LOCUS       sequence-31303-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: USP04
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             817..834
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggttcc gcgtggtagt atggcggaag gccggggcag
      121 ccgtgagcga ccggatgtgg agacccagaa gacggagctc ggagccttga tggggaccac
      181 gctccaacgt ggggcgcagt ggtatcttat tgacagccgg tggttcaagc agtggaagaa
      241 gtatgtgggc tttgacagct gggacatgta caatgtcggg gagcataacc tgtttcctgg
      301 accgattgac aactccggac tcttctcaga tcctgagagt cagaccttga aggagcactt
      361 aatcgatgag ctggactatg tgctggtccc agccgaagcc tggaataaat tgctgaattg
      421 gtatggctgt gtggagggtc agcagcctat tgtcagaaaa gttgtggagc atggcctgtt
      481 tgtcaagcac tgcaaagtgg aagtgtattt gctggagctg aagctctgtg agaacagtga
      541 ccccaccaat gtgctaagtt gccattttag caaagcagac accattgcaa ctattgagaa
      601 ggagatgagg aagctcttca acatccctgc agaacgtgaa acacggcttt ggaacaaata
      661 catgagcaac acctatgagc agttgagcaa gctagacaac actatccagg atgctgggct
      721 gtaccagggt caggtgctag taattgagcc ccaaaatgaa gatggcacat ggccccggca
      781 gagcctgtga tgacgaagct tgcggccgca ctcgagcacc accaccacca ccactgagat
      841 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tga
//
