LOCUS       sequence-313040-        1101 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of TC 
            3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF1 under the EF1-alpha 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Muldoon JJ, Kandula V, Hong M, Donahue PS, Boucher JD, Bagheri N, 
            Leonard JN
  TITLE     Model-guided design of mammalian genetic programs.
  JOURNAL   Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe9375. doi: 
            10.1126/sciadv.abe9375. Print 2021 Feb.
  PUBMED    33608279
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-02-19"; volume: "7(8). pii: 7/8/eabe9375. doi"; pages: "
            10.1126/sciadv.abe9375. Print 2021 Feb"
COMMENT     Sequence Label: pJM612 EF1-alpha TC
            3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NL...
FEATURES             Location/Qualifiers
     source          1..1101
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..144
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             544..564
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             571..591
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             616..765
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 atgtgccgag ccatctctct taggcgcttg ctgctgctgc tgctgcagct gtcacaactc
       61 ctagctgtca ctcaagggga ttacaaggat cacgatgggg attacaaaga tcatgacatt
      121 gactacaagg atgacgatga taagatgctc gagatgtggc atgaaggcct ggaagaggca
      181 tctcgtttgt actttgggga aaggaacgtg aaaggcatgt ttgaggtgct ggagcccttg
      241 catgctatga tggaacgggg cccccagact ctgaaggaaa catcctttaa tcaggcctat
      301 ggtcgagatt taatggaggc ccaagagtgg tgcaggaagt acatgaaatc agggaatgtc
      361 aaggacctcc tccaagcctg ggacctctat tatcatgtgt tccgacgaat ctcaaagact
      421 agaggaggat ctggaggctc tgggggttca ggcggatcag gaggcagtgg cgggtcaacc
      481 ggtattatac tgtatgctag tggcagtctg gcattggcag tcctcctgct gctcgccggg
      541 ttggagaacc tgtattttca gatgggatct cctaagaaaa agcgcaaagt cggtatccat
      601 ggcgttcccg gatcagacgc attggacgat tttgatctgg atatgctggg aagtgacgcc
      661 ctcgatgatt ttgaccttga catgcttggt tcggatgccc ttgatgactt tgacctcgac
      721 atgctcggca gtgacgccct tgatgatttc gacctggaca tgctgattaa ctctagaagt
      781 tccggatctt ctggttctgg ttctggttca ggatccggta ccggtggagc tagacccggg
      841 gagcgcccct tccagtgtcg catttgcatg cggaactttt cgcgccagga caggcttgac
      901 aggcataccc gtactcatac cggtgaaaaa ccgtttcagt gtcggatctg tatgcgaaat
      961 ttctcccaga aggagcactt ggcggggcat ctacgtacgc acaccggcga gaagccattc
     1021 caatgccgaa tatgcatgcg caacttcagt cgccgcgaca acctgaaccg gcacctaaaa
     1081 acccacctga gaggatctta a
//
