LOCUS       sequence-313033-         648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of intN-ZF10-NLS-HA under the EF1-alpha 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Muldoon JJ, Kandula V, Hong M, Donahue PS, Boucher JD, Bagheri N, 
            Leonard JN
  TITLE     Model-guided design of mammalian genetic programs.
  JOURNAL   Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe9375. doi: 
            10.1126/sciadv.abe9375. Print 2021 Feb.
  PUBMED    33608279
REFERENCE   2  (bases 1 to 648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-02-19"; volume: "7(8). pii: 7/8/eabe9375. doi"; pages: "
            10.1126/sciadv.abe9375. Print 2021 Feb"
COMMENT     Sequence Label: pJM589 EF1-alpha intN-ZF10-NLS-HA in TUPV2
FEATURES             Location/Qualifiers
     source          1..648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             592..612
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             619..645
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atgaccagaa gcggatactg cctggacctg aagacccagg tgcagacccc tcagggcatg
       61 aaggagatca gcaacatcca ggtgggcgac ctggtgctga gcaacaccgg ctacaacgag
      121 gtgctgaacg tgttccccaa gagcaagaag aagagctaca agatcaccct ggaggacggc
      181 aaggagatca tctgcagcga ggagcacctg ttccccaccc agaccggcga gatgaacatc
      241 agcggcggcc tgaaggaggg catgtgcctg tacgtgaagg agactggttc tggttctggt
      301 tcaggatccg gtaccggtgg agctagaccc ggggagcgcc ccttccagtg tcgcatttgc
      361 atgcggaact tttcgactaa tcaaaaattg gaagttcata cccgtactca taccggtgaa
      421 aaaccgtttc agtgtcggat ctgtatgcga aatttctccg ttagacataa tttgcaaaga
      481 catctacgta cgcacaccgg cgagaagcca ttccaatgcc gaatatgcat gcgcaacttc
      541 agtcaacatc caaatttgac tagacaccta aaaacccacc tgagaggatc tcctaagaag
      601 aagcgcaaag ttggcatgta cccatacgac gtaccagatt acgcttag
//
