LOCUS       sequence-313009-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of 3xFLAG-NES-PYL1-VP64 under the CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Muldoon JJ, Kandula V, Hong M, Donahue PS, Boucher JD, Bagheri N, 
            Leonard JN
  TITLE     Model-guided design of mammalian genetic programs.
  JOURNAL   Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe9375. doi: 
            10.1126/sciadv.abe9375. Print 2021 Feb.
  PUBMED    33608279
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-02-19"; volume: "7(8). pii: 7/8/eabe9375. doi"; pages: "
            10.1126/sciadv.abe9375. Print 2021 Feb"
COMMENT     Sequence Label: pPD1122 CMV 3xFLAG-NES-PYL1-VP64 in TUPV3
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             733..882
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 atggactaca aagaccacga cggcgattac aaggatcatg acatcgatta caaagacgat
       61 gacgataaga tggaagagct gagccaggct ctggccagct cctttagcgt gtcctccgga
      121 atgggcggag gagcccctac ccaggacgag ttcacccagc tgagccagag catcgctgag
      181 ttccacacct accagctggg aaacggacgc tgttccagcc tgctggcaca gagaatccac
      241 gctcctcctg agacagtgtg gagtgtggtg cgcagattcg accgccctca gatttacaag
      301 cacttcatca agagctgcaa cgtgagcgag gacttcgaga tgagagtggg atgtaccaga
      361 gatgtgaacg tgatcagcgg actgcctgcc aacaccagca gagagagact ggacctgctg
      421 gacgatgacc gcagagtgac cggcttcagc atcaccggag gtgagcacag actgagaaac
      481 tacaagagcg tgaccaccgt ccaccgcttc gagaaggaag aggaagagga gcgcatctgg
      541 accgtggtgc tggagagcta cgtcgtggac gtgcccgagg gcaacagcga agaggatacc
      601 cgcctgttcg ctgacaccgt gatcagactg aacctccaga agctggccag catcaccgag
      661 gcaatgaacg gatccggtgg tggcgggtct ggaggaggag gtagtggtgg aggtggctct
      721 ggtaccgccg gagacgctct ggatgacttt gatctggaca tgcttggttc cgatgccttg
      781 gacgattttg acctcgatat gctgggctcc gacgctctcg acgatttcga cctcgacatg
      841 ctgggtagcg atgcactgga tgactttgat ctggatatgc tcggatcata g
//
