LOCUS       sequence-31295-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GABARAPL1
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..95
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             452..469
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(522..540)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      536..583
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(707..726)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttcctctgaa tattttgttt actttaagaa ggagatatac catgcatcat catcatcatc
       61 acagcagcgg cagagaaaac ttgtatttcc agggcttcca gtacaaggag gaccatccct
      121 ttgagtatcg gaaaaaggaa ggagaaaaga tccggaagaa atatccggac agggtccccg
      181 tgattgtaga gaaggctcca aaagccaggg tgcctgatct ggacaagagg aagtacctag
      241 tgccctctga ccttactgtt ggccagttct acttcttaat ccggaagaga atccacctga
      301 gacctgagga cgccttattc ttctttgtca acaacaccat ccctcccacc agtgctacca
      361 tgggccaact gtatgaggac aatcatgagg aagactattt tctgtatgtg gcctacagtg
      421 attgatgacg aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc
      481 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      541 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      601 atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      661 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      721 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
      781 cctttagggt tccgatttag tgctttacgg cacctcga
//
