LOCUS       sequence-31299-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBH12 (3BI7)
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             661..678
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             728..745
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(798..816)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      812..859
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttccctctga atattttgtt taccttaaga aggagatata ctatgccgtc caaccactac
       61 ggacccatcc cggggatccc cgtgggcacc atgtggcggt tccgagtcca ggtcagcgag
      121 tcgggtgtcc atcggcccca cgtggctggc atacacggcc ggagcaacga cggagcgtac
      181 tccctagtcc tggcgggggg ctatgaggat gacgtggacc atgggaattt tttcacatac
      241 acgggtagtg gtggtcgaga tctttccggc aacaagagga ccgcggaaca gtcttgtgat
      301 cagaaactca ccaacaccaa cagggcgctg gctctcaact gctttgctcc catcaatgac
      361 caagaagggg ccgaggccaa ggactggcgg tcggggaagc cggtcagggt ggtgcgcaat
      421 gtcaagggtg gcaagaatag caagtacgcc cccgctgagg gcaaccgcta tgatggcatc
      481 tacaaggttg tgaaatactg gcccgagaag gggaagtccg ggtttctcgt gtggcgctac
      541 cttctgcgga gggacgatga tgagcctggc ccttggacga aggaggggaa ggaccggatc
      601 aagaagctgg ggctgaccat gcagtatcca gaaggctacc tggaagccct ggccaacgcg
      661 caccatcatc accaccattg aggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc
      721 actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc
      781 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
      841 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg
      901 ccctgtagcg gcgcattaag cgcgc
//
