LOCUS       sequence-312983-         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of mKate2 under the ZF2x6-C promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Muldoon JJ, Kandula V, Hong M, Donahue PS, Boucher JD, Bagheri N, 
            Leonard JN
  TITLE     Model-guided design of mammalian genetic programs.
  JOURNAL   Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe9375. doi: 
            10.1126/sciadv.abe9375. Print 2021 Feb.
  PUBMED    33608279
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-02-19"; volume: "7(8). pii: 7/8/eabe9375. doi"; pages: "
            10.1126/sciadv.abe9375. Print 2021 Feb"
COMMENT     Sequence Label: pJM584 ZF2x6-C mKate2 in TUPV1
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..699
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
ORIGIN
        1 atggtgagcg agctgattaa ggagaacatg cacatgaagc tgtatatgga gggcaccgtg
       61 aacaaccacc acttcaagtg tacctccgag ggcgaaggca agccctacga gggcacccag
      121 accatgagaa tcaaggcggt cgagggcggc cctctcccct tcgccttcga catcctggct
      181 accagcttca tgtacggcag caaaaccttc atcaaccaca cccagggcat ccccgacttc
      241 tttaagcagt ccttccccga gggcttcaca tgggagagag tcaccacata cgaagatggg
      301 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
      361 aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc
      421 tgggaggcct ccaccgaaac cctgtacccc gctgacggcg gcctggaagg cagagccgac
      481 atggccctga agctcgtggg cgggggccac ctgatctgca acttgaaaac cacatacaga
      541 tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga caggagactg
      601 gaaagaatca aggaggccga caaagaaacc tacgtcgagc agcacgaggt ggctgtggcc
      661 agatactgcg acctccctag caaactgggg cacagatga
//
