LOCUS       sequence-313034-        1167 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of 3xFLAG-NLS-VP64-ZF1-intC-ZF10-NLS-HA 
            under the EF1-alpha promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Muldoon JJ, Kandula V, Hong M, Donahue PS, Boucher JD, Bagheri N, 
            Leonard JN
  TITLE     Model-guided design of mammalian genetic programs.
  JOURNAL   Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe9375. doi: 
            10.1126/sciadv.abe9375. Print 2021 Feb.
  PUBMED    33608279
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-02-19"; volume: "7(8). pii: 7/8/eabe9375. doi"; pages: "
            10.1126/sciadv.abe9375. Print 2021 Feb"
COMMENT     Sequence Label: pJM590 EF1-alpha 
            3xFLAG-NLS-VP64-ZF1-intC-ZF10-NLS-...
FEATURES             Location/Qualifiers
     source          1..1167
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             121..270
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             1105..1125
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1138..1164
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atggattata aggatcacga tggcgactac aaggaccacg atattgacta caaagacgat
       61 gacgataaaa tggctcctaa gaaaaagcgc aaagtcggta tccatggcgt tcccggatca
      121 gacgcattgg acgattttga tctggatatg ctgggaagtg acgccctcga tgattttgac
      181 cttgacatgc ttggttcgga tgcccttgat gactttgacc tcgacatgct cggcagtgac
      241 gcccttgatg atttcgacct ggacatgctg attaactcta gaagttccgg atcttctggt
      301 tctggttctg gttcaggatc cggtaccggt ggagctagac ccggggagcg ccccttccag
      361 tgtcgcattt gcatgcggaa cttttcgcgc caggacaggc ttgacaggca tacccgtact
      421 cataccggtg aaaaaccgtt tcagtgtcgg atctgtatgc gaaatttctc ccagaaggag
      481 cacttggcgg ggcatctacg tacgcacacc ggcgagaagc cattccaatg ccgaatatgc
      541 atgcgcaact tcagtcgccg cgacaacctg aaccggcacc taaaaaccca cctgagagga
      601 tctggaggcg gaagcggagg cggtggatca ggctccggaa gtggctccat gatgctgaag
      661 aagatcctga agatcgagga gctggacgag agagagctga tcgacatcga ggtgagcggc
      721 aaccacctgt tctacgccaa cgacatcctg acccacaaca gcggatccgg tggtggcggg
      781 tctggaggag gaggtagtgg tggaggtggc tctggtaccg gtggagctag acccggggag
      841 cgccccttcc agtgtcgcat ttgcatgcgg aacttttcga ctaatcaaaa attggaagtt
      901 catacccgta ctcataccgg tgaaaaaccg tttcagtgtc ggatctgtat gcgaaatttc
      961 tccgttagac ataatttgca aagacatcta cgtacgcaca ccggcgagaa gccattccaa
     1021 tgccgaatat gcatgcgcaa cttcagtcaa catccaaatt tgactagaca cctaaaaacc
     1081 cacctgagag gatctggctc tggccctaag aagaagcgca aagttggctc tggcatgtac
     1141 ccatacgacg taccagatta cgcttag
//
