LOCUS       sequence-31302-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBI-D4
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             695..712
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(765..783)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      779..826
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaattcctct gaatattttg tttactttaa gaaggagata taccatgcat catcatcatc
       61 atcacagcag cggcagagaa aacttgtatt tccagggcga ggaccgggat aagccctatg
      121 cctgtgacat ttgtggaaaa cgttacaaga accgaccagg cctcagttac cactatgccc
      181 actcccactt ggctgaggag gagggcgagg acaaggaaga ctctcaacca cccactcctg
      241 tttcccagag gtctgaggag cagaaatcca aaaagggtcc tgatggattg gccttgccca
      301 acaactactg tgacttctgc ctgggggact caaagattaa caagaagacg ggacaacccg
      361 aggagctggt gtcctgttct gactgtggcc gctcagggca tccatcttgc ctccaattta
      421 cccccgtgat gatggcggca gtgaagacat accgctggca gtgcatcgag tgcaaatgtt
      481 gcaatatctg cggcacctcc gagaatgacg accagttgct cttctgtgat gactgcgatc
      541 gtggctacca catgtactgt ctcaccccgt ccatgtctga gccccctgaa ggaagttgga
      601 gctgccacct gtgtctggac ctgttgaaag agaaagcttc catctaccag aaccagaact
      661 cctcttgatg acgaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc
      721 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
      781 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
      841 tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      901 gtggttacgc gcagcgtgac cgctacactt gccagcgcta
//
