LOCUS       sequence-31301-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NFATC2IP
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             360..377
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(430..448)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      444..491
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      528..908
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(615..634)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     825..846
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttccctctga atattttgtt tactttaaga aggagatata ccatgcatca tcatcatcat
       61 cacagcagcg gcagagaaaa cttgtatttc cagggcacag agacgtccca acagctccag
      121 ctccgggtgc agggaaagga gaaacaccag acactggaag tctcactgtc tcgagattcc
      181 cctctaaaga ccctcatgtc ccactatgag gaggccatgg gactgtcggg acggaagctc
      241 tccttcttct ttgatgggac aaagctttca ggcagggagc tgccagctga cctgggcatg
      301 gaatctgggg acctcattga ggtctggggc tgatgacgaa gcttgcggcc gcactcgagc
      361 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      421 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
      481 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
      541 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      601 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      661 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
      721 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
      781 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
      841 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
      901 gatttcggcc ta
//
