LOCUS       sequence-313008-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of 3xFLAG-NLS-VP16-FRB under the ZF1/2x6-C 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Muldoon JJ, Kandula V, Hong M, Donahue PS, Boucher JD, Bagheri N, 
            Leonard JN
  TITLE     Model-guided design of mammalian genetic programs.
  JOURNAL   Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe9375. doi: 
            10.1126/sciadv.abe9375. Print 2021 Feb.
  PUBMED    33608279
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-02-19"; volume: "7(8). pii: 7/8/eabe9375. doi"; pages: "
            10.1126/sciadv.abe9375. Print 2021 Feb"
COMMENT     Sequence Label: pJB003 ZF1/2x6-C 3xFLAG-NLS-VP16-FRB
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggattata aggatcacga tggcgactac aaggaccacg atattgacta caaagacgat
       61 gacgataaaa tggctcctaa gaaaaagcgc aaagtcggta tccatggcgt tcccgtcgac
      121 ggtggactcc aagccccacc aaccgatgtt tctttaggtg atgagttaca tttggacggt
      181 gaggacgtag ctatggcaca tgcagatgcc ttagacgatt ttgacttgga tatgttagga
      241 gatggagatt caccaggacc tggtttcaca ccacacgatt cagctccata cggtgctttg
      301 gacatggccg atttcgaatt tgaacagatg tttactgatg ccttgggtat agacgaatac
      361 ggtggtggag gatccggtgg tggcgggtct ggaggaggag gtagtggtgg aggtggctct
      421 ggtaccatgc ttgagatgtg gcatgaaggc ctggaagagg catctcgttt gtactttggg
      481 gaaaggaacg tgaaaggcat gtttgaggtg ctggagccct tgcatgctat gatggaacgg
      541 ggaccccaga ctctgaagga aacatccttt aatcaggcct atggtcgaga tttaatggag
      601 gcccaagagt ggtgcaggaa gtacatgaaa tcagggaatg tcaaggacct cctccaagcc
      661 tgggacctct attatcatgt gttccgacga atctcaaaga ctagatga
//
