LOCUS       sequence-313010-        1335 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of 3xFLAG-NLS-ZF2-ABI1 under the CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Muldoon JJ, Kandula V, Hong M, Donahue PS, Boucher JD, Bagheri N, 
            Leonard JN
  TITLE     Model-guided design of mammalian genetic programs.
  JOURNAL   Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe9375. doi: 
            10.1126/sciadv.abe9375. Print 2021 Feb.
  PUBMED    33608279
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-02-19"; volume: "7(8). pii: 7/8/eabe9375. doi"; pages: "
            10.1126/sciadv.abe9375. Print 2021 Feb"
COMMENT     Sequence Label: pKD011 CMV 3xFLAG-NLS-ZF2-ABI1 in TUPV2
FEATURES             Location/Qualifiers
     source          1..1335
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggactaca aagaccacga cggcgattac aaggatcatg acatcgatta caaagacgat
       61 gacgataaga tggctcctaa gaaaaagcgc aaagtctccg gagctagacc cggggagcgc
      121 cccttccagt gtcgcatttg catgcggaac ttttcgagaa atttcatttt gcaaagacat
      181 acccgtactc ataccggtga aaaaccgttt cagtgtcgga tctgtatgcg aaatttctcc
      241 gatagagcta atttgaggag gcatctgcgt acgcacaccg gcgagaagcc attccaatgc
      301 cgaatatgca tgcgcaactt cagtagacat gatcaattga ctagacacct aaaaacccac
      361 ctgagaggat ctggatccgg tggtggcggg tctggaggag gaggtagtgg tggaggtggc
      421 tctggtacca tgacgcgggt gcctttgtat ggttttactt cgatttgtgg aagaaggcct
      481 gagatggaag ctgctgtttc gactatacca agattccttc aatcttcctc tggttcgatg
      541 ttagatggtc ggtttgatcc tcaatccgcc gctcatttct tcggtgttta cgacggccat
      601 ggcggttctc aggtagcgaa ctattgtaga gagaggatgc atttggcttt ggcggaggag
      661 atagctaagg agaaaccgat gctctgcgat ggtgatacgt ggctggagaa gtggaagaaa
      721 gctcttttca actcgttcct gagagttgac tcggagattg agtcagttgc gccggagacg
      781 gttgggtcaa cgtcggtggt tgccgttgtt ttcccgtctc acatcttcgt cgctaactgc
      841 ggtgactcta gagccgttct ttgccgcggc aaaactgcac ttccattatc cgttgaccat
      901 aaaccggata gagaagatga agctgcgagg attgaagccg caggagggaa agtgattcag
      961 tggaatggag ctcgtgtttt cggtgttctc gccatgtcga gatccattgg cgatagatac
     1021 ttgaaaccat ccatcattcc tgatcctgaa gtgacggctg tgaagagagt aaaagaagat
     1081 gattgtctga ttttggcgag tgacggggtt tgggatgtaa tgacggatga agaagcgtgt
     1141 gagatggcaa ggaagcggat tctcttgtgg cacaagaaaa acgcggtggc tggggatgca
     1201 tcgttgctcg cggatgagcg gagaaaggaa gggaaagatc ctgcggcgat gtccgcggct
     1261 gagtatttgt caaagctggc gatacagaga ggaagcaaag acaacataag tgtggtggtg
     1321 gttgatttga agtag
//
