LOCUS       sequence-312953-        3590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 Golden Gate vector; Unmodifed CRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Harrington SA, Backhaus AE, Fox S, Rogers C, Borrill P, Uauy C, 
            Richardson A
  TITLE     A heat-shock inducible system for flexible gene expression in 
            cereals.
  JOURNAL   Plant Methods. 2020 Oct 14;16:137. doi: 10.1186/s13007-020-00677-3. 
            eCollection 2020.
  PUBMED    33072173
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2020-10-14"; volume: "16:137. doi"; pages: " 
            10.1186/s13007-020-00677-3. eCollection 2020"
COMMENT     Sequence Label: EC71102
FEATURES             Location/Qualifiers
     source          1..3590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     102..124
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     116..133
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     117..133
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     144..163
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        144..162
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             224..1255
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     primer_bind     complement(1404..1421)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1514..2102)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1594..1613)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(3287..3391)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgttgggaag ggcgtttcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
       61 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
      121 aacgacggcc agtgagcgcg acgtaatacg actcactata gggcgaattg agtgaaggcc
      181 gtcaaggcct aggcgcgcca tgagctccac tctgtggtct caaatgtcca atttactgac
      241 cgtacaccaa aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa
      301 cctgatggac atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct
      361 gtccgtttgc cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc
      421 agaacctgaa gatgttcgcg attatcttct atatcttcag gcgcgcggtc tggcagtaaa
      481 aactatccag caacatttgg gccagctaaa catgcttcat cgtcggtccg ggctgccacg
      541 accaagtgac agcaatgctg tttcactggt tatgcggcgg atccgaaaag aaaacgttga
      601 tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc actgatttcg accaggttcg
      661 ttcactcatg gaaaatagcg atcgctgcca ggatatacgt aatctggcat ttctggggat
      721 tgcttataac accctgttac gtatagccga aattgccagg atcagggtta aagatatctc
      781 acgtactgac ggtgggagaa tgttaatcca tattggcaga acgaaaacgc tggttagcac
      841 cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc gatggatttc
      901 cgtttctggt gtagctgatg atccgaataa ctacctgttt tgccgggtca gaaaaaatgg
      961 tgttgccgcg ccatctgcca ccagccagct atcaactcgc gccctggaag ggatttttga
     1021 agcaactcat cgattgattt acggcgctaa ggatgactct ggtcagagat acctggcctg
     1081 gtctggacac agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg gagtttcaat
     1141 accggagatc atgcaagctg gtggctggac caatgtaaat attgtcatga actatatccg
     1201 taacctggat agtgaaacag gggcaatggt gcgcctgctg gaagatggcg attaggcttt
     1261 gagaccacga agtgggtacc tcttaattaa ctggcctcat gggccttcac ttcactgccc
     1321 gctttccagt cgggaaacct gtcgtgccag ctgcattaac atggtcatag ctgtttcctt
     1381 gcgtattggg cgctctccgc ttcctcgctc actgactcgc tgcgctcggt cgttcgggta
     1441 aagcctgggg tgcctaatga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1501 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1561 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1621 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1681 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1741 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1801 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1861 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1921 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1981 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2041 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2101 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2161 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2221 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttattatt
     2281 tacccaccac tttggtaatt tcgcctttca cgtaatgcac aaattcttcc agctgatccg
     2341 cacggctggc cagacgatct tcttcctggc ccagatacgc ctgacgcgct tccagaatca
     2401 ccggctgata ctgcgccggc agacgttcca tggcccaatc cgccgccaca tctttcggcg
     2461 caattttgcc ggtcaccgcg ctataccaaa tacggctcag ggtcagcacc acgttgcgtt
     2521 catcacccgc ccaatccggc gggctgttcc acagggtcag ggtttcgttc agcgcttcaa
     2581 acaggtcctg ttccggcacc ggatcaaaca gttcttccgc tgccggacca accagcgcca
     2641 cgctatgttc acgcgctttg gtcagcagaa tggccagatc gatatcaatg gtcgccggtt
     2701 caaaaatgcc ggccagaata tcgttacgct gccattcgcc aaactgcagc tcacgtttcg
     2761 ccggataacg ccacggaata atgtcatcat gcaccacgat ggtcacttcc accgcacgca
     2821 gaatttcgct ttcacccggg ctcgcgctgg tttccagcag atcgttaatc agcgcacgac
     2881 gggtggtttc atccagacgc acggtcacgg tcaccagcag atcaatatcg ctatgcggtt
     2941 tcaggccgcc atccaccgcg ctgccataca gatgcacggc cagcagggtc ggttccagat
     3001 gacgttcaat cacgcccacc acttcagaca gctgggtgct cacttccgca atcaccgctt
     3061 cacgcataat gttcagtttg gttttcggac ggctgccctg ctgggtcaca tcgttgctgc
     3121 tccacagcat caggcaacgg cccaccgcat aacgcgcctg ctgtttgctc gcacgcggca
     3181 tgctctgcac gccaaaaaag caatcatagc acgccataaa caccgccacc gcaccattac
     3241 cgccgctacg ttcggtcagg gtacggctcc aattacggct acgcatactc ttcctttttc
     3301 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     3361 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     3421 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     3481 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     3541 gttgagtggc cgctacaggg cgctcccatt cgccattcag gctgcgcaac
//
