LOCUS       sequence-313039-        1428 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of PC 3xFLAG-FKBP-FGFR4-TEVp under the 
            EF1-alpha promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Muldoon JJ, Kandula V, Hong M, Donahue PS, Boucher JD, Bagheri N, 
            Leonard JN
  TITLE     Model-guided design of mammalian genetic programs.
  JOURNAL   Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe9375. doi: 
            10.1126/sciadv.abe9375. Print 2021 Feb.
  PUBMED    33608279
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-02-19"; volume: "7(8). pii: 7/8/eabe9375. doi"; pages: "
            10.1126/sciadv.abe9375. Print 2021 Feb"
COMMENT     Sequence Label: pJM600 EF1-alpha PC 3xFLAG-FKBP-FGFR4-TEVp in TUPV2
FEATURES             Location/Qualifiers
     source          1..1428
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..144
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(145..147,148..468)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     misc_feature    530..582
                     /label=MCS
                     /note="multiple cloning site"
     CDS             721..1425
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="ESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNKH
                     LFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQR
                     EERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFIV
                     GIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPEE
                     PFQPVKEATQLMN"
ORIGIN
        1 atgtgccgag ccatctctct taggcgcttg ctgctgctgc tgctgcagct gtcacaactc
       61 ctagctgtca ctcaagggga ttacaaggat cacgatgggg attacaaaga tcatgacatt
      121 gactacaagg atgacgatga taagatggga gtgcaggtgg aaaccatctc cccaggagac
      181 gggcgcacct tccccaagcg cggccagacc tgcgtggtgc actacaccgg gatgcttgaa
      241 gatggaaaga aatttgattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc
      301 aagcaggagg tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga
      361 gccaaactga ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc
      421 ccaccacatg ccactctcgt cttcgatgtg gagcttctaa aactggaaag tgctggtggt
      481 agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg ctggtggtcc tcgagctcaa
      541 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc tagaggcgga
      601 tctggaggct ctgggggttc aggcggatca ggaggcagtg gcgggtcaac cggtattata
      661 ctgtatgcta gtggcagtct ggcattggca gtcctcctgc tgctcgccgg gttggagaac
      721 gagagcttgt ttaaggggcc gcgtgattac aacccgatat cgagcaccat ttgtcatttg
      781 acgaatgaat ctgatgggca cacaacatcg ttgtatggta ttggatttgg tcccttcatc
      841 attacaaaca agcacttgtt tagaagaaat aatggaacac tgttggtcca atcactacat
      901 ggtgtattca aggtcaagaa caccacgact ttgcaacaac acctcattga tgggagggac
      961 atgataatta ttcgcatgcc taaggatttc ccaccatttc ctcaaaagct gaaatttaga
     1021 gagccacaaa gggaagagcg catatgtctt gtgacaacca acttccaaac taagagcatg
     1081 tctagcatgg tgtcagacac tagttgcaca ttcccttcat ctgatggcat attctggaag
     1141 cattggattc aaaccaagga tgggcagtgt ggcagtccat tagtatcaac tagagatggg
     1201 ttcattgttg gtatacactc agcatcgaat ttcaccaaca caaacaatta tttcacaagc
     1261 gtgccgaaaa acttcatgga attgttgaca aatcaggagg cgcagcagtg ggttagtggt
     1321 tggcgattaa atgctgactc agtattgtgg gggggccata aagttttcat ggtgaaacct
     1381 gaagagcctt ttcagccagt taaggaagcg actcaactca tgaattag
//
