LOCUS       sequence-31296-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MALT1 (3BFO)
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             398..415
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(468..486)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      482..529
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(653..672)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aattccctct gaatattttg tttactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggttc cgcgtggtag taagttgcaa atctgtgttg
      121 aaccaacttc ccaaaagctg atgccaggca gcacattggt tttacagtgt gttgctgttg
      181 gaagccctat tcctcactac cagtggttca aaaatgaatt accattaaca catgagacca
      241 aaaagctata catggtgcct tatgtggatt tggaacacca aggaacctac tggtgtcatg
      301 tatataatga tcgagacagt caagatagca agaaggtaga aatcatcata gatgaattaa
      361 ataatctttg atgacgaagc ttgcggccgc actcgagcac caccaccacc accactgaga
      421 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      481 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
      541 aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
      601 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
      661 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
      721 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
      781 tagggtgatg gttcacgtag tgggccatcg ccctgataga cg
//
