LOCUS       sequence-31294-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBA05 (3H8V)
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..97
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccctctgaat attttgttta ctttaagaag gagatatacc atgggcagca gccatcatca
       61 tcatcatcac agcagcggcc tggttccgcg tggtagtatg gcattgaaac gaatgggaat
      121 tgtaagcgac tatgagaaaa tccgtacctt tgccgtagca atagtaggtg ttggtggagt
      181 aggtagtgtg actgctgaaa tgctgacaag atgtggcatt ggtaagttgc tactctttga
      241 ttatgacaag gtggaactag ccaatatgaa tagacttttc ttccaacctc atcaagcagg
      301 attaagtaaa gttcaagcag cagaacatac tctgaggaac attaatcctg atgttctttt
      361 tgaagtacac aactataata taaccacagt ggaaaacttt caacatttca tggatagaat
      421 aagtaatggt gggttagaag aaggaaaacc tgttgatcta gttcttagct gtgtggacaa
      481 ttttgaagct cgaatgacaa taaatacagc ttgtaatgaa cttggacaaa catggatgga
      541 atctggggtc agtgaaaatg cagtttcagg gcatatacag cttataattc ctggagaatc
      601 tgcttgtttt gcgtgtgctc caccacttgt agttgctgca aatattgatg aaaagactct
      661 gaaacgagaa ggtgtttgtg cagccagtct tcctaccact atgggtgtgg ttgctgggat
      721 cttagtacaa aacgtgttaa agtttctgtt aaattttggt actgttagtt tttaccttgg
      781 atacaatgca atgcaggatt tttttcctac tatgtccatg aagccaaatc ctcagtgtga
      841 tgacagaaat tgcaggaagc agcaggagga atataagaaa aaggtagcag cactgcct
//
