LOCUS       sequence-313018-         597 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of 3xFLAG-NLS-VP64-intN under the EF1-alpha 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Muldoon JJ, Kandula V, Hong M, Donahue PS, Boucher JD, Bagheri N, 
            Leonard JN
  TITLE     Model-guided design of mammalian genetic programs.
  JOURNAL   Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe9375. doi: 
            10.1126/sciadv.abe9375. Print 2021 Feb.
  PUBMED    33608279
REFERENCE   2  (bases 1 to 597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-02-19"; volume: "7(8). pii: 7/8/eabe9375. doi"; pages: "
            10.1126/sciadv.abe9375. Print 2021 Feb"
COMMENT     Sequence Label: pJM554 EF1-alpha 3xFLAG-NLS-VP64-intN in TUPV2
FEATURES             Location/Qualifiers
     source          1..597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             112..261
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 atggactata aagaccatga cggtgactac aaggatcacg acatagatta taaagacgat
       61 gacgacaaga tggcacctaa gaagaagcgc aaagttggct ctggcgaatt cgacgccctt
      121 gatgactttg acttggacat gctggggtca gacgcactcg atgattttga ccttgatatg
      181 ctgggaagcg atgcacttga tgactttgac ttggacatgc tcggctccga cgctcttgat
      241 gatttcgatc ttgacatgct cggatccggt ggtggcgggt ctggaggagg aggtactggt
      301 ggaggtggca ctggtaccag aagcggatac tgcctggacc tgaagaccca ggtgcagacc
      361 cctcagggca tgaaggagat cagcaacatc caggtgggcg acctggtgct gagcaacacc
      421 ggctacaacg aggtgctgaa cgtgttcccc aagagcaaga agaagagcta caagatcacc
      481 ctggaggacg gcaaggagat catctgcagc gaggagcacc tgttccccac ccagaccggc
      541 gagatgaaca tcagcggcgg cctgaaggag ggcatgtgcc tgtacgtgaa ggagtga
//
