LOCUS       sequence-31300-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBH12 (3CLZ)
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             742..759
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(812..830)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      826..873
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aattcctctg aatattttgt ttactttaag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggttcc gcgtggtagt ccgtccaacc actacggacc
      121 catcccgggg atccccgtgg gcaccatgtg gcggttccga gtccaggtca gcgagtcggg
      181 tgtccatcgg ccccacgtgg ctggcataca cggccggagc aacgacggag cgtactccct
      241 agtcctggcg gggggctatg aggatgacgt ggaccatggg aattttttca catacacggg
      301 tagtggtggt cgagatcttt ccggcaacaa gaggaccgcg gaacagtctt gtgatcagaa
      361 actcaccaac accaacaggg cgctggctct caactgcttt gctcccatca atgaccaaga
      421 aggggccgag gccaaggact ggcggtcggg gaagccggtc agggtggtgc gcaatgtcaa
      481 gggtggcaag aatagcaagt acgcccccgc tgagggcaac cgctatgatg gcatctacaa
      541 ggttgtgaaa tactggcccg agaaggggaa gtccgggttt ctcgtgtggc gctaccttct
      601 gcggagggac gatgatgagc ctggccattg gacgaaggag gggaaggacc ggatcaagaa
      661 gctggggctg accatgcagt atccagaagg ctacctggaa gccctggcca actgatgacg
      721 aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa
      781 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
      841 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg
      901 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
//
