LOCUS       sequence-313041-        1620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of TC 
            3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-DsDed-ZF1 under the EF1-alpha 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Muldoon JJ, Kandula V, Hong M, Donahue PS, Boucher JD, Bagheri N, 
            Leonard JN
  TITLE     Model-guided design of mammalian genetic programs.
  JOURNAL   Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe9375. doi: 
            10.1126/sciadv.abe9375. Print 2021 Feb.
  PUBMED    33608279
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-02-19"; volume: "7(8). pii: 7/8/eabe9375. doi"; pages: "
            10.1126/sciadv.abe9375. Print 2021 Feb"
COMMENT     Sequence Label: pJM621 EF1-alpha TC
            3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NL...
FEATURES             Location/Qualifiers
     source          1..1620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..144
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             544..564
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             571..591
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             634..1308
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWEKVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(814..834)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1226..1249
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atgtgccgag ccatctctct taggcgcttg ctgctgctgc tgctgcagct gtcacaactc
       61 ctagctgtca ctcaagggga ttacaaggat cacgatgggg attacaaaga tcatgacatt
      121 gactacaagg atgacgatga taagatgctc gagatgtggc atgaaggcct ggaagaggca
      181 tctcgtttgt actttgggga aaggaacgtg aaaggcatgt ttgaggtgct ggagcccttg
      241 catgctatga tggaacgggg cccccagact ctgaaggaaa catcctttaa tcaggcctat
      301 ggtcgagatt taatggaggc ccaagagtgg tgcaggaagt acatgaaatc agggaatgtc
      361 aaggacctcc tccaagcctg ggacctctat tatcatgtgt tccgacgaat ctcaaagact
      421 agaggaggat ctggaggctc tgggggttca ggcggatcag gaggcagtgg cgggtcaacc
      481 ggtattatac tgtatgctag tggcagtctg gcattggcag tcctcctgct gctcgccggg
      541 ttggagaacc tgtattttca gatgggatct cctaagaaaa agcgcaaagt cggtatccat
      601 ggcgttcccg gatcaggctc cggaagtggc tccatggata gcactgagaa cgtcatcaag
      661 cccttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      721 gagggcgagg gcgagggcaa gccctacgag ggcacccaga ccgccaagct ccaggtgacc
      781 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca gtacggctcc
      841 aaggtgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc cttccccgag
      901 ggcttcaagt gggagaaggt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      961 gactcctccc tccaggacgg caccttcatc taccacgtga agttcatcgg cgtgaacttc
     1021 ccctccgacg gccccgtaat gcagaagaaa actctgggct gggagccctc caccgagcgc
     1081 ctgtaccccc gcgacggcgt gctgaagggc gagatccaca aggcgctgaa gctgaagggc
     1141 ggcggccact acctggtgga gttcaagtca atctacatgg ccaagaagcc cgtgaagctg
     1201 cccggctact actacgtgga ctccaagctg gacatcacct cccacaacga ggactacacc
     1261 gtggtggagc agtacgagcg cgccgaggcc cgccaccacc tgttccagtc tggttctggt
     1321 tctggttcag gatccggtac cggtggagct agacccgggg agcgcccctt ccagtgtcgc
     1381 atttgcatgc ggaacttttc gcgccaggac aggcttgaca ggcatacccg tactcatacc
     1441 ggtgaaaaac cgtttcagtg tcggatctgt atgcgaaatt tctcccagaa ggagcacttg
     1501 gcggggcatc tacgtacgca caccggcgag aagccattcc aatgccgaat atgcatgcgc
     1561 aacttcagtc gccgcgacaa cctgaaccgg cacctaaaaa cccacctgag aggatcttaa
//
