LOCUS       sequence-313-exp        6129 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6129)
  TITLE     Fire Lab C. Elegans Vector Kit 2005
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD159.88
FEATURES             Location/Qualifiers
     source          1..6129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1806..1834)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2582..2602
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(3569..3591)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3691..3710
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3788..3806)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3874..3978
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3979..4047,4048..4839)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4197..4216)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5010..5598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5499..5518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5752..5769
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5886..5907
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5922..5927,5928..5945,5946..5952)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5960..5976
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5965..5987
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5984..6000
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5984..6000
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccct cgaattctta tcatcttcgg
       61 ttttgataaa attgtggtgt agtccataat gaaataaaat tagaaacaat tgaaaatgag
      121 aataattgga aggaaagaga accggtcatt tagaatgaac acaatagctt ttttagtgat
      181 tgttgcgtaa attttatgac attacgtcat tgaatcctga agaagacaat gtttaaatgt
      241 tctccagatg gaattaaatg tataattaag ctcgagattt ttatccgagt ttttataaaa
      301 gttggtgaga cccaggatta atcaaaaaca agtattgaca gttccgtcat tcataactcc
      361 accgactgct caagttggca aaagtcaact tttaaagtgg gttataaaga gacttcgatt
      421 tttcagattt gcaaatcatt tgaaagcatt tgttaaacta gatataaata catagttttc
      481 ctcaatcagg aaaatcaaac cactgatcat ctgatcagct gtcttctaac aggatgcact
      541 attaatgaat taaacgaact cggtctattc actcggataa ttgcaacaat gttagttcta
      601 tttgatttat ttcggcgcga tccgtagcga cactttcttc cagcaaccat gtgatgctga
      661 tttctatcat ccgaagagag aaggaaacat ggagaggagc ggttcaagaa caggtgaatc
      721 tgaacctcct tttccgtgtg tgtggaaata tacacaaaca agtgactgat gaaggcggga
      781 aaaagaaaaa cgatggattg agttatctgg gtgaagaatc ttagacaaag agtttgcaga
      841 aaaagcttgt tcttggaaaa ataaaaaact agacaatttt taaaaaggaa aactacttat
      901 ttatcacttc aaaaccagaa cccttttgac aatctttaac tgtttgaact tcgtattaga
      961 tctcagcagc aaaataatag ttgaaaaaat tttataagaa aagttttcgt gttcagttga
     1021 aaacatccaa aacagaaagt atttttattt actgataatt ccttaataca catctattac
     1081 cgtatttggg gcttctaaaa atatattcta gtcgtttcaa agttcaagca cattacgtta
     1141 attcatagat caaatccact aaacctcaac catcactttt tctaatttct tccacttcaa
     1201 aaaaaaacta atcatcgtgg cttcttcccg ttccatgtca tatggtcaac attaatcagt
     1261 cgatggaatt ggccttttta ccgaaaatac gaagattcca cgcatcattg caacattttc
     1321 gtcataacct cgctagccca aatcggttgt aggatccccg aatcgcattg tggttgagcg
     1381 tatgtccttc aaaaagtcca acaaaaactc gaataccgtt tgccatcgga tggtgttgtt
     1441 gaagagtttt aactataagc tacgcccatc cggacgtacg cgagaagccc tttctgttga
     1501 atgggtgggc ggcggaagaa aaaacttctt aacacggctt ttcggttttt ctaccctata
     1561 tattggagga taggtgaagg atattttgga gaatatacga ttagcggcaa tcacaatgac
     1621 atccttatgt atatgcattc tttttattgt ttcctgatat ttattctctc tttatcattt
     1681 cactttttct tggcactcgt ccaaaaatct caattaaaaa tttctggata tatctttaga
     1741 tctaactgaa aatttccagg gtaccggtag aaaaaatgag taaaggagaa gaacttttca
     1801 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
     1861 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
     1921 ctactggaaa actacctgtt ccatgggtaa gtttaaacat atatatacta actaaccctg
     1981 attatttaaa ttttcagcca acacttgtca ctactttctg ttatggtgtt caatgcttct
     2041 cgagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt
     2101 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtaagttta
     2161 aacagttcgg tactaactaa ccatacatat ttaaattttc aggtgctgaa gtcaagtttg
     2221 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
     2281 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
     2341 acaaacaaaa gaatggaatc aaagttgtaa gtttaaacat gattttacta actaactaat
     2401 ctgatttaaa ttttcagaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
     2461 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     2521 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     2581 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
     2641 aatagcattc gtagaattcc aactgagcgc cggtcgctac cattaccaac ttgtctggtg
     2701 tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa actgagttct actaactaac
     2761 gagtaatatt taaattttca gcatctcgcg cccgtgcctc tgacttctaa gtccaattac
     2821 tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat
     2881 caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga
     2941 aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc
     3001 cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct
     3061 tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg cacacaaaat
     3121 accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc gcacgtctgg
     3181 gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt tttggaattt
     3241 ttcaatcaag tgaaagttta tgaaattaat tttcctgctt ttgctttttg gggtttcccc
     3301 tattgtttgt caagatttcg aggacggcgt ttttcttgct aaaatcacaa gtattgatga
     3361 gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga aggtgagtag
     3421 aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa tgacagaata
     3481 cattcccaat ataccaaaca taactgtttc ctactagtcg gccgtacggg ccctttcgtc
     3541 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     3601 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     3661 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     3721 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcggc
     3781 cttaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     3841 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3901 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3961 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     4021 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     4081 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     4141 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     4201 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     4261 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     4321 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     4381 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     4441 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     4501 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     4561 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     4621 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     4681 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     4741 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4801 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     4861 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     4921 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4981 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5041 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5101 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     5161 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5221 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5281 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5341 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5401 cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5461 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5521 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5581 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5641 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5701 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     5761 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     5821 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     5881 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     5941 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     6001 catgattacg ccaagctgta agtttaaaca tgatcttact aactaactat tctcatttaa
     6061 attttcagag cttaaaaatg gctgaaatca ctcacaacga tggatacgct aacaacttgg
     6121 aaatgaaat
//
