac
    11101 ccgcggagcc tgctgtgcta tggcggcgcc ccagggcctg gctgccactt ctccatcccc
    11161 gaggctttca gcggggccct ggccaacctc agtgacgtgg tgcagctcat ctttctggtg
    11221 gactccaatc cctttccctt tggctatatc agcaactaca ccgtctccac caaggtggcc
    11281 tcgatggcat tccagacaca ggccggcgcc cagatcccca tcgagcggct ggcctcagag
    11341 cgcgccatca ccgtgaaggt gcccaacaac tcggactggg ctgcccgggg ccaccgcagc
    11401 tccgccaact ccgccaactc cgttgtggtc cagccccagg cctccgtcgg tgctgtggtc
    11461 accctggaca gcagcaaccc tgcggccggg ctgcatctgc agctcaacta tacgctgctg
    11521 gacggccact acctgtctga ggaacctgag ccctacctgg cagtctacct acactcggag
    11581 ccccggccca atgagcacaa ctgctcggct agcaggagga tccgcccaga gtcactccag
    11641 ggtgctgacc accggcccta caccttcttc atttccccgg ggagcagaga cccagcgggg
    11701 agttaccatc tgaacctctc cagccacttc cgctggtcgg cgctgcaggt gtccgtgggc
    11761 ctgtacacgt ccctgtgcca gtacttcagc gaggaggaca tggtgtggcg gacagagggg
    11821 ctgctgcccc tggaggagac ctcgccccgc caggccgtct gcctcacccg ccacctcacc
    11881 gccttcggcg ccagcctctt cgtgccccca agccatgtcc gctttgtgtt tcctgagccg
    11941 acagcggatg taaactacat cgtcatgctg acatgtgctg tgtgcctggt gacctacatg
    12001 gtcatggccg ccatcctgca caagctggac cagttggatg ccagccgggg ccgcgccatc
    12061 cctttctgtg ggcagcgggg ccgcttcaag tacgagatcc tcgtcaagac aggctggggc
    12121 cggggctcag gtaccacggc ccacgtgggc atcatgctgt atggggtgga cagccggagc
    12181 ggccaccggc acctggacgg cgacagagcc ttccaccgca acagcctgga catcttccgg
    12241 atcgccaccc cgcacagcct gggtagcgtg tggaagatcc gagtgtggca cgacaacaaa
    12301 gggctcagcc ctgcctggtt cctgcagcac gtcatcgtca gggacctgca gacggcacgc
    12361 agcgccttct tcctggtcaa tgactggctt tcggtggaga cggaggccaa cgggggcctg
    12421 gtggagaagg aggtgctggc cgcgagcgac gcagcccttt tgcgcttccg gcgcctgctg
    12481 gtggctgagc tgcagcgtgg cttctttgac aagcacatct ggctctccat atgggaccgg
    12541 ccgcctcgta gccgtttcac tcgcatccag agggccacct gctgcgttct cctcatctgc
    12601 ctcttcctgg gcgccaacgc cgtgtggtac ggggctgttg gcgactctgc ctacagcacg
    12661 gggcatgtgt ccaggctgag cccgctgagc gtcgacacag tcgctgttgg cctggtgtcc
    12721 agcgtggttg tctatcccgt ctacctggcc atcctttttc tcttccggat gtcccggagc
    12781 aaggtggctg ggagcccgag ccccacacct gccgggcagc aggtgctgga catcgacagc
    12841 tgcctggact cgtccgtgct ggacagctcc ttcctcacgt tctcaggcct ccacgctgag
    12901 gcctttgttg gacagatgaa gagtgacttg tttctggatg attctaagag tctggtgtgc
    12961 tggccctccg gcgagggaac gctcagttgg ccggacctgc tcagtgaccc gtccattgtg
    13021 ggtagcaatc tgcggcagct ggcacggggc caggcgggcc atgggctggg cccagaggag
    13081 gacggcttct ccctggccag cccctactcg cctgccaaat ccttctcagc atcagatgaa
    13141 gacctgatcc agcaggtcct tgccgagggg gtcagcagcc cagcccctac ccaagacacc
    13201 cacatggaaa cggacctgct cagcagcctg tccagcactc ctggggagaa gacagagacg
    13261 ctggcgctgc agaggctggg ggagctgggg ccacccagcc caggcctgaa ctgggaacag
    13321 ccccaggcag cgaggctgtc caggacagga ctggtggagg gtctgcggaa gcgcctgctg
    13381 ccggcctggt gtgcctccct ggcccacggg ctcagcctgc tcctggtggc tgtggctgtg
    13441 gctgtctcag ggtgggtggg tgcgagcttc cccccgggcg tgagtgttgc gtggctcctg
    13501 tccagcagcg ccagcttcct ggcctcattc ctcggctggg agccactgaa ggtcttgctg
    13561 gaagccctgt acttctcact ggtggccaag cggctgcacc cggatgaaga tgacaccctg
    13621 gtagagagcc cggctgtgac gcctgtgagc gcacgtgtgc cccgcgtacg gccaccccac
    13681 ggctttgcac tcttcctggc caaggaagaa gcccgcaagg tcaagaggct acatggcatg
    13741 ctgcggagcc tcctggtgta catgcttttt ctgctggtga ccctgctggc cagctatggg
    13801 gatgcctcat gccatgggca cgcctaccgt ctgcaaagcg ccatcaagca ggagctgcac
    13861 agccgggcct tcctggccat cacgcggtct gaggagctct ggccatggat ggcccacgtg
    13921 ctgctgccct acgtccacgg gaaccagtcc agcccagagc tggggccccc acggctgcgg
    13981 caggtgcggc tgcaggaagc actctaccca gaccctcccg gccccagggt ccacacgtgc
    14041 tcggccgcag gaggcttcag caccagcgat tacgacgttg gctgggagag tcctcacaat
    14101 ggctcgggga cgtgggccta ttcagcgccg gatctgctgg gggcatggtc ctggggctcc
    14161 tgtgccgtgt atgacagcgg gggctacgtg caggagctgg gcctgagcct ggaggagagc
    14221 cgcgaccggc tgcgcttcct gcagctgcac aactggctgg acaacaggag ccgcgctgtg
    14281 ttcctggagc tcacgcgcta cagcccggcc gtggggctgc acgccgccgt cacgctgcgc
    14341 ctcgagttcc cggcggccgg ccgcgccctg gccgccctca gcgtccgccc ctttgcgctg
    14401 cgccgcctca gcgcgggcct ctcgctgcct ctgctcacct cggtgtgcct gctgctgttc
    14461 gccgtgcact tcgccgtggc cgaggcccgt acttggcaca gggaagggcg ctggcgcgtg
    14521 ctgcggctcg gagcctgggc gcggtggctg ctggtggcgc tgacggcggc cacggcactg
    14581 gtacgcctcg cccagctggg tgccgctgac cgccagtgga cccgtttcgt gcgcggccgc
    14641 ccgcgccgct tcactagctt cgaccaggtg gcgcagctga gctccgcagc ccgtggcctg
    14701 gcggcctcgc tgctcttcct gcttttggtc aaggctgccc agcagctacg cttcgtgcgc
    14761 cagtggtccg tctttggcaa gacattatgc cgagctctgc cagagctcct gggggtcacc
    14821 ttgggcctgg tggtgctcgg ggtagcctac gcccagctgg ccatcctgct cgtgtcttcc
    14881 tgtgtggact ccctctggag cgtggcccag gccctgttgg tgctgtgccc tgggactggg
    14941 ctctctaccc tgtgtcctgc cgagtcctgg cacctgtcac ccctgctgtg tgtggggctc
    15001 tgggcactgc ggctgtgggg cgccctacgg ctgggggctg ttattctccg ctggcgctac
    15061 cacgccttgc gtggagagct gtaccggccg gcctgggagc cccaggacta cgagatggtg
    15121 gagttgttcc tgcgcaggct gcgcctctgg atgggcctca gcaaggtcaa ggagttccgc
    15181 cacaaagtcc gctttgaagg gatggagccg ctgccctctc gctcctccag gggctccaag
    15241 gtatccccgg atgtgccccc acccagcgct ggctccgatg cctcgcaccc ctccacctcc
    15301 tccagccagc tggatgggct gagcgtgagc ctgggccggc tggggacaag gtgtgagcct
    15361 gagccctccc gcctccaagc cgtgttcgag gccctgctca cccagtttga ccgactcaac
    15421 caggccacag aggacgtcta ccagctggag cagcagctgc acagcctgca aggccgcagg
    15481 agcagccggg cgcccgccgg atcttcccgt ggcccatccc cgggcctgcg gccagcactg
    15541 cccagccgcc ttgcccgggc cagtcggggt gtggacctgg ccactggccc cagcaggaca
    15601 ccccttcggg ccaagaacaa ggtccacccc agcagcactg actacaagga cgacgatgac
    15661 aagtgatcag gtagcttatc gataccgtcg acccgggcgg ccgccgcttc gagcagacat
    15721 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
    15781 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
    15841 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt
    15901 tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc aattgccttt
    15961 ttttttaatt tttattttat tttatttttg agatggccag atccgggctg gcgtaatagc
    16021 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg
    16081 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
    16141 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
    16201 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
    16261 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
    16321 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
    16381 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
    16441 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
    16501 cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca
    16561 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
    16621 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
    16681 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
    16741 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
    16801 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
    16861 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
    16921 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
    16981 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
    17041 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
    17101 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
    17161 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
    17221 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
    17281 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
    17341 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
    17401 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
    17461 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
    17521 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
    17581 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
    17641 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
    17701 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
    17761 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
    17821 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaac
//
