LOCUS       sequence-31304-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: USP08 (2A9U)
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..97
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             553..570
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(623..641)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      637..684
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(808..827)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccctctgaat attttgttta ctttaagaag gagatatacc atgggcagca gccatcatca
       61 tcatcatcac agcagcggcc tggttccgcg tggtagtatg cctgctgtgg cttcagttcc
      121 taaagaactc tacctcagtt cttcactaaa agaccttaat aagaagacag aagttaaacc
      181 agagaaaata agcactaaga gttatgtgca cagtgccctg aagatcttta agacagcaga
      241 agaatgcaga ttagatcgtg atgaggaaag ggcctatgta ctatatatga aatacgtgac
      301 tgtttataat cttatcaaaa aaagacctga tttcaagcaa cagcaggatt atttccattc
      361 aatacttgga cctggaaaca tcaaaaaagc tgtcgaagaa gctgaaagac tctctgaaag
      421 ccttaaatta agatatgaag aagctgaagt ccggaaaaaa cttgaggaaa aagacaggca
      481 ggaggaagca cagcggctac aacaaaaaag gcaggaaaca ggatgatgac gaagcttgcg
      541 gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
      601 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
      661 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg
      721 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      781 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      841 cgttcgcggg ttttcccccg tc
//
