LOCUS       sequence-312975-       10937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi flaB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10937)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S_PresTS-sgRNAflaB
FEATURES             Location/Qualifiers
     source          1..10937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1443..1457,1458..1463,1464..1480,1481..1487)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    1463..1479
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1495..1511
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1530..1541
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(1548..1574,1575..1577,1578..4064,4065..4067,
                     4068..5654)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5824..6615
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6738..7326
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7227..7246
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_RNA        10862..10937
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tttttttggc gcgccaaata atgcccgtgt ccctgttgaa tagggacacg gttttttaac
       61 aattttttta ttcctaggcc ttagtggccg gtacccgggg atccttatta ctggccgctt
      121 tctagcctgg atacttgtct ggctagttgc attagagagt cagctagagc cctagggcta
      181 gctgtttgtg tattcggagc cagtgtagtt tttcttttta ctaacgatac gggtaataat
      241 tgattgcctt ttacagcctg accttgagac aattgcaaaa gtctgtccac agaggtttga
      301 cctaataatc taaaatcttg tttgatagta gtaagtggag gtatgtagca actagaatct
      361 tctgtatcat cataacccac aactgaaata tcagccccaa ctctaagtcc actttcagta
      421 atagctcgca tagctcctaa agccatttga tcattagcaa ctaacatagc agtagggaca
      481 atgccttcat ttaacatttg cattgtttgc tgaaatcctg acatagcaga ccaatctcct
      541 tctctttctg ctattggttg tatttgattt cttgttaaat acttgtgcca tcctgctaat
      601 ctcaatctgg cacttactga acttaaggga cctgcaagta gtgcaatttg ttgatggcct
      661 aatgcaacaa ggtgttcaac tcctaacctt gttccatctt cgtgagaaaa aattatagag
      721 tttattgggg tttggtccga cacatccaaa aacaatgctg gaacattagt acatgctgct
      781 tcaactgcaa tcgcatcttg gtcatcaaga ggataattaa ttataagtcc actaacacgt
      841 tgagctaata aattatgtac tgctgcctta caagcctcta cacctgatct ctcaaccatt
      901 gatacaacaa cgcttgcacc aagttgatca gctctcgatt taattgcggc tacaatttga
      961 ctaggcgcat gtaatgcaag tgatgatgtg gcaactccaa ttaaaagtga ttgtttcccc
     1021 gcaagttgct gagcaacgcg atttggtata taattcaact ctgccatagc tgcttcaacc
     1081 ttctctcttg tttttgcaga tacatgactt gcttggttca caactctcga aaccgtttgg
     1141 tagctaaccc cggcatattc agcaacatca tacaaagtaa caggtttcat ggatcattcc
     1201 tccatgataa aatttaaatt tctgactttg gcaaatcctt ttgcctgtta aaaagaatag
     1261 cacaaaaatt aaaaaaaaag aaacgcaaat agcaatagca ttaaaaataa ttattattat
     1321 ttatttcttt gattaaaatc aaaagccttt aatgtatttt cttgatcttt catgcgctta
     1381 acggtaaaat ccaaggtatt aagttcacct tccacttttc tctctctatc ttctctagaa
     1441 aatcataaaa aatttatttg ctttgtgagc ggataacaat tataatagat tcaattgtga
     1501 gcggataaca atttcacaca gaattcatta aagaggagaa attacatatg gataagaaat
     1561 actcaatagg cttagctatc ggcacaaata gcgtcggatg ggcggtgatc actgatgaat
     1621 ataaggttcc gtctaaaaag ttcaaggttc tgggaaatac agaccgccac agtatcaaaa
     1681 aaaatcttat aggggctctt ttatttgaca gtggagagac agcggaagcg actcgtctca
     1741 aacggacagc tcgtagaagg tatacacgtc ggaagaatcg tatttgttat ctacaggaga
     1801 ttttttcaaa tgagatggcg aaagtagatg atagtttctt tcatcgactt gaagagtctt
     1861 ttttggtgga agaagacaag aagcatgaac gtcatcctat ttttggaaat atagtagatg
     1921 aagttgctta tcatgagaaa tatccaacta tctatcatct gcgaaaaaaa ttggtagatt
     1981 ctactgataa agcggatttg cgcttaatct atttggcctt agcgcatatg attaagtttc
     2041 gtggtcattt tttgattgag ggagatttaa atcctgataa tagtgatgtg gacaaactat
     2101 ttatccagtt ggtacaaacc tacaatcaat tatttgaaga aaaccctatt aacgcaagtg
     2161 gagtagatgc taaagcgatt ctttctgcac gattgagtaa atcaagacga ttagaaaatc
     2221 tcattgctca gctccccggt gagaagaaaa atggcttatt tgggaatctc attgctttgt
     2281 cattgggttt gacccctaat tttaaatcaa attttgattt ggcagaagat gctaaattac
     2341 agctttcaaa agatacttac gatgatgatt tagataattt attggcgcaa attggagatc
     2401 aatatgctga tttgtttttg gcagctaaga atttatcaga tgctatttta ctttcagata
     2461 tcctaagagt aaatactgaa ataactaagg ctcccctatc agcttcaatg attaaacgct
     2521 acgatgaaca tcatcaagac ttgactcttt taaaagcttt agttcgacaa caacttccag
     2581 aaaagtataa agaaatcttt tttgatcaat caaaaaacgg atatgcaggt tatattgatg
     2641 ggggagctag ccaagaagaa ttttataaat ttatcaaacc aattttagaa aaaatggatg
     2701 gtactgagga attattggtg aaactaaatc gtgaagattt gctgcgcaag caacggacct
     2761 ttgacaacgg ctctattccc catcaaattc acttgggtga gctgcatgct attttgagaa
     2821 gacaagaaga cttttatcca tttttaaaag acaatcgtga gaagattgaa aaaatcttga
     2881 cttttcgaat tccttattat gttggtccat tggcgcgtgg caatagtcgt tttgcatgga
     2941 tgactcggaa gtctgaagaa acaattaccc catggaattt tgaagaagtt gtcgataaag
     3001 gtgcttcagc tcaatcattt attgaacgca tgacaaactt tgataaaaat cttccaaatg
     3061 aaaaagtact accaaaacat agtttgcttt atgagtattt tacggtttat aacgaattga
     3121 caaaggtcaa atatgttact gaaggaatgc gaaaaccagc atttctttca ggtgaacaga
     3181 agaaagccat tgttgattta ctcttcaaaa caaatcgaaa agtaaccgtt aagcaattaa
     3241 aagaagatta tttcaaaaaa atagaatgtt ttgatagtgt tgaaatttca ggagttgaag
     3301 atagatttaa tgcttcatta ggtacctacc atgatttgct aaaaattatt aaagataaag
     3361 attttttgga taatgaagaa aatgaagata tcttagagga tattgtttta acattgacct
     3421 tatttgaaga tagggagatg attgaggaaa gacttaaaac atatgctcac ctctttgatg
     3481 ataaggtgat gaaacagctt aaacgtcgcc gttatactgg ttggggacgt ttgtctcgaa
     3541 aattgattaa tggtattagg gataagcaat ctggcaaaac aatattagat tttttgaaat
     3601 cagatggttt tgccaatcgc aattttatgc agctgatcca tgatgatagt ttgacattta
     3661 aagaagacat tcaaaaagca caagtgtctg gacaaggcga tagtttacat gaacatattg
     3721 caaatttagc tggtagccct gctattaaaa aaggtatttt acagactgta aaagttgttg
     3781 atgaattggt caaagtaatg gggcggcata agccagaaaa tatcgttatt gaaatggcac
     3841 gtgaaaatca gacaactcaa aagggccaga aaaattcgcg agagcgtatg aaacgaatcg
     3901 aagaaggtat caaagaatta ggaagtcaga ttcttaaaga gcatcctgtt gaaaatactc
     3961 aattgcaaaa tgaaaagctc tatctctatt atctccaaaa tggaagagac atgtatgtgg
     4021 accaagaatt agatattaat cgtttaagtg attatgatgt cgatgccatt gttccacaaa
     4081 gtttccttaa agacgattca atagacaata aggtcttaac gcgttctgat aaaaatcgtg
     4141 gtaaatcgga taacgttcca agtgaagaag tagtcaaaaa gatgaaaaac tattggagac
     4201 aacttctaaa cgccaagtta atcactcaac gtaagtttga taatttaacg aaagctgaac
     4261 gtggaggttt gagtgaactt gataaagctg gttttatcaa acgccaattg gttgaaactc
     4321 gccaaatcac taagcatgtg gcacaaattt tggatagtcg catgaatact aaatacgatg
     4381 aaaatgataa acttattcga gaggttaaag tgattacctt aaaatctaaa ttagtttctg
     4441 acttccgaaa agatttccaa ttctataaag tacgtgagat taacaattac catcatgccc
     4501 atgatgcgta tctaaatgcc gtcgttggaa ctgctttgat taagaaatat ccaaaacttg
     4561 aatcggagtt tgtctatggt gattataaag tttatgatgt tcgtaaaatg attgctaagt
     4621 ctgagcaaga aataggcaaa gcaaccgcaa aatatttctt ttactctaat atcatgaact
     4681 tcttcaaaac agaaattaca cttgcaaatg gagagattcg caaacgccct ctaatcgaaa
     4741 ctaatgggga aactggagaa attgtctggg ataaagggcg agattttgcc acagtgcgca
     4801 aagtattgtc catgccccaa gtcaatattg tcaagaaaac agaagtccag acaggcggat
     4861 tctccaagga gtcaatttta ccaaaaagaa attcggacaa gcttattgct cgtaaaaaag
     4921 actgggatcc aaaaaaatat ggtggttttg atagtccaac ggtagcttat tcagtcctag
     4981 tggttgctaa ggtggaaaaa gggaaatcga agaagttaaa atccgttaaa gagttactag
     5041 ggatcacaat tctggaaaga agttcctttg aaaaaaatcc gattgacttt ttagaagcta
     5101 aaggatataa ggaagttaaa aaagacttaa tcattaaact acctaaatat agtctttttg
     5161 agttagaaaa cggtcgtaaa cggatgctgg ctagtgccgg agaattacaa aaaggaaatg
     5221 agctggctct gccaagcaaa tatgtgaatt ttttatattt agctagtcat tatgaaaagt
     5281 tgaagggtag tccagaagat aacgaacaaa aacaattgtt tgtggagcag cataagcatt
     5341 atttagatga gattattgag caaatcagtg aattttctaa gcgtgttatt ttagcagatg
     5401 ccaatttaga taaagttctt agtgcatata acaaacatag agacaaacca atacgtgaac
     5461 aagcagaaaa tattattcat ttatttacgt tgacgaatct tggagctccc gctgctttta
     5521 aatattttga tacaacaatt gatcgtaaac gatatacgtc tacaaaagaa gttttagatg
     5581 ccactcttat ccatcaatcc atcactggtc tttatgaaac acgcattgat ttgagtcagc
     5641 taggaggtga ctaataaaag cttgatcaga tctcagcttt tttttgaagt gcctggcagt
     5701 aagttgaaaa aatttgaaat aaacttggac tatgttaata aaaaaataca aattaacgaa
     5761 caaaaagatt tgaattatta tataatttag ttaaaagcaa ttttaaatga gggaggtttc
     5821 catatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     5881 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     5941 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
     6001 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     6061 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     6121 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     6181 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     6241 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     6301 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     6361 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     6421 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     6481 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     6541 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
     6601 gtagtcggca aataatgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     6661 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6721 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6781 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6841 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6901 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6961 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     7021 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     7081 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7141 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7201 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7261 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7321 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7381 cacatgttct ttcctgcact agatccccgg caagttcatc cttagggttt tcatatctct
     7441 taaaaagtca atttctgctt gattaatttc ttgtggatta ttgtttttgc ggtttttcat
     7501 tttttatctc cttgagtatt gaattttttc ttatatataa atatatagca aaaactattt
     7561 ttgccaactt ttttacaaaa aattttttat taaaacactt agggctttgc taaattctct
     7621 tttaaaagaa cttagtaaag ccctatggat ttaagaactg attatactta cgtagtaaaa
     7681 atactacaga tattaatagt atagaccata tcaaaatcat aatcaattta tatttatcta
     7741 aaattgtagt ccatacaaaa tacaaaaaaa ttaggttttt ctaaattcta ttttaaaaga
     7801 acttagtaac acctaatctc taaattactt acaaaattct aaaattagat ttttagttct
     7861 tcatatttct ttaaaagttt tttaagaaaa tctttttgat tttcaaaaat ttcatgcatc
     7921 ataaaactag taaatttgga tttttttttg taaaaatcat aactttcttg ggttttaagt
     7981 tgaaatctta atggttttat tggattttgc ctagattttt ttaacgccgg actttcttta
     8041 tcttttataa aattataaga atttttaata ccattttcta ttaaataatc ttcttttata
     8101 agcccctctt gcagtgcagt tgctaatctt aaatacttat acgcttgagt ttttgctaca
     8161 tcaaagtctt ttataaaatc aagaaaagtt ttataaccat caaatttata atattgatta
     8221 tcttttattt cttttaaaat tttaatattt tccaccctgt ggaatatttc ttttttgaaa
     8281 ttgtcattta acttttcctt tagtgtcttg taataagaaa ttttactttc attaatcatt
     8341 aaattttgtt caacataagt atctatcttt tttgttaaaa ctttaacaac acgttttttt
     8401 tccataaaaa ttttccttta tttttgcaac attttcctta attgatataa ttgttactat
     8461 tttagatttt ttaatatcca cttaagaatt aatccctcac tagaatattt tttattatag
     8521 aattcatata catgtttttc taaatctata aatttatcaa caattgcttt attatagggt
     8581 gtatctatct tttcttttct taataatcgt ctcatattct ttaaataaca acaaacactg
     8641 ccggttttaa acctaaattc tatgtaatat atttttgaaa aagtatatac attttcaacc
     8701 ccgtttattt catactttac aaaaacatta tttattggtt ttctataacc atagtaaatg
     8761 cctaaaaatt tgtccccgtc tttcatagga tatagataaa gtttttgaat ctctgattta
     8821 tctaataatt tttcaaaaaa aattatacac cgcttttgtt tgttactaac tactctatgc
     8881 aatgccttta atatttttgt gtgatatata tttttatcac cttttttttc tttcagcagg
     8941 aagatacttt cttttggaaa tttaattttt tgttcattta ttcttgcagc cttaagtccg
     9001 attttcatat ttaatcctta tatagccttt ctatttaatt cttttaaact caataaactt
     9061 ttttggtttt tcattagttc taataattcg caataatatg tgcaaaacac tttattgtat
     9121 tctattttct tttggttatt caaatattct tttataattg gctttagaag ttcaatattt
     9181 acttcttttt ttagttgttc aataaggata ttgaaaatgt ttgtttttaa atcttgataa
     9241 ttttgttgag aattttcttt ttttctttca attgacttct ctaatttacg ctttatattg
     9301 tttaaatcgc tatatttatg actttcaata ataaaatgag gcttaaattt gtaattttcg
     9361 tatatttttt ggaaacttgt ttctaattgt ttagaattat attcattttt ttcaaattct
     9421 ttttgagtgt tgcatagaat ttcctttaat ttttcttgtt tttctttcat acaagattta
     9481 gtaaaatgta tatttttatt ttttagtaat ttaatttcta ttatttttat gattttaagt
     9541 attttgatta tattatcttt atcaatattt aaattgaata aaattgtaag aaaaattgaa
     9601 agaatttttt tacataaaaa gttacattta ttgaagtaat ttcttacttg atacttttct
     9661 atttcattaa tctttctttc ttcttttata ttatttttat tatttaaaca ctcctttaaa
     9721 tctacactcc catttttagt aaatctatct ttaaggcccc ttgaaactct attttgaaat
     9781 cttaaatctt ttttttcttg aaagtattta tagatttttt ggtaacattc tttttttgaa
     9841 aaattaagtt gatagtatat ttcggtcccc aaatttaccc ctaagtgttt gtgataatta
     9901 gttgtgactt taaattcttt ttctaatttg taaaggtaat tttgcagtgt ttttagttta
     9961 acgggtgttt gactatttct tttaaggttt tcattaaagt aatagagtat gtttttttgt
    10021 gtgtatttct tatatgtttt atttatgtac gctagtgttg agataaggac tactaattta
    10081 tactgtaatt tattgtagca agttgtggtt ttactggttt tgtttatatt agataagttt
    10141 tccatttata gctccttgaa taccggattt tttatctata aagtatatat atcacaattt
    10201 tgatttaaaa gtaaattctt tttttaaaaa tacaaaaatt ttttataaat tatatagacc
    10261 atgatattgt attaatacta attgatttta atttaaggtt gaactaaaat aaacatagtt
    10321 ttgtagaaaa taatttttca ttattttgct acttgatatt ggatcgtaaa aatattaggg
    10381 ctttacaaag ttcttttaaa agagaattta gtaaagccct aagtctttta ataaaaaatt
    10441 ttttgtaaaa aagttggcaa aaatagtttt tgctatatat ttatatataa gaaaaaattc
    10501 aatactcaag gagataaaaa atggaaaact taaacaataa tccacaaaaa attaatcaaa
    10561 taaataagca aactaatttt attggttttg aaaattttat agaacttgat aatcaagtta
    10621 atcaaactaa agaaaaaagt aaattaagta agataggtaa gaaattaccc ggaataagca
    10681 gtcaagtcta gtgcggccgg aaacagctat gacatgatta cgaattcgag ctccgaagtt
    10741 tattatttta tgatttaaat taataaaaag atttatcggt gtgaatagaa tatttctttg
    10801 gtttattatg catcctagta catattatat aatttaattt gaaaatttaa atttctgact
    10861 tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
    10921 tggcaccgag tcggtgc
//
