LOCUS       sequence-313006-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of 3xFLAG-NLS-FKBP-ZF2 under the CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Muldoon JJ, Kandula V, Hong M, Donahue PS, Boucher JD, Bagheri N, 
            Leonard JN
  TITLE     Model-guided design of mammalian genetic programs.
  JOURNAL   Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe9375. doi: 
            10.1126/sciadv.abe9375. Print 2021 Feb.
  PUBMED    33608279
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-02-19"; volume: "7(8). pii: 7/8/eabe9375. doi"; pages: "
            10.1126/sciadv.abe9375. Print 2021 Feb"
COMMENT     Sequence Label: pJB001 CMV 3xFLAG-NLS-FKBP-ZF2
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(139..141,142..462)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
ORIGIN
        1 atggattata aggatcacga tggcgactac aaggaccacg atattgacta caaagacgat
       61 gacgataaaa tggctcctaa gaaaaagcgc aaagtcggta tccatggcgt tcccgtcgac
      121 ggtggactcc aagttaagat gggagtgcag gtggaaacca tctccccagg agacgggcgc
      181 accttcccca agcgcggcca gacctgcgtg gtgcactaca ccgggatgct tgaagatgga
      241 aagaaatttg attcctcccg ggacagaaac aagcccttta agtttatgct aggcaagcag
      301 gaggtgatcc gaggctggga agaaggggtt gcccagatga gtgtgggtca gagagccaaa
      361 ctgactatat ctccagatta tgcctatggt gccactgggc acccaggcat catcccacca
      421 catgccactc tcgtctttga tgtggagctt ctaaaactgg aaggatccgg tggtggcggg
      481 tctggaggag gaggtagtgg tggaggtggc tctggtaccg gtggagctag acccggggag
      541 cgccccttcc agtgtcgcat ttgcatgcgg aacttttcga gaaatttcat tttgcaaaga
      601 catacccgta ctcataccgg tgaaaaaccg tttcagtgtc ggatctgtat gcgaaatttc
      661 tccgatagag ctaatttgag gaggcatctg cgtacgcaca ccggcgagaa gccattccaa
      721 tgccgaatat gcatgcgcaa cttcagtaga catgatcaat tgactagaca cctaaaaacc
      781 cacctgagag gatcttaa
//
