LOCUS       sequence-31306-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EPHB4
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             372..389
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(442..460)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      456..503
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      540..898
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(627..646)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     837..858
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attcctctga atattttgtt tactttaaga aggagatata ccatgcatca tcatcatcat
       61 cacagcagcg gcagagaaaa cttgtatttc cagggccacc ctctcctgga ccagcggcag
      121 cctcactact cagcttttgg ctctgtgggc gagtggcttc gggccatcaa aatgggaaga
      181 tacgaagaaa gtttcgcagc cgctggcttt ggctccttcg agctggtcag ccagatctct
      241 gctgaggacc tgctccgaat cggagtcact ctggcgggac accagaagaa aatcttggcc
      301 agtgtccagc acatgaagtc ccaggccaag ccgggaaccc cgtgatgacg aagcttgcgg
      361 ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg
      421 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
      481 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga
      541 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
      601 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
      661 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
      721 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
      781 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
      841 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
      901 agggattttg cg
//
