LOCUS       sequence-312974-       11012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi flaB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11012)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 11012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S_PresTL-sgRNAflaB
FEATURES             Location/Qualifiers
     source          1..11012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        150..225
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(1668..1682,1683..1688,1689..1705,1706..1712)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    1688..1704
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1720..1736
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1755..1766
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(1773..1799,1800..1802,1803..4289,4290..4292,
                     4293..5879)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6049..6840
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6963..7551
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7452..7471
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaatatttct ttggtttatt atgcatccta gtacatatta tataatttaa tttaatatat
       61 aagcatattt ttttaaaatc agaaattttt tacttagcta aagattttaa acttggtata
      121 attgaattga aaatttaaat ttctgacttg ttttagagct agaaatagca agttaaaata
      181 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttggcgcgcc
      241 aaataatgcc cgtgtccctg ttgaataggg acacggtttt ttaacaattt ttttattcct
      301 aggccttagt ggccggtacc cggggatcct tattactggc cgctttctag cctggatact
      361 tgtctggcta gttgcattag agagtcagct agagccctag ggctagctgt ttgtgtattc
      421 ggagccagtg tagtttttct ttttactaac gatacgggta ataattgatt gccttttaca
      481 gcctgacctt gagacaattg caaaagtctg tccacagagg tttgacctaa taatctaaaa
      541 tcttgtttga tagtagtaag tggaggtatg tagcaactag aatcttctgt atcatcataa
      601 cccacaactg aaatatcagc cccaactcta agtccacttt cagtaatagc tcgcatagct
      661 cctaaagcca tttgatcatt agcaactaac atagcagtag ggacaatgcc ttcatttaac
      721 atttgcattg tttgctgaaa tcctgacata gcagaccaat ctccttctct ttctgctatt
      781 ggttgtattt gatttcttgt taaatacttg tgccatcctg ctaatctcaa tctggcactt
      841 actgaactta agggacctgc aagtagtgca atttgttgat ggcctaatgc aacaaggtgt
      901 tcaactccta accttgttcc atcttcgtga gaaaaaatta tagagtttat tggggtttgg
      961 tccgacacat ccaaaaacaa tgctggaaca ttagtacatg ctgcttcaac tgcaatcgca
     1021 tcttggtcat caagaggata attaattata agtccactaa cacgttgagc taataaatta
     1081 tgtactgctg ccttacaagc ctctacacct gatctctcaa ccattgatac aacaacgctt
     1141 gcaccaagtt gatcagctct cgatttaatt gcggctacaa tttgactagg cgcatgtaat
     1201 gcaagtgatg atgtggcaac tccaattaaa agtgattgtt tccccgcaag ttgctgagca
     1261 acgcgatttg gtatataatt caactctgcc atagctgctt caaccttctc tcttgttttt
     1321 gcagatacat gacttgcttg gttcacaact ctcgaaaccg tttggtagct aaccccggca
     1381 tattcagcaa catcatacaa agtaacaggt ttcatggatc attcctccat gataaaattt
     1441 aaatttctga ctttggcaaa tccttttgcc tgttaaaaag aatagcacaa aaattaaaaa
     1501 aaaagaaacg caaatagcaa tagcattaaa aataattatt attatttatt tctttgatta
     1561 aaatcaaaag cctttaatgt attttcttga tctttcatgc gcttaacggt aaaatccaag
     1621 gtattaagtt caccttccac ttttctctct ctatcttctc tagaaaatca taaaaaattt
     1681 atttgctttg tgagcggata acaattataa tagattcaat tgtgagcgga taacaatttc
     1741 acacagaatt cattaaagag gagaaattac atatggataa gaaatactca ataggcttag
     1801 ctatcggcac aaatagcgtc ggatgggcgg tgatcactga tgaatataag gttccgtcta
     1861 aaaagttcaa ggttctggga aatacagacc gccacagtat caaaaaaaat cttatagggg
     1921 ctcttttatt tgacagtgga gagacagcgg aagcgactcg tctcaaacgg acagctcgta
     1981 gaaggtatac acgtcggaag aatcgtattt gttatctaca ggagattttt tcaaatgaga
     2041 tggcgaaagt agatgatagt ttctttcatc gacttgaaga gtcttttttg gtggaagaag
     2101 acaagaagca tgaacgtcat cctatttttg gaaatatagt agatgaagtt gcttatcatg
     2161 agaaatatcc aactatctat catctgcgaa aaaaattggt agattctact gataaagcgg
     2221 atttgcgctt aatctatttg gccttagcgc atatgattaa gtttcgtggt cattttttga
     2281 ttgagggaga tttaaatcct gataatagtg atgtggacaa actatttatc cagttggtac
     2341 aaacctacaa tcaattattt gaagaaaacc ctattaacgc aagtggagta gatgctaaag
     2401 cgattctttc tgcacgattg agtaaatcaa gacgattaga aaatctcatt gctcagctcc
     2461 ccggtgagaa gaaaaatggc ttatttggga atctcattgc tttgtcattg ggtttgaccc
     2521 ctaattttaa atcaaatttt gatttggcag aagatgctaa attacagctt tcaaaagata
     2581 cttacgatga tgatttagat aatttattgg cgcaaattgg agatcaatat gctgatttgt
     2641 ttttggcagc taagaattta tcagatgcta ttttactttc agatatccta agagtaaata
     2701 ctgaaataac taaggctccc ctatcagctt caatgattaa acgctacgat gaacatcatc
     2761 aagacttgac tcttttaaaa gctttagttc gacaacaact tccagaaaag tataaagaaa
     2821 tcttttttga tcaatcaaaa aacggatatg caggttatat tgatggggga gctagccaag
     2881 aagaatttta taaatttatc aaaccaattt tagaaaaaat ggatggtact gaggaattat
     2941 tggtgaaact aaatcgtgaa gatttgctgc gcaagcaacg gacctttgac aacggctcta
     3001 ttccccatca aattcacttg ggtgagctgc atgctatttt gagaagacaa gaagactttt
     3061 atccattttt aaaagacaat cgtgagaaga ttgaaaaaat cttgactttt cgaattcctt
     3121 attatgttgg tccattggcg cgtggcaata gtcgttttgc atggatgact cggaagtctg
     3181 aagaaacaat taccccatgg aattttgaag aagttgtcga taaaggtgct tcagctcaat
     3241 catttattga acgcatgaca aactttgata aaaatcttcc aaatgaaaaa gtactaccaa
     3301 aacatagttt gctttatgag tattttacgg tttataacga attgacaaag gtcaaatatg
     3361 ttactgaagg aatgcgaaaa ccagcatttc tttcaggtga acagaagaaa gccattgttg
     3421 atttactctt caaaacaaat cgaaaagtaa ccgttaagca attaaaagaa gattatttca
     3481 aaaaaataga atgttttgat agtgttgaaa tttcaggagt tgaagataga tttaatgctt
     3541 cattaggtac ctaccatgat ttgctaaaaa ttattaaaga taaagatttt ttggataatg
     3601 aagaaaatga agatatctta gaggatattg ttttaacatt gaccttattt gaagataggg
     3661 agatgattga ggaaagactt aaaacatatg ctcacctctt tgatgataag gtgatgaaac
     3721 agcttaaacg tcgccgttat actggttggg gacgtttgtc tcgaaaattg attaatggta
     3781 ttagggataa gcaatctggc aaaacaatat tagatttttt gaaatcagat ggttttgcca
     3841 atcgcaattt tatgcagctg atccatgatg atagtttgac atttaaagaa gacattcaaa
     3901 aagcacaagt gtctggacaa ggcgatagtt tacatgaaca tattgcaaat ttagctggta
     3961 gccctgctat taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag
     4021 taatggggcg gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa
     4081 ctcaaaaggg ccagaaaaat tcgcgagagc gtatgaaacg aatcgaagaa ggtatcaaag
     4141 aattaggaag tcagattctt aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa
     4201 agctctatct ctattatctc caaaatggaa gagacatgta tgtggaccaa gaattagata
     4261 ttaatcgttt aagtgattat gatgtcgatg ccattgttcc acaaagtttc cttaaagacg
     4321 attcaataga caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa tcggataacg
     4381 ttccaagtga agaagtagtc aaaaagatga aaaactattg gagacaactt ctaaacgcca
     4441 agttaatcac tcaacgtaag tttgataatt taacgaaagc tgaacgtgga ggtttgagtg
     4501 aacttgataa agctggtttt atcaaacgcc aattggttga aactcgccaa atcactaagc
     4561 atgtggcaca aattttggat agtcgcatga atactaaata cgatgaaaat gataaactta
     4621 ttcgagaggt taaagtgatt accttaaaat ctaaattagt ttctgacttc cgaaaagatt
     4681 tccaattcta taaagtacgt gagattaaca attaccatca tgcccatgat gcgtatctaa
     4741 atgccgtcgt tggaactgct ttgattaaga aatatccaaa acttgaatcg gagtttgtct
     4801 atggtgatta taaagtttat gatgttcgta aaatgattgc taagtctgag caagaaatag
     4861 gcaaagcaac cgcaaaatat ttcttttact ctaatatcat gaacttcttc aaaacagaaa
     4921 ttacacttgc aaatggagag attcgcaaac gccctctaat cgaaactaat ggggaaactg
     4981 gagaaattgt ctgggataaa gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc
     5041 cccaagtcaa tattgtcaag aaaacagaag tccagacagg cggattctcc aaggagtcaa
     5101 ttttaccaaa aagaaattcg gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa
     5161 aatatggtgg ttttgatagt ccaacggtag cttattcagt cctagtggtt gctaaggtgg
     5221 aaaaagggaa atcgaagaag ttaaaatccg ttaaagagtt actagggatc acaattctgg
     5281 aaagaagttc ctttgaaaaa aatccgattg actttttaga agctaaagga tataaggaag
     5341 ttaaaaaaga cttaatcatt aaactaccta aatatagtct ttttgagtta gaaaacggtc
     5401 gtaaacggat gctggctagt gccggagaat tacaaaaagg aaatgagctg gctctgccaa
     5461 gcaaatatgt gaatttttta tatttagcta gtcattatga aaagttgaag ggtagtccag
     5521 aagataacga acaaaaacaa ttgtttgtgg agcagcataa gcattattta gatgagatta
     5581 ttgagcaaat cagtgaattt tctaagcgtg ttattttagc agatgccaat ttagataaag
     5641 ttcttagtgc atataacaaa catagagaca aaccaatacg tgaacaagca gaaaatatta
     5701 ttcatttatt tacgttgacg aatcttggag ctcccgctgc ttttaaatat tttgatacaa
     5761 caattgatcg taaacgatat acgtctacaa aagaagtttt agatgccact cttatccatc
     5821 aatccatcac tggtctttat gaaacacgca ttgatttgag tcagctagga ggtgactaat
     5881 aaaagcttga tcagatctca gctttttttt gaagtgcctg gcagtaagtt gaaaaaattt
     5941 gaaataaact tggactatgt taataaaaaa atacaaatta acgaacaaaa agatttgaat
     6001 tattatataa tttagttaaa agcaatttta aatgagggag gtttccatat gagggaagcg
     6061 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     6121 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     6181 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     6241 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     6301 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     6361 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     6421 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     6481 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     6541 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     6601 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     6661 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     6721 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     6781 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
     6841 tgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctcat gaccaaaatc
     6901 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6961 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7021 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7081 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     7141 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7201 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7261 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7321 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7381 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7441 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7501 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7561 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7621 gcactagatc cccggcaagt tcatccttag ggttttcata tctcttaaaa agtcaatttc
     7681 tgcttgatta atttcttgtg gattattgtt tttgcggttt ttcatttttt atctccttga
     7741 gtattgaatt ttttcttata tataaatata tagcaaaaac tatttttgcc aactttttta
     7801 caaaaaattt tttattaaaa cacttagggc tttgctaaat tctcttttaa aagaacttag
     7861 taaagcccta tggatttaag aactgattat acttacgtag taaaaatact acagatatta
     7921 atagtataga ccatatcaaa atcataatca atttatattt atctaaaatt gtagtccata
     7981 caaaatacaa aaaaattagg tttttctaaa ttctatttta aaagaactta gtaacaccta
     8041 atctctaaat tacttacaaa attctaaaat tagattttta gttcttcata tttctttaaa
     8101 agttttttaa gaaaatcttt ttgattttca aaaatttcat gcatcataaa actagtaaat
     8161 ttggattttt ttttgtaaaa atcataactt tcttgggttt taagttgaaa tcttaatggt
     8221 tttattggat tttgcctaga tttttttaac gccggacttt ctttatcttt tataaaatta
     8281 taagaatttt taataccatt ttctattaaa taatcttctt ttataagccc ctcttgcagt
     8341 gcagttgcta atcttaaata cttatacgct tgagtttttg ctacatcaaa gtcttttata
     8401 aaatcaagaa aagttttata accatcaaat ttataatatt gattatcttt tatttctttt
     8461 aaaattttaa tattttccac cctgtggaat atttcttttt tgaaattgtc atttaacttt
     8521 tcctttagtg tcttgtaata agaaatttta ctttcattaa tcattaaatt ttgttcaaca
     8581 taagtatcta tcttttttgt taaaacttta acaacacgtt ttttttccat aaaaattttc
     8641 ctttattttt gcaacatttt ccttaattga tataattgtt actattttag attttttaat
     8701 atccacttaa gaattaatcc ctcactagaa tattttttat tatagaattc atatacatgt
     8761 ttttctaaat ctataaattt atcaacaatt gctttattat agggtgtatc tatcttttct
     8821 tttcttaata atcgtctcat attctttaaa taacaacaaa cactgccggt tttaaaccta
     8881 aattctatgt aatatatttt tgaaaaagta tatacatttt caaccccgtt tatttcatac
     8941 tttacaaaaa cattatttat tggttttcta taaccatagt aaatgcctaa aaatttgtcc
     9001 ccgtctttca taggatatag ataaagtttt tgaatctctg atttatctaa taatttttca
     9061 aaaaaaatta tacaccgctt ttgtttgtta ctaactactc tatgcaatgc ctttaatatt
     9121 tttgtgtgat atatattttt atcacctttt ttttctttca gcaggaagat actttctttt
     9181 ggaaatttaa ttttttgttc atttattctt gcagccttaa gtccgatttt catatttaat
     9241 ccttatatag cctttctatt taattctttt aaactcaata aacttttttg gtttttcatt
     9301 agttctaata attcgcaata atatgtgcaa aacactttat tgtattctat tttcttttgg
     9361 ttattcaaat attcttttat aattggcttt agaagttcaa tatttacttc tttttttagt
     9421 tgttcaataa ggatattgaa aatgtttgtt tttaaatctt gataattttg ttgagaattt
     9481 tctttttttc tttcaattga cttctctaat ttacgcttta tattgtttaa atcgctatat
     9541 ttatgacttt caataataaa atgaggctta aatttgtaat tttcgtatat tttttggaaa
     9601 cttgtttcta attgtttaga attatattca tttttttcaa attctttttg agtgttgcat
     9661 agaatttcct ttaatttttc ttgtttttct ttcatacaag atttagtaaa atgtatattt
     9721 ttatttttta gtaatttaat ttctattatt tttatgattt taagtatttt gattatatta
     9781 tctttatcaa tatttaaatt gaataaaatt gtaagaaaaa ttgaaagaat ttttttacat
     9841 aaaaagttac atttattgaa gtaatttctt acttgatact tttctatttc attaatcttt
     9901 ctttcttctt ttatattatt tttattattt aaacactcct ttaaatctac actcccattt
     9961 ttagtaaatc tatctttaag gccccttgaa actctatttt gaaatcttaa atcttttttt
    10021 tcttgaaagt atttatagat tttttggtaa cattcttttt ttgaaaaatt aagttgatag
    10081 tatatttcgg tccccaaatt tacccctaag tgtttgtgat aattagttgt gactttaaat
    10141 tctttttcta atttgtaaag gtaattttgc agtgttttta gtttaacggg tgtttgacta
    10201 tttcttttaa ggttttcatt aaagtaatag agtatgtttt tttgtgtgta tttcttatat
    10261 gttttattta tgtacgctag tgttgagata aggactacta atttatactg taatttattg
    10321 tagcaagttg tggttttact ggttttgttt atattagata agttttccat ttatagctcc
    10381 ttgaataccg gattttttat ctataaagta tatatatcac aattttgatt taaaagtaaa
    10441 ttcttttttt aaaaatacaa aaatttttta taaattatat agaccatgat attgtattaa
    10501 tactaattga ttttaattta aggttgaact aaaataaaca tagttttgta gaaaataatt
    10561 tttcattatt ttgctacttg atattggatc gtaaaaatat tagggcttta caaagttctt
    10621 ttaaaagaga atttagtaaa gccctaagtc ttttaataaa aaattttttg taaaaaagtt
    10681 ggcaaaaata gtttttgcta tatatttata tataagaaaa aattcaatac tcaaggagat
    10741 aaaaaatgga aaacttaaac aataatccac aaaaaattaa tcaaataaat aagcaaacta
    10801 attttattgg ttttgaaaat tttatagaac ttgataatca agttaatcaa actaaagaaa
    10861 aaagtaaatt aagtaagata ggtaagaaat tacccggaat aagcagtcaa gtctagtgcg
    10921 gccggaaaca gctatgacat gattacgaat tcgagctccg aagtttatta ttttatgatt
    10981 taaattaata aaaagattta tcggtgtgaa ta
//
