LOCUS       sequence-313044-       21481 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human INS targeting vector for knockin of 2A separated luciferase, 
            humand CD19, and GFP. Targeted cells will be puromycin resistant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21481)
  AUTHORS   Ma H, Jeppesen JF, Jaenisch R
  TITLE     Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into
            Mechanisms of Human CD19-Positive beta Cell Destruction.
  JOURNAL   Cell Rep Med. 2020 Sep 22;1(6):100097. doi: 
            10.1016/j.xcrm.2020.100097. eCollection 2020 Sep 22.
  PUBMED    33205073
REFERENCE   2  (bases 1 to 21481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Med."; date: "2020-09-22"; volume: "1(6):100097. doi"; pages: " 
            10.1016/j.xcrm.2020.100097. eCollection 2020 Sep 22"
COMMENT     Sequence Label: INS-2A-luciferase-2A-CD19-GFP
FEATURES             Location/Qualifiers
     source          1..21481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    15..136
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(52..71)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     106..125
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    181..214
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        255..634
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        636..913
                     /label=chicken beta-actin promoter
     intron          914..1930
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1854..1876
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1938..1957
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1991..2590
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1991..2010)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2487..2507
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2607..2626
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2769..2786)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2775..2999
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3056..3089
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        7151..7213
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     5'UTR           7235..7274
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             7292..8422
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMIQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(8420..8439)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8464..8512
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        complement(9086..9104)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9125..9141)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9125..9141)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9138..9160)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9149..9165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9173..9179,9180..9197,9198..9203))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9218..9239
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9356..9373)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9527..10115)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9607..9626)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10286..11077,11078..11146))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10909..10928
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11147..11251)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(11438..11542)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11569..12024
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11656..11675)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11866..11887
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12150..12172
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12164..12181
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12165..12181
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12188..12207
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12188..12206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      15063..15072
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     16097..16168
                     /codon_start=1
                     /product="signal sequence of human preproinsulin"
                     /label=insulin signal sequence
                     /translation="MALWMRLLPLLALLALWGPDPAAA"
     CDS             17220..17246
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             17256..17309
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             17310..18959
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTLVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(17376..17394)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             18966..19019
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             20703..20756
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(20757..20759,20760..21473)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(20757..20759,20760..21470)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(20799..20820)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(21060..21079)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
       61 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      121 tcatcaatgt atcttaaaat tcctgcagcc caattccgat catattcaat aacccttaat
      181 ataacttcgt ataatgtatg ctatacgaag ttattaggtc tgaagaggag tttacgtcca
      241 gccaagctag cctcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
      301 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      361 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      421 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      481 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      541 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      601 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
      661 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
      721 attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg
      781 gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc
      841 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag
      901 cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc
      961 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga
     1021 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct
     1081 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc
     1141 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg
     1201 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg
     1261 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg
     1321 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca
     1381 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct
     1441 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt
     1501 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc
     1561 ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc
     1621 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag
     1681 gcgccgccgc accccctcta gcgggcgtgg ggcgaagcgg tgcggcgccg gcaggaagga
     1741 aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc
     1801 tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg
     1861 cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt
     1921 tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga
     1981 ccggtccacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     2041 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     2101 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     2161 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     2221 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccac gcatggccga
     2281 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     2341 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     2401 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     2461 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     2521 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     2581 cggtgcctga cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     2641 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     2701 catcgccttc tatcgccttc ttgacgagtt cttctgaggg gatcaattct ctagagctcg
     2761 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     2821 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     2881 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     2941 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     3001 ttctgaggcg gaaagaacca gctggggctc gactagagct tgcggaaccc ttaatataac
     3061 ttcgtataat gtatgctata cgaagttatg aattcgccac tgagaagtga cttcttgttc
     3121 agtagctctg gactcttgga gtccccagag accttgttca ggaaagggaa tgagaacatt
     3181 ccagcaattt tccccccacc tagccctccc aggttctatt tttagagtta tttctgatgg
     3241 agtccctgtg gagggaggag gctgggctga gggagggggt cctgcagggc ggggggctgg
     3301 gaaggtgggg agaggctgcc gagagccacc cgctatcccc agctctgggc agccccggga
     3361 cagtcacaca ccctggcctc gcggcccaag ctggcagccg tctgcagcca cagcttatgc
     3421 cagcccaggt ccagccagac acctgaggga cccactggtg ccttggagga agcaggagag
     3481 gtcagatggc accatgagct ggggcaggtg cagggaccgt ggcagcacct ggcagggcct
     3541 cagaacccat gccttgggca ccccggccat gaggccctga ggattgcagc ccaggagaag
     3601 cagggaaccg ccagggccac aggggcagag accagggcca gggtccccct gcagcccctt
     3661 agcccacccc ctcccagtaa gcagggctgc ttggctggct tcctttgcta cagacctgct
     3721 gctcacccag aagggcccac gggccctggt gacaaggtcg ttgtggctcc aggtccttgg
     3781 gggtcctgac acagagcctc ttctgcagca cccctgagga cagggtggct ccgctgggca
     3841 cccagcctag tgggcagacg agaacctagg ggctgcctgg gcctactgtg gcctgggagg
     3901 tcagcgggtg accctagcta ccctgtggct gggccagtct gcctgccacc caggccaaac
     3961 caatctgcac ctttcctgag agctccaccc agggctgggc tggggatggc tgggcctggg
     4021 gctggcatgg gctgtggctg cagaccactg ccagcttggg cctcgaggcc aggagctcac
     4081 cctccagctg gggacctggc cactggggca gccctgttcc tgaagctctg agctcacccc
     4141 ttccccatga ccacatcagc ccccctccac ccagagatgt cacagccccc agctagcccc
     4201 gcctccagag tgggggccaa ggctgggcag gcgggtggac ggccggacac tggccccgga
     4261 agaggaggga ggcggtggct gggatcggca gcagccgtcc atgggaacac ccagccggcc
     4321 ccactcgcac gggtagagac aggggcgccc tgctggagct gaggtatgtg agctcgcgcg
     4381 gggctgggcc aaagcggggc ccggtgggcc ggctgggagg ctgcccacca gtcagccatc
     4441 ggccaagctg ttgccctggc tgaccctgat ggccaacaag gccgtaggga gtgatgggca
     4501 gaggcccctt ctgggagggg agggtcagtg ctctgtgggg gaccgtgtgt tggagtggag
     4561 ggcagcagga ggagcccttt ggtgtccagg gactcctgga gctgccccag ccttccagga
     4621 cttgcagggc agctggcact ggctggtgct gggggctgag gagtgtcttt tgaggggcca
     4681 aattttctgt gacttctgtc ctgggggacc tctgacctga ggcctcagga gagggcaagg
     4741 ccgcccaccc aaaagagatg cagccatggt tcgcggtgcc ctcggctgcc ctgggccaga
     4801 gctggggcta gctttcacct tgttgagacc caggactctg tcccccaagc ctgtcttcgc
     4861 cagcgccttg accccacccc tcatatactg tgtcctggaa aacgtggaca cgggagaccg
     4921 cagccagggc gaggtatcgc ccctccatcc ccccaggccc aatgagaagc agttggccaa
     4981 ggtgatccag gtggcagagg cagcatcaga cccagtctcc tgtcaggcac caccttgggt
     5041 gccggtcccc agatgccctg gcggggagtg tgcatgctcc cggagccccc aggtcacccc
     5101 atgtgagcca ggcccacaga gcttggctct gcaatgcctg ctgggctgct gcccatgctc
     5161 caccccttct gggaagctaa aagacagccc ttcagtgtcc agagacctgc ctggccttgg
     5221 agcctcggtt tcacatgccc accgggctgg caggggcact cagctgcctc cagccccggc
     5281 ggtcaccctg gcattgggtc catctaactg ctccccagtc acaaggcagc tgctccccaa
     5341 gtctccccaa acctgctggc ccctctagaa gcctctgtcc attcctggag gaccgagggc
     5401 agcctgcatg ccatcccgca cacagccttc tgtctgggcg tcctgccttc acacatgctg
     5461 cacagggagg aaactcttat accacattcc ttaagcagag actgaagcct ggagccaggc
     5521 acatggcacg tgctcccacc cacccaggac acactgcggt gtggctgcct ccaggctggc
     5581 cccctagatt gcgtctgctc ctggcatgga taactggcgc ctttgcctgg ccgttggggc
     5641 agtgtttgcc ttcccctgtc ggcagcaaat atttactgtc ctccgtctcc aggactctcc
     5701 aggcctgagc agaccccggg gggatgagtg tggactcagc ggtgctgagg gtagccccct
     5761 gcccttcggg tcctggtgcc cagcaggggt ccagcccagg gaagagactg aggccaggac
     5821 aggcagtgtt taagcctgag tttctgggaa aggtagccct gggcagaact tgggccgaac
     5881 gttggccagt gtctctctcc agccaggctg tgaggtagct gtttccagga tgggcacctt
     5941 tccacaccca gcaatgtggc caggagccgc cattcacggg tgcgaccagc agatggcatc
     6001 agagcctcac ttttgatgca ctccggccac cagccacggg tccaggttct ggccaccacc
     6061 cagggtctga gcagctgcat cctgcccctg ccgggcactc ccgggggctg tggggcctgt
     6121 gggggccctg ccagacactc ttgggggctg tggggggccc tgccaggcac tcccagggac
     6181 tatgggggct gtggggggcc ctgctgggca ctctgaaggg catggggctt aggaatgaga
     6241 ggagctgtct gatgatgatg gtgggggcat tgcagaggcc cccggcctgc tcaggtccag
     6301 tctcggcccc taagtcaagc ctcaggccag cctctcacca gcctgggttt ctcagagggc
     6361 cgggacaaat gttctgggtc tctaatattc caagaaagcc tctggctgga ctctgagccc
     6421 cacctgcgag cccctagaat cacagagagc tagggtgaga agaccagggg gactccgtcc
     6481 caccctcgtc gtggctgagc ccactgtggc cggtggtgga ccaggctgtg gcctttgctg
     6541 agggtcccca gggcccctgg gggctactga ggctggaggc cagcggtggc caggagggtc
     6601 cctccctcag ccactcaagc cagaaggtcg agtcctggtt tctatgtgag gagggggctt
     6661 caggggctgg gacctggggg caccgaaggc ctggagctgg ggtccaggcg gctgagggtt
     6721 agtgcgttcc cacgctcccc tccgccagcg ccgtgaggag agggaggtcc actctggaaa
     6781 gaatgtttga gggcaggggt agacagggtc tgggaacgcg gagatgacac actggggtgc
     6841 cgcctccgca ccctccgccc ttcctcccac tattgtttct ggcctcggcc gcaggaacag
     6901 caaggcagac tagtggatcc tctagagtcg agcagtgtgg ttttcaagag gaagcaaaaa
     6961 gcctctccac ccaggcctgg aatgtttcca cccaatgtcg agcagtgtgg ttttgcaaga
     7021 ggaagcaaaa agcctctcca cccaggcctg gaatgtttcc acccaatgtc gagcaaaccc
     7081 cgcccagcgt cttgtcattg gcgaattcga acacgcagat gcagtcgggg cggcgcggtc
     7141 ccaggtccac ttcgcatatt aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca
     7201 gcgacccgct taacagcgtc aacagcgtgc cgcagatctt ggtggcgtga aactcccgca
     7261 cctcttcggc cagcgccttg tagaagcgcg tatggcttcg taccccggcc atcagcacgc
     7321 gtctgcgttc gaccaggctg cgcgttctcg cggccatagc aaccgacgta cggcgttgcg
     7381 ccctcgccgg cagcaagaag ccacggaagt ccgcccggag cagaaaatgc ccacgctact
     7441 gcgggtttat atagacggtc cccacgggat ggggaaaacc accaccacgc aactgctggt
     7501 ggccctgggt tcgcgcgacg atatcgtcta cgtacccgag ccgatgactt actggcgggt
     7561 gctgggggct tccgagacaa tcgcgaacat ctacaccaca caacaccgcc ttgaccaggg
     7621 tgagatatcg gccggggacg cggcggtggt aatgacaagc gcccagataa caatgggcat
     7681 gccttatgcc gtgaccgacg ccgttctggc tcctcatatc gggggggagg ctgggagctc
     7741 acatgccccg cccccggccc tcaccctcat cttcgaccgc catcccatcg ccgccctcct
     7801 gtgctacccg gccgcgcgat accttatggg cagcatgacc ccccaggccg tgctggcgtt
     7861 cgtggccctc atcccgccga ccttgcccgg cacaaacatc gtgttggggg cccttccgga
     7921 ggacagacac atcgaccgcc tggccaaacg ccagcgcccc ggcgagcggc ttgacctggc
     7981 tatgctggcc gcgattcgcc gcgtttacgg gctgcttgcc aatacggtgc ggtatctgca
     8041 gggcggcggg tcgtggcggg aggattgggg acagctttcg gggacggccg tgccgcccca
     8101 gggtgccgag ccccagagca acgcgggccc acgaccccat atcggggaca cgttatttac
     8161 cctgtttcgg gcccccgagt tgctggcccc caacggcgac ctgtacaacg tgtttgcctg
     8221 ggccttggac gtcttggcca aacgcctccg tcccatgcac gtctttatcc tggattacga
     8281 ccaatcgccc gccggctgcc gggacgccct gctgcaactt acctccggga tgatccagac
     8341 ccacgtcacc accccaggct ccataccgac gatctgcgac ctggcgcgca cgtttgcccg
     8401 ggagatgggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     8461 tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc
     8521 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     8581 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     8641 ctcgcagcca acgtcggggc ggcaggccct gccatagcca cgggccccgt gggttaggga
     8701 cggggtcccc catggggaat ggtttatggt tcgtgggggt tattattttg ggcgttgcgt
     8761 ggggtcagtc cacgactgga ctgagcagac agacccatgg tttttggatg gcctgggcat
     8821 ggaccgcatg tactggcgcg acacgaacac cgggcgtctg tggctgccaa acacccccga
     8881 cccccaaaaa ccaccgcgcg gatttctggc gccgccggac gaactaaacc tgactacggc
     8941 atctctgccc cttcttcgct ggtacgagga gcgcttttgt tttgtattgg tcaccacggc
     9001 cgagtttccg cgggaccccg gccagatcaa gctagcttat cgataccgtc gatcgacctc
     9061 gagggggggc ccgcagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat
     9121 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     9181 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     9241 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     9301 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     9361 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9421 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9481 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     9541 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9601 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9661 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9721 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9781 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9841 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9901 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9961 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    10021 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    10081 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    10141 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    10201 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    10261 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    10321 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    10381 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    10441 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    10501 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    10561 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    10621 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10681 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10741 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10801 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10861 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10921 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10981 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    11041 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    11101 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    11161 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    11221 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctstgk
    11281 aatgactgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    11341 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    11401 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    11461 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    11521 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta
    11581 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
    11641 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
    11701 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
    11761 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
    11821 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
    11881 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
    11941 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
    12001 acaaaatatt aacgcttaca atttccattc gccattcagg ctgcgcaact gttgggaagg
    12061 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    12121 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    12181 tgaattgtaa tacgactcac tatagggcga attggagctc caccgcggtg gatgcatgtc
    12241 gacaaggaaa aaagcggccg cccctctagc ctcatcctca taaaaggtct catcattttc
    12301 cctccagcct cttatgcact ggggaaactg aggccagggg ctatgtgtcc agcggacagg
    12361 ggtgctgaat tccacccaca ggcttaggga tatggtcaag gaaagcttcc tggaggaggc
    12421 ccagtggagg ttcagggagg gatggggtgc ccggcagtct ctagtggaaa aggcgcctag
    12481 cctatctccc ccatgaaccc cctcacccag ccctggaaga ggcctcagtg tcccgcctgt
    12541 gaccagttgg ctcagaaaag ccctgggagc tctgagccac tgtgaaggtg gaaacgcggc
    12601 ccctggcctc ccctctcctg gaggctgcag actctgcccg ccagttgacg agggctctgc
    12661 cgctctcctc cccaggagct atgcctcacg catccagcgc cccttctccg tgaagttcga
    12721 cccgtacacg ctggccatcg acgtgctgga cagcccccag gccgtgcggc gctccctgga
    12781 gggtgtccag gatgagctgg acacccttgc ccatgcgctg agtgccattg gctaggtgca
    12841 cggcgtccct gagggccctt cccaacctcc cctggtcctg cactgtcccg gagctcaggc
    12901 cctggtgagg ggctgggtcc cgggtgcccc ccatgccctc cctgctgcca ggctcccact
    12961 gcccctgcac ctgcttctca gcgcaacagc tgtgtgtgcc cgtggtgagg ttgtgctgcc
    13021 tgtggtgagg tcctgtcctg gctcccaggg tcctgggggc tgctgcactg ccctccgccc
    13081 ttccctgaca ctgtctgctg ccccaatcac cgtcacaata aaagaaactg tggtctctac
    13141 acctgcctgg ccccacatct gtgccacaga gacagaccct gggatcctca gactcccaca
    13201 cccccacccc agcctcactc agaggtttcg ccctggcctc cttcctcctc tgggagatgg
    13261 ctggccgccc tggccaggca gctggcccct ccgggcctgg tttccccgct caccctgagg
    13321 ccccgcccag ctctgagccc caagcagctc cagaggctcg ggcaccctgg ccgagctgcc
    13381 ccatctccgt ggggtgccct cccaaggtgg ggagccacgt gacagtggga gggcctctct
    13441 caggcctggc agggagcagg ggtcacaaac tgtgctggct gggggtggtc tcagaggtgg
    13501 gcctgcaggc ctaaccctcc ctgctgacag ggctcccagc ccttgagaga aacagggatg
    13561 gaggaacagc tgccctgatg ccctcaccca cccggagcag gccctgcgaa ccaaggggaa
    13621 cctcagtgtg gcccccagca tgtgtgctga tggggagggt ctggctgagc tggtgcccag
    13681 gcagatggtc tgggcctgtc tccccagcga ggcaggatgg gggctggatt tcagactctg
    13741 taagatgccc ctggcttact cgaggggcct ggacattgcc ctccagagag agcacccaac
    13801 accctccagg cttgaccggc cagggtgtcc ccttcctacc ttggagagag cagccccagg
    13861 gcatcctgca gggggtgctg ggacaccagc tggccttcaa ggtctctgcc tccctccagc
    13921 caccccacta cacgctgctg ggatcctgga tctcagctcc ccggccgaca acactggcaa
    13981 actcctactc atccacgaag gccctcctgg gcatggtggt ccttcccagc ctggcagtct
    14041 gttcctcaca caccttgtta gtgcccagcc cctgaggttg cagctggggg tgtctctgaa
    14101 gggctgtgag cccccaggaa gccctgggga agtgcctgcc ttgcctcccc ccggccctgc
    14161 cagcgcctgg ctctgccctc ctacctgggc tccccccatc cagcctccct ccctacacac
    14221 tcctctcaag gaggcaccca tgtcctctcc agctgccggg cctcagagca ctgtggcgtc
    14281 ctggggcagc caccgcatgt cctgctgtgg catggctcag ggtggaaagg gcggaaggga
    14341 ggggtcctgc agatagctgg tgcccactac caaacccgct cggggcagga gagccaaagg
    14401 ctgggtgtgt gcagagcggc cccgagaggt tccgaggctg aggccagggt gggacatagg
    14461 gatgcgaggg gccggggcac aggatactcc aacctgcctg cccccatggt ctcatcctcc
    14521 tgcttctggg acctcctgat cctgcccctg gtgctaagag gcaggtaggg gctgcaggca
    14581 gcagggctcg gagcccatgc cccctcacca tgggtcaggc tggacctcca ggtgcctgtt
    14641 ctggggagct gggagggccg gaggggtgta ccccaggggc tcagcccaga tgacactatg
    14701 ggggtgatgg tgtcatggga cctggccagg agaggggaga tgggctccca gaagaggagt
    14761 gggggctgag agggtgcctg gggggccagg acggagctgg gccagtgcac agcttcccac
    14821 acctgcccac ccccagagtc ctgccgccac ccccagatca cacggaagat gaggtccgag
    14881 tggcctgctg aggacttgct gcttgtcccc aggtccccag gtcatgccct ccttctgcca
    14941 ccctggggag ctgagggcct cagctggggc tgctgtccta aggcagggtg ggaactaggc
    15001 agccagcagg gaggggaccc ctccctcact cccactctcc cacccccacc accttggccc
    15061 atccatggcg gcatcttggg ccatccggga ctggggacag gggtcctggg gacaggggtg
    15121 tggggacagg ggtcctgggg acaggggtct ggggacaggg gtcctgggga caggggtgtg
    15181 gggacagggg tgtggggaca ggggtgtggg gacaggggtc ctggggacag gggtctgggg
    15241 acaggggtct gaggacaggg gtgtggggac aggggtntgg ggacaggggt gtggggacag
    15301 gggtstgggg acaggggtct ggggacaggg gtccggggga caggggtgtg gggacagggg
    15361 tgtggggaca ggggtgtggg gacaggggtc tggggacagg ggtgtgggga caggggtcct
    15421 ggggacaggg gtgtggggat aggggtgtgg ggacaggggt gtggggacag gggtgtgggg
    15481 acaggggtct ggggacagca gcgcaaagag ccccgccctg cagcctccag ctctcctggt
    15541 ctaatgtgga aagtggccca ggtgagggct ttgctctcct ggagacattt gcccccagct
    15601 gtgagcaggg acaggtctgg ccaccgggcc cctggttaag actctaatga cccgctggtc
    15661 ctgaggaaga ggtgctgacg accaaggaga tcttcccaca gacccagcac cagggaaatg
    15721 gtccggaaat tgcagcctca gcccccagcc atctgccgac ccccccaccc caggccctaa
    15781 tgggccaggc ggcaggggtt gagaggtagg ggagatgggc tctgagacta taaagccagc
    15841 gggggcccag cagccctcag ccctccagga caggctgcat cagaagaggc catcaagcag
    15901 gtctgttcca agggcctttg cgtcaggtgg gctcaggatt ccagggtggc tggaccccag
    15961 gccccagctc tgcagcaggg aggacgtggc tgggctcgtg aagcatgtgg gggtgagccc
    16021 aggggcccca aggcagggca cctggccttc agcctgcctc agccctgcct gtctcccaga
    16081 tcactgtcct tctgccatgg ccctgtggat gcgcctcctg cccctgctgg cgctgctggc
    16141 cctctgggga cctgacccag ccgcagcctt tgtgaaccaa cacctgtgcg gctcacacct
    16201 ggtggaagct ctctacctag tgtgcgggga acgaggcttc ttctacacac ccaagacccg
    16261 ccgggaggca gaggacctgc agggtgagcc aactgcccat tgctgcccct ggccgccccc
    16321 agccaccccc tgctcctggc gctcccaccc agcatgggca gaagggggca ggaggctgcc
    16381 acccagcagg gggtcaggtg cactttttta aaaagaagtt ctcttggtca cgtcctaaaa
    16441 gtgaccagct ccctgtggcc cagtcagaat ctcagcctga ggacggtgtt ggcttcggca
    16501 gccccgagat acatcagagg gtgggcacgc tcctccctcc actcgcccct caaacaaatg
    16561 ccccgcagcc catttctcca ccctcatttg atgaccgcag attcaagtgt tttgttaagt
    16621 aaagtcctgg gtgacctggg gtcacagggt gccccacgct gcctgcctct gggcgaacac
    16681 cccatcacgc ccggaggagg gcgtggctgc ctgcctgagt gggccagacc cctgtcgcca
    16741 ggcctcacgg cagctccata gtcaggagat ggggaagatg ctggggacag gccctgggga
    16801 gaagtactgg gatcacctgt tcaggctccc actgtgacgc tgccccgggg cgggggaagg
    16861 aggtgggaca tgtgggcgtt ggggcctgta ggtccacacc cagtgtgggt gaccctccct
    16921 ctaacctggg tccagcccgg ctggagatgg gtgggagtgc gacctagggc tggcgggcag
    16981 gcgggcactg tgtctccctg actgtgtcct cctgtgtccc tctgcctcgc cgctgttccg
    17041 gaacctgctc tgcgcggcac gtcctggcag tggggcaggt ggagctgggc gggggccctg
    17101 gtgcaggcag cctgcagccc ttggccctgg aggggtccct gcagaagcgt ggcattgtgg
    17161 aacaatgctg taccagcatc tgctccctct accagctgga gaactactgc aacggtacct
    17221 acccatacga tgttccagat tacgctggca gtggagaggg cagaggaagt ctgctaacat
    17281 gcggtgacgt cgaggagaat cctggcccaa tggaagacgc caaaaacata aagaaaggcc
    17341 cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga
    17401 agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca
    17461 tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg
    17521 ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc
    17581 cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg
    17641 aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa
    17701 aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta
    17761 tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgctc gtcacatctc
    17821 atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga
    17881 caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc
    17941 ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa
    18001 tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta
    18061 ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag
    18121 agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc
    18181 tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg
    18241 aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt
    18301 tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca gctattctga
    18361 ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag
    18421 cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt
    18481 gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct
    18541 tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac
    18601 acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc
    18661 ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag
    18721 gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa
    18781 agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa
    18841 agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg
    18901 acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgg
    18961 tcgacgaggg cagaggcagc ctgctgacat gtggcgacgt ggaagagaac cctggcccca
    19021 tgcatatgcc acctcctcgc ctcctcttct tcctcctctt cctcaccccc atggaagtca
    19081 ggcccgagga acctctagtg gtgaaggtgg aagagggaga taacgctgtg ctgcagtgcc
    19141 tcaaggggac ctcagatggc cccactcagc agctgacctg gtctcgggag tccccgctta
    19201 aacccttctt aaaactcagc ctggggctgc caggcctggg aatccacatg aggcccctgg
    19261 ccatctggct tttcatcttc aacgtctctc aacagatggg gggcttctac ctgtgccagc
    19321 cggggccccc ctctgagaag gcctggcagc ctggctggac agtcaatgtg gagggcagcg
    19381 gggagctgtt ccggtggaat gtttcggacc taggtggcct gggctgtggc ctgaagaaca
    19441 ggtcctcaga gggccccagc tccccttccg ggaagctcat gagccccaag ctgtatgtgt
    19501 gggccaaaga ccgccctgag atctgggagg gagagcctcc gtgtgtccca ccgagggaca
    19561 gcctgaacca gagcctcagc caggacctca ccatggcccc tggctccaca ctctggctgt
    19621 cctgtggggt accccctgac tctgtgtcca ggggccccct ctcctggacc catgtgcacc
    19681 ccaaggggcc taagtcattg ctgagcctag agctgaagga cgatcgcccg gccagagata
    19741 tgtgggtaat ggagacgggt ctgttgttgc cccgggccac agctcaagac gctggaaagt
    19801 attattgtca ccgtggcaac ctgaccatgt cattccacct ggagatcact gctcggccag
    19861 tactatggca ctggctgctg aggactggtg gctggaaggt ctcagctgtg actttggctt
    19921 atctgatctt ctgcctgtgt tcccttgtgg gcattcttca tcttcaaaga gccctggtcc
    19981 tgaggaggaa aagaaagcga atgactgacc ccaccaggag attcttcaaa gtgacgcctc
    20041 ccccaggaag cgggccccag aaccagtacg ggaacgtgct gtctctcccc acacccacct
    20101 caggcctcgg acgcgcccag cgttgggccg caggcctggg gggcactgcc ccgtcttatg
    20161 gaaacccgag cagcgacgtc caggcggatg gagccttggg gtcccggagc ccgccgggag
    20221 tgggcccaga agaagaggaa ggggagggct atgaggaacc tgacagtgag gaggactccg
    20281 agttctatga gaacgactcc aaccttgggc aggaccagct ctcccaggat ggcagcggct
    20341 acgagaaccc tgaggatgag cccctgggtc ctgaggatga agactccttc tccaacgctg
    20401 agtcttatga gaacgaggat gaagagctga cccagccggt cgccaggaca atggacttcc
    20461 tgagccctca tgggtcagcc tgggacccca gccgggaagc aacctccctg gggtcccagt
    20521 cctatgagga tatgagagga atcctgtatg cagcccccca gctccactcc attcggggcc
    20581 agcctggacc caatcatgag gaagatgcag actcttatga gaacatggat aatcccgatg
    20641 ggccagaccc agcctgggga ggagggggcc gcatgggcac ctggagcacc aggggcagtg
    20701 gagagggcag aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct ggcccagtga
    20761 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
    20821 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
    20881 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
    20941 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
    21001 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
    21061 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
    21121 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
    21181 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
    21241 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
    21301 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
    21361 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
    21421 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taattaatta
    21481 a
//
