LOCUS       sequence-313012-         579 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of 3xFLAG-NLS-intC-ZF2 under the CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Muldoon JJ, Kandula V, Hong M, Donahue PS, Boucher JD, Bagheri N, 
            Leonard JN
  TITLE     Model-guided design of mammalian genetic programs.
  JOURNAL   Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe9375. doi: 
            10.1126/sciadv.abe9375. Print 2021 Feb.
  PUBMED    33608279
REFERENCE   2  (bases 1 to 579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-02-19"; volume: "7(8). pii: 7/8/eabe9375. doi"; pages: "
            10.1126/sciadv.abe9375. Print 2021 Feb"
COMMENT     Sequence Label: pJB005 CMV 3xFLAG-NLS-intC-ZF2
FEATURES             Location/Qualifiers
     source          1..579
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggattata aggatcacga tggcgactac aaggaccacg atattgacta caaagacgat
       61 gacgataaaa tggctcctaa gaaaaagcgc aaagtcggta tccatggcgt tcccggatca
      121 ggctccggaa tgatgctgaa gaagatcctg aagatcgagg agctggacga gagagagctg
      181 atcgacatcg aggtgagcgg caaccacctg ttctacgcca acgacatcct gacccacaac
      241 agcaacaggg gtggtggcgg gtctggtgga ggaggtagtg gtggaggtgg ctctggtacc
      301 ggtggagcta gacccgggga gcgccccttc cagtgtcgca tttgcatgcg gaacttttcg
      361 agaaatttca ttttgcaaag acatacccgt actcataccg gtgaaaaacc gtttcagtgt
      421 cggatctgta tgcgaaattt ctccgataga gctaatttga ggaggcatct gcgtacgcac
      481 accggcgaga agccattcca atgccgaata tgcatgcgca acttcagtag acatgatcaa
      541 ttgactagac acctaaaaac ccacctgaga ggatcttaa
//
